Publications (4)13.7 Total impact
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Article: C/EBP and C-Myb sites are important for the functional activity of the human myeloperoxidase upstream enhancer.
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ABSTRACT: Myeloperoxidase (MPO), an enzyme active against bacterial and fungal infections, is expressed specifically in myeloblasts and promyelocytes and minimal in other cell types. We recently identified and partially characterized an upstream enhancer located between -4100 and -3844 bp of the MPO gene. We showed that an AML1 site contributes to enhancer activity and specificity. We now demonstrate three additional footprints within the MPO enhancer and provide evidence that C/EBP and c-Myb sites contribute to its functional, tissue-specific activity. This distal enhancer appears to play an important role in the control of MPO transcription during differentiation of myeloid cells.Biochemical and Biophysical Research Communications 07/2008; 371(2):309-14. · 2.48 Impact Factor -
Article: Free oxygen radicals in whole blood correlate strongly with high-sensitivity C-reactive protein.
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ABSTRACT: Increased concentrations of reactive oxygen molecules are believed to be a driving force in inflammation. Although evident in tissue culture and animal models, it has been difficult to link reactive oxygen species (ROS) and inflammatory markers in humans. In patients recruited to represent a broad spectrum of risk factors, we investigated the relationship between the plasma concentration of oxygen radicals and high-sensitivity C-reactive protein (hs-CRP), utilizing a new chemistry with an easily oxidized chromophore. ROS and hs-CRP were measured in blood from 59 fasting subjects selected to have variable risk predicted by classical risk factors. ROS were determined using the free oxygen radical monitor, which is an indirect colorimetric assay for the concentration of hydroperoxides in whole blood. Using log transformation, the correlation between ROS and hs-CRP was r = 0.505 (P < 0.0001). This relationship between ROS and hs-CRP was comparable (r = 0.527, P = 0.001) in the subgroup not currently on statin therapy (n = 39). ROS were not correlated with Framingham risk, r = -0.027 (P = 0.84). ROS directly measured in human blood correlates strongly with hs-CRP.Journal of Clinical Lipidology 12/2007; 1(6):593-8. · 1.58 Impact Factor -
Article: Paraprotein interference in automated chemistry analyzers.
Clinical Chemistry 07/2005; 51(6):1077-8. · 7.91 Impact Factor -
Article: Immunohistochemical detection of carcinoembryonic antigen in esophageal carcinomas: a comparison with other gastrointestinal neoplasms.
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ABSTRACT: Although the prognostic value of Carcinoembryonic antigen (CEA) in colorectal cancer follow-up is well known, CEA expression in esophageal cancer is not widely recognized and studies correlating tissue CEA expression in stomach cancers with tumor differentiation have yielded contradictory results. We compared the CEA expression in esophageal, gastric and colorectal carcinomas in order to elucidate its diagnostic and prognostic significance and to evaluate the potential role of tissue CEA localization for the clinical evaluation of esophageal carcinomas. CEA expression in 84 biopsies of carcinomas of the gastrointestinal tract (38 colorectal, 22 gastric, 24 esophageal) was evaluated by immunohistochemistry. Sixty-two percent of the squamous carcinomas of esophagus, all five esophageal adenocarcinomas arising in Barrett esophagus, 86 percent of gastric adenocarcinomas and 89 percent of colorectal adenocarcinomas were CEA-positive. In colorectal and gastric adenocarcinomas, CEA staining was present, usually at the luminal membrane of neoplastic glands and in intraluminal material. Signet ring cells were strongly positive for CEA. In esophageal carcinomas staining was mostly cytoplasmic and in the better differentiated tumors it was particularly prominent at the center of epithelial pearls. Intensity of staining was highest in well-differentiated carcinomas and lowest in poorly-differentiated carcinomas. A clear correlation was seen between the degree of tumor differentiation and CEA expression for carcinomas of the esophagus, stomach and colon. Our results support the potential usefulness of CEA for monitoring the recurrence of gastric or esophageal tumors. Immunohistochemical determination of tumor CEA content could be a useful adjunct for the management of gastrointestinal carcinomas, by improving interpretation of serum CEA levels.Anticancer research 22(3):1849-57. · 1.73 Impact Factor
