Leighton Pritchard

Publications (3)5.79 Total impact

• Source

  Available from: psu.edu

  Article: A general model of error-prone PCR.

  Leighton Pritchard, Dave Corne, Douglas Kell, Jem Rowland, Mike Winson
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  ABSTRACT: In this paper, we generalize a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.
  Journal of Theoretical Biology 07/2005; 234(4):497-509. · 2.21 Impact Factor
• Source

  Available from: scri.ac.uk

  Article: Schemes of flux control in a model of Saccharomyces cerevisiae glycolysis.

  Leighton Pritchard, Douglas B Kell
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  ABSTRACT: We used parameter scanning to emulate changes to the limiting rate for steps in a fitted model of glucose-derepressed yeast glycolysis. Three flux-control regimes were observed, two of which were under the dominant control of hexose transport, in accordance with various experimental studies and other model predictions. A third control regime in which phosphofructokinase exerted dominant glycolytic flux control was also found, but it appeared to be physiologically unreachable by this model, and all realistically obtainable flux control regimes featured hexose transport as a step involving high flux control.
  European Journal of Biochemistry 09/2002; 269(16):3894-904. · 3.58 Impact Factor
• Article: A general model of error-prone PCR

  Leighton Pritchard, D. Corne, Douglas B Kell, Jem Rowland, Mike. K. Winson
  [show abstract] [hide abstract]
  ABSTRACT: In this paper, we generalise a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.