Danielle Palma de Oliveira

Publications (3)6.02 Total impact

• Article: Evaluation of dicloran's contribution to the mutagenic activity of Cristais River, Brazil, water samples.

  Danielle Palma de Oliveira, Maureen Sakagami, Sarah Warren, Fábio Kummrow, Gisela de Aragão Umbuzeiro
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  ABSTRACT: 2,6-Dichloro-4-nitroaniline (dicloran) is a mutagenic aromatic amine used as an agricultural fungicide and in the synthesis of disperse dyes. It is a known mutagen (Salmonella/microsome assay) in strains TA98 and TA100. Dicloran was initially detected, but not quantified, in the Cristais River, Brazil. The objective of the present study was to estimate the contribution of dicloran to mutagenic activity in samples from this river. Dicloran was found in the raw water at 0.14 microg/L but not in the treated water. Comparison of mutagenic potencies in Salmonella strain YG1041 for dicloran and the river water sample indicated that dicloran contributed less than 0.1% of total mutagenic activity.
  Environmental Toxicology and Chemistry 06/2009; 28(9):1881-4. · 2.81 Impact Factor
• Article: Evaluation of the Presence of Mutagenic Dyes in Sediments from Cristais River

  Danielle Palma De Oliveira, Mônica Luisa Kuhlmann, Gisela De Aragão Umbuzeiro
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  ABSTRACT: Azo dyes are largely used by coloring textiles and can contaminate the aquatic environment, including the sediment, through their release through effluent discharges. In this work the presence of mutagenic azo dyes was evaluated using Thin Layer Chromatography in sediment samples of the Cristais River upstream and downstream of an azo dye processing plant discharge area. Mutagenicity of the sediment samples was also analyzed using the Salmonella/microsome assay with the strain YG1041 in the presence and absence of S9. Extracts of benthic organisms collected in the same area were analyzed for the presence of dyes. The dyes CI Disperse Blue 373 and CI Disperse Orange 37 as well as three unknown fluorescent compounds were detected only in the sediment samples collected downstream of the industrial discharge. Activity was detected with the Salmonella assay in the three samples analyzed but higher values were obtained after the azo dye processing plant when compared to the reference site. This effect could be partially explained by the presence of the mutagenic dyes detected, considering their mutagenic potencies. No dyes were found in the extracts of the organisms. Further studies should be performed to evaluate the fate and effects of these dyes in the sediment and in the aquatic community and their potential to be transferred to the water column.
  Soil & Sediment Contamination. 09/2006; 15(5):455-462.
• Article: The contribution of azo dyes to the mutagenic activity of the Cristais River.

  Gisela de Aragão Umbuzeiro, Harold S Freeman, Sarah H Warren, Danielle Palma de Oliveira, Yoshiyasu Terao, Tetsushi Watanabe, Larry D Claxton
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  ABSTRACT: To verify whether dyes emitted within the discharge of a dye processing plant were contributing to the mutagenicity repeatedly found in the Cristais River, Sao Paulo, Brazil, we chemically characterized the following mutagenic samples: the treated industrial effluent, raw and treated water, and the sludge produced by a Drinking Water Treatment Plant (DWTP) located approximately 6 km from the industrial discharge. Considering that 20% of the dyes used for coloring activities might be lost to wastewaters and knowing that several dyes have mutagenic activity, we decided to analyze the samples for the presence of dyes. Thin layer chromatographic analysis indicated the presence of three prevalent dyes in all samples, except for the drinking water. This combination of dyes corresponded to a commercial product used by the industry, and it tested positive in the Salmonella assay. The structures of the dye components were determined using proton magnetic resonance and mass spectrometric (MS) methods, and the dyes were tested for mutagenicity. The blue component was identified as the C.I. Disperse Blue 373, the violet as C.I. Disperse Violet 93, and the orange as C.I. Disperse Orange 37. The dyes showed mutagenic responses of 6300, 4600, and 280 revertants/microg for YG1041 with S9 respectively. A bioassay-directed fractionation/chemical analysis showed that the C.I. Disperse Blue 373 contributed 55% of the mutagenic activity of the DWTP sludge. We showed that these dyes contributed to the mutagenic activity found in the Cristais River environmental samples analyzed and are indirectly affecting the quality of the related drinking water. Therefore, we believe that this type of discharge should be more thoroughly characterized chemically and toxicologically. Additionally, human and ecological risks associated with the release of dye processing plant effluents should be more fully investigated, especially where the resultant water is taken for human consumption.
  Chemosphere 07/2005; 60(1):55-64. · 3.21 Impact Factor