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ABSTRACT: It is suggested that dental pulp stem cells are involved in tooth regeneration and play an important role in maintaining pulp homeostasis. Previously, normal dental pulps were more widely used for experimental models than carious dental pulps. The aim of this study was to isolate and culture the dental pulp stem cells from carious and normal teeth and to evaluate stem cell parameters.
Pulp tissues were obtained and dissociated from normal and carious teeth. Single-cell suspensions were seeded into 6-well plates and purified by collecting multiple colonies. Normal dental pulp stem cells (DPSCs) and carious dental pulp stem cells (CDPSCs) were compared for morphologic appearance and for their capacity to differentiate into 3 lineages. Colony-forming and MTT assays, cell cycle analysis, gene expression, and alkaline phosphatase activity were also evaluated.
Stem cells were cultured successfully from normal and carious dental pulps. CDPSCs displayed increased proliferation ability compared with DPSCs. CDPSCs also showed enhanced ALP activity, mineralization ability, and expression of osteogenesis/dentinogenesis-related genes. All cultures differentiated into 3 cell types.
Our data suggest that caries as a local microenvironment should be taken into account when DPSCs are intended to be used for investigations and application. Furthermore, the mechanism of the underlying changes in cell properties requires further study.
Journal of endodontics 06/2012; 38(6):796-802. · 2.95 Impact Factor
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Wei Li,
He Zhao,
Weizhu Qian,
Huafei Li,
Li Zhang,
Zengwei Ye,
Ge Zhang,
Mao Xia,
Jinfeng Li, Jie Gao,
Bohua Li,
Geng Kou,
Jianxin Dai,
Hao Wang,
Yajun Guo
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ABSTRACT: Micelles with high in vivo serum stability and intratumor accumulation post intravenous (i.v.) injection are highly desired for promoting chemotherapy. Herein, we finely synthesized and tailored well-defined anti-Her2 antibody Fab fragment conjugated immunomicelles (FCIMs), which showed interesting dual targeting function. The thermosensitive poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide)(118) (PID(118)) shell with volume phase transition temperature (VPTT: 39 °C) and the anchored anti-Her2 Fab moiety contributed to the passive and active targeting, respectively. The doxorubicin (DOX) loading capacity of such FCIMs was successfully increased about 2 times by physically enhanced hydrophobicity of inner reservoir without structural deformation. The cellular uptake and intracellular accumulation of DOX by temperature regulated passive and antibody navigated active targeting was 4 times of Doxil. The cytotoxicity assay against Her2 overexpression gastric cancer cells (N87s) showed that the IC50 of the FCIMs was ≈ 9 times lower than that of Doxil under cooperatively targeting by Fab at T > VPTT. FCIMs showed high serum stability by increasing the corona PID(118) chain density (S(corona)/N(agg)). In vivo tissue distribution was evaluated in Balb/c nude mice bearing gastric cancer. As observed by the IVIS(®) imaging system, the intratumor accumulation of such finely tailored FCIMs system was obviously promoted 24 h post i.v. administration. Due to the high stability and super-targeting, the in vivo xenografted gastric tumor growth was significantly inhibited with relative tumor volume <2 which was much smaller than ≈ 5 of the control. Consequently, such finely tailored FCIMs with anti-Her2 active and temperature regulated passive dual tumor-targeting function show high potent in chemotherapy.
Biomaterials 04/2012; 33(21):5349-62. · 7.40 Impact Factor
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ABSTRACT: Members of the let-7 family have been shown to play a critical role in cell differentiation and tumorigenesis. However, potential targets of let-7 are still unclear. In the current study, we used bioinformatic analysis combined with DNA sequence analysis to identify potential let-7 targets. We discovered that dentin matrix protein 1 (DMP1), which is a non-collagenous protein essential in the mineralization of dentin and bone, has a let-7 binding site in its 3'-untranslated region. Furthermore, reporter assays demonstrated that the DMP1 3'-untranslated region can be regulated directly by the members of let-7. Gene expression levels of let-7 and DMP1 were validated by qRT-PCR of dental pulp cells cultured in a mineralizing medium. Our results suggest that DMP1 is regulated post-transcriptionally by let-7 during odontoblast differentiation.
International Journal of Molecular Medicine 04/2012; 30(2):295-301. · 1.98 Impact Factor
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ABSTRACT: An efficient methodology for the oxidative addition reaction of various aldehydes with 5,5-dimethylcyclohexane-1,3-dione and 1,3-indandione to selectively afford spiro dihydrofuran and cyclopropane derivatives, promoted by molecular iodine and dimethylaminopyridine under mechanical milling conditions, has been demonstrated. The products were obtained in good to excellent yields. A possible mechanism of this unusual reaction process is proposed.
Organic Letters 05/2009; 11(11):2385-8. · 5.86 Impact Factor
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ABSTRACT: Oxone is found to be an effective oxidant for the oxidative amidation of aldehydes with anilines to furnish amides in a one-pot process under mechanical milling conditions.
The Journal of Organic Chemistry 05/2008; 73(7):2955-8. · 4.45 Impact Factor
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ABSTRACT: Under mechanical milling conditions, direct reductive benzylizations of malononitrile and 4-methylaniline by aromatic aldehydes were achieved using a Hantzsch 1,4-dihydropyridine as the reductant.
Organic & Biomolecular Chemistry 06/2005; 3(9):1617-9. · 3.70 Impact Factor
