Klaus Witter

Publications (2)5.67 Total impact

• Article: Identification of a new HLA-B allele (B*1576) by haplotype specific extraction.

  Johannes Dapprich, Klaus Witter, Harrison W Gabel, Nancy B Murphy, Ekkehard D Albert
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  ABSTRACT: Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the diploid sample into its haploid components. The sample was then re-typed using standard SBT, revealing a new human leukocyte antigen (HLA) allele, since named B*1576. HSE used in conjunction with standard SBT is a convenient and simple tool for resolving ambiguous and novel allele combinations without the need for amplification or subcloning.
  Human Immunology 06/2007; 68(5):418-21. · 2.84 Impact Factor
• Article: HLA-DP polymorphism in Venezuelan Amerindians.

  Ketevan Gendzekhadze, Fidias Herrera, Silvia Montagnani, Omar Balbas, Klaus Witter, Ekkehard Albert, Zulay Layrisse
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  ABSTRACT: Three different Venezuelan Amerindian tribes were studied for human leukocyte antigen (HLA)-DPA1 and DPB1 allelic variability using polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) and sequence-based typing in a selected group of samples. These tribes are geographically (two from the Perija Mountain range and one from the Orinoco Delta) and linguistically distinct: the Bari (from Campo Rosario and Saymaidoyi villages) and the Warao have been classified within the Chibcha linguistic family, whereas the Yucpa (from the Aroy, Marewa, and Peraya villages) are Carib speaking. Venezuelan Indians, like other Native American tribes, show a markedly reduced number of different HLA-DP alleles (range, 2-7) and haplotypes (range, 4-11) in comparison with neighboring Venezuelan mestizo and other non-Indian populations. Some HLA-DPB1 (*0402 and *1401) alleles characteristic for all Amerindian tribes are present also in these populations. Despite general similarities, each tribe and, in some cases, some subtribes show their own pattern of allele and haplotype distribution apparently more as a result of linguistic than to geographic variation.
  Human Immunology 01/2005; 65(12):1483-8. · 2.84 Impact Factor