Xiangyang Lu

Chinese Academy of Agricultural Sciences, Peping, Beijing, China

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Publications (7)14.06 Total impact

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    ABSTRACT: The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA. Cloning of the NtTIR1 gene will enable us to further understand the molecular organization of the TIR1 and its possible function in the tobacco. Keywords Nicotiana tabacum –TIR1–RACE
    Russian Journal of Plant Physiology 01/2011; 58(1):149-156. · 0.62 Impact Factor
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    ABSTRACT: Cucumber (Cucumis sativus L.), which is a vegetable crop, has served as the model system for sex expression in flowering plants, and the inheritance of sex expression in cucumber is well documented. However, the genetics of subgynoecism expression in cucumber had rarely been described. In this study, we investigated the inheritance of subgynoecious traits in cucumber plants with the inbred cucumber lines of subgynoecious (C. sativus L. var sativus cv 97-17 and S-2-98) as the materials. Genetic analysis had showed the two subgynoecious inbred lines were controlled by one pair of recessive gene and one pair of incompletely dominant gene, which were designated presently as mod-F2 and Mod-F1, respectively. Furthermore, the mod-F2 and Mod-F1 loci, which enhance the intensity of femaleness, also inherited independently with F and M genes.
    Scientia Horticulturae - SCI HORT-AMSTERDAM. 01/2011; 127(3):464-467.
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    ABSTRACT: Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species (ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance.
    Transgenic Research 12/2010; 20(4):857-66. · 2.61 Impact Factor
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    ABSTRACT: Drought and high-salinity are the important constraints that severely affect plant development and crop yield worldwide. It has been established that ethylene response factor (ERF) proteins play important regulatory roles in plant response to abiotic and biotic stresses. Our previous researches have revealed that transgenic tobacco over-expressing TERF1 (encoding a tomato ERF protein) showed enhanced tolerance to abiotic stress. Here, we further investigate the function of TERF1 in transgenic rice. Compared with the wild-type plants, overexpression of TERF1 resulted in an increased tolerance to drought and high-salt in transgenic rice. And the enhanced tolerance may be associated with the accumulation of proline and the decrease of water loss. Furthermore, TERF1 can effectively regulate the expression of stress-related functional genes Lip5, Wcor413-l, OsPrx and OsABA2, as well as regulatory genes OsCDPK7, OsCDPK13 and OsCDPK19 under normal growth conditions. Our analyses of cis-acting elements show that there exist DRE/CRT and/or GCC-box existing in TERF1 targeted gene promoters. Our results revealed that ectopic expression of TERF1 in rice caused a series of molecular and physiological alterations and resulted in the transgenic rice with enhanced tolerance to abiotic stress, indicating that TERF1 might have similar regulatory roles in response to abiotic stress in tobacco and rice.
    Plant Cell Reports 10/2008; 27(11):1787-95. · 2.51 Impact Factor
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    ABSTRACT: Cucumber (Cucumis sativus L.) has served as the model system for sex expression in flowering plants and its sex type is predominantly controlled by two genetic loci, F and M. Ethylene is the major plant hormone that regulates sex expression in cucumber. The current model predicts that ethylene serves as both a promoter of femaleness via the F locus and an inhibitor of the male sex via the M locus. In support of this model, genetic, genomic, and transcript analyses indicate that the F gene encodes a key enzyme in ethylene biosynthesis. In this study, we discovered that the M locus co-segregates with an ETHYLENE-INSENSITIVE3 (EIN3)-like genomic sequence in an F2 population of 96 individuals. This genetic association agrees with the prediction that the M locus is involved in ethylene signaling, thus providing another line of evidence in favor of the model. In addition, we generated an amplified fragment length polymorphism (AFLP) map of the M locus, which was delimited into a genetic interval of 2.5 cM. The genetic association and the local map will assist the molecular isolation of the M gene using the combination of positional cloning and candidate gene approach.
    Theoretical and Applied Genetics 10/2008; 117(6):927-33. · 3.66 Impact Factor
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    ABSTRACT: Ethylene responsive factors (ERFs) are important plant-specific transcription factors, some of which have been demonstrated to interact with the ethylene-responsive GCC box and the dehydration-responsive element (DRE); however, data on the roles of ERF proteins in connection with various signaling pathways are limited. In this research, we used the GCC box, an essential cis-acting element responsive to ethylene and methyl jasmonate (MeJA), as bait in a yeast one-hybrid system to isolate transcription factors from tomato (Lycopersicon esculentum Mill.). One of the cDNAs, which was designated Jasmonate and Ethylene Response Factor 1 (JERF1), encodes an ERF protein, containing a conserved ERF DNA-binding motif and functioning as a transcriptional activator in yeast through targeting to the nucleus in onion (Allium cepa L.) epidermal cells. Biochemical analysis revealed that JERF1 bound not only to the GCC box but also to the DRE sequence. Expression of the JERF1 gene in tomato was induced by ethylene, MeJA, abscisic acid (ABA) and salt treatment, indicating that JERF1 might act as a connector among different signal transduction pathways. Further research with transgenic JERF1 tobacco (Nicotiana tabacum L.) plants indicated that overexpressing JERF1 activated expression of GCC box-containing genes such as osmotin, GLA, Prb-1b and CHN50 under normal growth conditions, and subsequently resulted in enhanced tolerance to salt stress, suggesting that JERF1 modulates osmotic tolerance by activation of downstream gene expression through interaction with the GCC box or DRE.
    Planta 01/2005; 220(2):262-70. · 3.35 Impact Factor
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    ABSTRACT: The expression ofArabidopsis PDF1.2 gene is regulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element responsive to ET, however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element inPDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combiningAgrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression ofPDF1.2 was confirmed by using the upstream −1.86 kb fragment ofPDF1.2 gene. Secondly, the upstream −300–−243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the −300–−243 bp fragment of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box inPDF1.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4×GCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results indicate that the GCC box inPDF1.2 is an essential and sufficient element to confer MeJA induction. KeywordsAgroinfiltation-GCC box-MeJA-PDF1.2 promoter- cis-acting element
    Chinese Science Bulletin 49(23):2476-2480. · 1.32 Impact Factor

Publication Stats

120 Citations
14.06 Total Impact Points

Institutions

  • 2005–2010
    • Chinese Academy of Agricultural Sciences
      • Institute of Vegetables and Flowers
      Peping, Beijing, China
    • Hunan Agricultural University
      Ch’ang-sha-chen, Jiangsu Sheng, China