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Publications (4)1.88 Total impact

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    ABSTRACT: To evaluate the role of Losartan in skin healing repair. One hundred and eleven male Wistar rats were distributed into four groups, at random. Group I (n=31) underwent a laparotomy to induce hypertension by stenosis of the left renal artery, and 48 hours later it received Losartan (10 mg/Kg) daily. Group II (n=30) went through the same procedure and received isotonic saline solution. Group III (n=30) underwent a simulated laparotomy. Group IV (n=20) to confirm the induced hypertension method. Ketamine and Xylazin anesthesia was used in every painful/stressful procedure. After 15 days, skin healing repair was studied by a dorsal midline skin incision and second layer-skin incision in groups I, II and III, that was sutured in one layer with 4-0 nylon suture. Samples of the dorsal wall scar were taken 4, 7 and 14 days after the last procedure, and sent to strength and displacement analysis and histological preparation. By the 4th and 7th days of the analysis, group II scars showed to be less resistant than group III scars (p<0,05). The total amount of collagen was higher in group III on the three studied periods. The percentage occupied by total collagen in the wound area, on the 14th day, was lower in group I, due to its lower percentage of type I collagen Scars treated with Losartan were initially less resistant and had a lower collagen deposition.
    Acta Cirurgica Brasileira 01/2006; 21(3):144-50. · 0.63 Impact Factor
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    ABSTRACT: The aging of the population has led to an increased number of interventions in elderly patients, with hypothyroidism, even at the subclinical level, being a common situation among these patients. The objective of the present study was to determine the influence of hypothyroidism on the healing process of colon anastomoses in aged rats. 96 male Wistar rats were used, 48 of them young and 48 old. Half the young animals and half the old ones were euthyroid and half were hypothyroid. Hypothyroidism was obtained by total thyroidectomy. Thirty days after surgery the animals were submitted to median laparotomy followed by transverse colotomy of the left colon with end-to-end anastomosis and laparorrhaphy. Six animals from each group were submitted to euthanasia by drawing lots on the 3rd, 7th and 14th postoperative day and submitted to macroscopic, resistance, and histopathological analysis of the anastomoses. No dehiscence was observed and the gain of resistance and the general histological evolution in terms of epithelialization and inflammatory reaction was similar in all groups. Analysis of total collagen content revealed that the anastomoses of young and old animals showed the same behavior on the 3rd day, although less collagen was observed in hypothyroid animals (YN x YH p = 0.0000; ON x OH p = 0.0000). This behavior persisted on the 7th and 14 day. Less collagen I was present in the anastomoses of hypothyroid animals on the 3 (YN x YH p = 0.0015; ON x OH p = 0.0000), 7th (YN x YH p = 0.0006; ON x OH p = 0.0001) and 14th day (YN x YH p = 0.0181; ON x OH p = 0.0057), the same occurring for collagen III on the 3rd (x YH p = 0.0007; ON x OH p = 0.0260), 7th (YN x YH p = 0.0160; ON x OH p = 0.2670) and 14th day (YN x YH p = 0.0000; ON x OH p = 0.0030). Analysis of the results permitted us to conclude that lower collagen concentration du to low synthesis and delayed scar maturation in the anastomoses occurred and was much more due to hypothyroidism than to aging.
    Acta Cirurgica Brasileira 02/2005; 20 Suppl 1:204-10. · 0.63 Impact Factor
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    Acta Cirurgica Brasileira - ACTA CIR BRAS. 01/2005; 20.
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    ABSTRACT: TURPOSE: The aging of the population has led to an increased number of interventions in elderly patients, with hypothyroidism, even at the subclinical level, being a common situation among these patients. The objective of the present study was to determine the influence of hypothyroidism on the healing process of colon anastomoses in aged rats. METHODS: 96 male Wistar rats were used, 48 of them young and 48 old. Half the young animals and half the old ones were euthyroid and half were hypothyroid. Hypothyroidism was obtained by total thyroidectomy. Thirty days after surgery the animals were submitted to median laparotomy followed by transverse colotomy of the left colon with end-to-end anastomosis and laparorrhaphy. Six animals from each group were submitted to euthanasia by drawing lots on the 3rd, 7th and 14th postoperative day and submitted to macroscopic, resistance, and histopathological analysis of the anastomoses. RESULTS: No dehiscence was observed and the gain of resistance and the general histological evolution in terms of epithelialization and inflammatory reaction was similar in all groups. Analysis of total collagen content revealed that the anastomoses of young and old animals showed the same behavior on the 3rd day, although less collagen was observed in hypothyroid animals (YN x YH p=0.0000; ON x OH p=0.0000). This behavior persisted on the 7th and 14th day. Less collagen I was present in the anastomoses of hypothyroid animals on the 3rd (YN x YH p=0.0015; ON x OH p=0.0000), 7th (YN x YH p=0.0006; ON x OH p=0.0001) and 14th day (YN x YH p=0.0181; ON x OH p=0.0057), the same occurring for collagen III on the 3rd (x YH p=0.0007; ON x OH p=0.0260), 7th (YN x YH p=0.0160; ON x OH p=0.2670) and 14th day (YN x YH p=0.0000; ON x OH p=0.0030). CONCLUSION: Analysis of the results permitted us to conclude that lower collagen concentration due to low synthesis and delayed scar maturation in the anastomoses occurred and was much more due to hypothyroidism than to aging.
    Acta Cirurgica Brasileira 12/2004; 20:113-119. · 0.63 Impact Factor