Publications (8)9.88 Total impact
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Article: Urethral distension as a novel method to simulate sphincter insufficiency in the porcine animal model.
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ABSTRACT: To describe a novel animal model of intrinsic sphincter deficiency. The study was carried out on 10 female pigs. Injury to the urethral sphincter was induced by distension of the urethra. This was obtained by using the balloon of an 18-F Dufour catheter for 5 min followed by its retraction through the urethra without draining the balloon. The urethral pressure profile was evaluated before injury, immediately postinjury and at day 28 postinjury in the experimental group (n = 5), and on day 1 and day 28 in the control uninjured group (n = 5). The maximal urethral closure pressure, the functional urethral length and the area under curve of the urethral pressure profile were measured. The mean maximal urethral closure pressure at the beginning of the experiment was 32 cmH(2) O, and the mean functional urethral length was 4.88 cm. The assessment at day 28 showed a reduction of the maximal urethral closure pressure (50% of the control, P > 0.05), the functional urethral length (52.5% of the control, P < 0.05) and the area under curve (52% of the control, P < 0.05) in injured pigs. Histologically, a fibrosis of the sphincter was detected without rupture of the muscle layer in all the samples. The proposed porcine model can be used to obtain intrinsic sphincter deficiency-like urodynamic findings without rupturing the sphincter. This methodology can be applied to investigate therapies for intrinsic sphincter deficiency.International Journal of Urology 05/2012; 19(7):676-82. · 1.75 Impact Factor -
Article: Protective effect of sodium ascorbate on efficacy of intramuscular transplantation of autologous muscle-derived cells.
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ABSTRACT: The possible reason for elimination of myogenic cells after transplantation is inflammation at the injection site associated with oxidative stress. The aim of this study was to determine whether preconditioning of muscle-derived cells with an antioxidant, sodium ascorbate, can influence the fate of transplanted cells. Autologous transplantation of muscle-derived cells was performed in rabbits. Isolated cells were identified, lipofected with β-galactosidase, preincubated or not with sodium ascorbate, and injected intramuscularly. Two weeks after autologous transplantation in the edge of a previous muscle defect, donor cells formed multinucleated young myotubes. Pretreatment of cells with sodium ascorbate before injection resulted in a significant increase of donor cells at the injection site 2 weeks after transfer. These results show that: (1) preincubation with antioxidant can increase the efficacy of myogenic cell transplantation; and (2) oxidative stress may play a role in elimination of cells after autologous transplantation.Muscle & Nerve 01/2012; 45(1):32-8. · 2.37 Impact Factor -
Article: Characterization of bone-marrow-derived rat mesenchymal stem cells depending on donor age.
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ABSTRACT: It is generally accepted that autologous transfers, as non-immunogenic, constitute the safest approach in cellular transplantations. However, this attitude is often associated with the need for isolation and extracorporeal propagation of cells derived from aged patients. Thus the knowledge about relationship between aging and the properties of MSCs (mesenchymal stem cells) is crucial in developing new clinical strategies. The aim of this study was to perform complex comparison of MSC derived from young and aged individuals, which included phenotype, proliferating rate, osteogenic and adipogenic potential and secretory activity. Evaluated populations were isolated from bone marrow of 3-month-old and 24-month-old rats. There was no significant difference in membrane antigen expression and PDT (population doubling time). Additionally, the adipogenic and osteogenic potential did not vary between studied populations. The reaction of MSCs to either mitogen [bFGF (basic fibroblas t growth factor)] or oxidative stress (H2O2) in vitro displayed a very similar pattern in both analysed populations. There was no difference in TGFβ1 (transforming growth factor β1) and VEGF (vascular endothelial growth factor) secretion measured by ELISA test and gene expression evaluated by real-time PCR. However, the expression of the gene for IL-1α (interleukin-1α) was 8-fold lower in oMSC (MSC isolated from old rats). These results indicate that aging individuals can be considered as candidates for autologous transplantation of bone-marrow-derived MSCs.Cell Biology International 05/2011; 35(10):1055-62. · 1.48 Impact Factor -
Article: Preincubation with bFGF but not sodium ascorbate improves efficiency of autologous transplantation of muscle-derived cells into urethral wall.
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ABSTRACT: Myogenic cell therapy is considered a new method to treat urinary incontinence. One of the crucial limitations in cellular transplantation is poor cell survival after cell transfer. Preconditioning of cells before transfer is a new approach in improving the efficacy of graft. In this study, autologous cell transplantation was performed on a rat model. Muscle-derived cells were isolated, identified with immunofluorescence and immunoblotting, preincubated or not with 10 ng/mL of basic fibroblast growth factor (bFGF) or 0.1 mM of sodium ascorbate for 24 hours, and transfected with the beta-galactosidase encoding gene using lipofectamine. Cell suspension was injected into the urethral wall. The tissue was collected 2 weeks after injection. The presence of transplanted cells was confirmed by X-Gal staining. Moreover, the effects of preincubation with either sodium ascorbate or bFGF 24 hours before transfer were evaluated. A semiqualitative analysis using chlorophenol-red-beta-d-galactopyranoside substrate demonstrated that preincubation with ascorbate had no effect on the number of transplanted cells but that bFGF significantly increased the amount of cells in the engrafted tissue by 46% (P < .05). Preconditioning of myogenic cells with bFGF should be seriously considered as a new tool for improving cell viability after cell transfer in the treatment of urinary incontinence.Urology 02/2009; 73(4):736-42. · 2.43 Impact Factor -
Article: [Myogenic stem cells--the new material for periurethral injections in the treatment of urinary incontinence].
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ABSTRACT: Urinary incontinence is currently considered to be not only medical, but also socio-economical problem. Periurethral injections are one of the treatment method, although substances used so far have been not effective enough. For last several years surveys are conducted for using myogenic cells as a new material for periurethral injections. It has been proven that undifferentiated muscle cells can be isolated in the amount sufficient for transplantation from small piece of skeletal muscle tissue. Myogenic cells survive and differentiate into muscle fibers when transplanted in the urethral sphincter. Indirect evidences suggest that injected cells can become innervated. Functional studies revealed that cellular transfer improve sphincter contractility and increase leak point pressure. In first clinical study in human medicine, where the effect was evaluated one year after surgery, 79% of patients was judged as cured. Apart from cells derived from muscle, also bone marrow-derived mesenchymal stem cells and cells seeded on biological scaffolds are considered for using in treatment of urinary incontinence.Przegla̧d lekarski 02/2008; 65(7-8):362-6. -
Article: Myogenic stem cells.
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ABSTRACT: Both skeletal muscle and bone marrow tissue contain myogenic stem cells. The population residing in muscles is heterogenic. Predominant in number are "typical" satellite cells - muscle progenitors migrating from somites during embryonic life. Another population is group of multipotent muscle stem cells which, at least in part, are derived from bone marrow. These cells are tracked by gradient of growth factors releasing from muscle during injury or exercise. Recruited bone marrow-derived cells gradually change their phenotype becoming muscle stem cells and eventually can attain satellite cell position and express Pax7 protein. Mesenchymal stem cells (MSC) isolated directly from bone marrow also display myogenic potential, although methods of induction of myogenic differentiation in vitro have not been optimized yet. Concerning efforts of exploiting myogenic stem cells in cell-mediated therapies it is important to understand the cause of impaired regenerative potential of aged muscle. Up to now, most of research data suggest that majority of age related changes in skeletal muscles are reversible, thus depending on extrinsic factors. However, irreversible intrinsic features of muscle stem cells are also taken into consideration.Folia Histochemica et Cytobiologica 02/2008; 46(4):401-12. · 0.81 Impact Factor -
Article: Sodium ascorbate and basic fibroblast growth factor protect muscle-derived cells from H2O2-induced oxidative stress.
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ABSTRACT: Engraftment of muscle-derived cells (MDCs) into the urethral sphincter may cure urinary incontinence. However, poor cell survival after injection prompted us to evaluate whether 24-h preincubation with sodium ascorbate (ASC) or basic fibroblast growth factor (bFGF) prior to cell transfer improves the survival of MDCs facing oxidative stress in vitro. We examined MDCs isolated from female rats for the presence of myogenic markers and for the ability to differentiate and respond to growth factors. Isolated cells were positive for desmin, M-cadherin, and myogenin. The fusion index exceeded 29%, and Akt kinase was phosphorylated at Ser473 residue upon exposure to insulin-like growth factor 1 (100 ng/ml). We then autologously transplanted MDCs transfected with lacZ marker gene into urethral wall of the rats; 2 wk later, the urethra and caudal wall of the urinary bladder were harvested from these animals. Serial cryosections revealed that transplanted cells formed multinuclear clusters at injection sites. In addition, we found that the viability of MDCs exposed to a cytotoxic concentration of H2O2 was higher after preincubation with 0.1 mM ASC (2.6-fold), 10 ng/ml bFGF (2.9-fold), or 20 ng/ml bFGF (3.5-fold) than that after exposure to H2O2 only. We conclude that preincubation with ASC or bFGF increases the resistance of MDCs to oxidative stress in vitro. Pretreatment with either agent might be used to enhance survival of MDCs after transplantation.Comparative medicine 01/2007; 56(6):493-501. · 1.05 Impact Factor -
Article: [Muscle cell transplantations: the ups and downs].
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ABSTRACT: Cell transplantation is believed to be an attractive technique among the various prospective methods of healing muscle wasting and other degenerative diseases. Muscle precursor cells can be obtained and cultured in vitro relatively easily, making possible a wide application of this method in the near future. A number of research efforts regarding cell transplantation for the recovery of dystrophic muscles and attempts to accelerate convalescence of disabled heart muscle are underway. There are also initiatives to use muscle cells in the repair of urinary incontinence. In the case of muscle dystrophy, very promising results were achieved in animal models, but the procedure has proved unreliable in clinical tests on humans. More convincing results were obtained from muscle cells transferred to myocardium. This procedure gave positive response in both animal models and clinical trials. However, there are still several obstacles to transplant muscle cells. First there is the poor viability of muscle cells after transfer. This results in sudden cell death, which occurs within a few hours after the cells are transferred to the recipient. A major concern recently is to develop procedures which will improve the efficacy of muscle cell transplantation. Growing interest is focused on autologous cell transplantation owing to the low immunogenicity of this kind of transfer. Moreover, numerous attempts are underway to suppress inflammation at the site of cell deposition or to search for subpopulations of cells which would result in a higher survival rate after transfer. Furthermore, genetic manipulations or preconditioning of muscle cells prior to transfer are often performed.Postępy Higieny i Medycyny Doświadczalnej (Advances in Hygiene and Experimental Medicine) 02/2005; 59:299-308.
Top co-authors
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Institutions
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2008–2009
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Medical University of Warsaw
- Klinika Immunologii, Transplantologii i Chorób Wewnętrznych
Warsaw, Masovian Voivodeship, Poland
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2007
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University of Warsaw
Warsaw, Masovian Voivodeship, Poland
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