[Show abstract][Hide abstract] ABSTRACT: The electrophysiological activity taking place through the membrane of neuronlike NG108-15 cells upon stimulation through the single walled carbon nanotube (SWNT) coatings was investigated. A special substrate was designed in order to obtain electrophysiological measurements on the communication between neuron cells and SWNT LBL films. The successful electrical stimulation of NG108-15 cells depends on the ability of the cells to attach to the surface of nanotube films. Electrophysiological measurements were conducted using a voltage/patch clamp technique. Cells were voltage clamped using single patch electrodes in the whole-cell mode. The potential between the pipette electrode and the reference electrode in the bath was held at -60 mV. It was observed that SWNt can be incorporated into LBL multilayers with sufficiently high electrical conductivity to electrically stimulate excitable neuron cells.
[Show abstract][Hide abstract] ABSTRACT: A freestanding single-walled carbon nanotubes(SWNT)-polymer thin film membranes that are mechanically compatible with tissues under constant flexural and shear stress were analyzed. The freestanding structures of SWNTs have been found to guide and direct the outgrowth of neurites from NG108-15 neuroblastoma x glioma hybrid culture cells, and can potentially support cell-to-cell communications. It was observed that the layer-by-layer(LBL) SWNT composites can be used as favorable substrates for neuronal differentiation and attachment. The LBL films are also capable of incorporating proteins or various drugs, stimulating neural growth of establishing synaptic contacts while retaining the unique properties of SWNTs.