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Yun-fei Wang,
Hua-fa Que,
Jie-nan Xu,
Han-jun Tang,
Huan-yu Xiang,
Xiao-dong Liu,
Zhen Zhang,
Jie Xing,
Liang Shen, Wei Shan,
An-min Liu,
Lian-yin Qiu,
Da-yi Deng,
Dan Gao
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ABSTRACT: Chronic ulcer of the lower extremities amounts for a grave and serious problem for public health. Western medicine focuses on controlling infection, improving blood circulation, surgical debridement, skin grafting, etc, but there are bottlenecks in the treatment. Traditional Chinese medicine (TCM) has a long history and a legacy of sound clinical efficacy in this area. TCM has developed a unique, effective external theory, and a large number of topical prescriptions and external technology. Through this research, a safe and effective treatment protocol of TCM for chronic ulcer of the lower extremities can be formed. To this end, during China's "Eleventh Five-Year" Plan, special research committees and projects on TCM external treatments and external technologies were established. This study on ulcer of the lower extremities constitutes one of the major research topics.
Clinical information of patients with chronic ulcer of the lower extremities will be first collected in a large, multicenter, epidemiological survey. Concurrently, a large multicenter, randomized, parallel-group, prospective study will be launched based on evidence-based medical principles to evaluate the efficacy and safety of external methods for removing carrion, dissolving stasis, reinforcing deficiency and promoting tissue regeneration. The evaluated indexes will include the wound healing percentage for primary outcome, wound healing time, wound healing rate, time and rate of removal of necrotic tissue, and TCM syndromes for secondary outcomes and routine blood test, routine urine test, liver and kidney function, blood mercury content and finally urine mercury content for adverse events.
In this trial, the authors will evaluate the efficacy and safety of external methods for removing carrion, dissolving stasis, reinforcing deficiency and promoting tissue regeneration in cases of chronic ulcer of the lower extremities for standardizing external therapy of TCM for treatment of this condition, and establishing the clinical assessment system for TCM.
The research program was registered in the Chinese Clinical Trial Registry in both English and Chinese in June 2011. Registration number: ChiCTR-TRC-11001365.
Journal of Chinese Integrative Medicine 02/2012; 10(2):166-75.
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Journal of Chinese Integrative Medicine 11/2008; 6(10):1065-7.
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06/2006;
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ABSTRACT: An enzymatic microreactor has been fabricated based on the poly(methyl methacrylate) (PMMA) microchchip surface-modified with zeolite nanoparticles. By introducing the silanol functional groups, the surface of PMMA microchannel has been successfully modified with silicalite-1 nanoparticle for the first time due to its large external surface area and high dispersibility in solutions. Trypsin can be stably immobilized in the microchannel to form a bioreactor using silica sol-gel matrix. The immobilization of enzyme can be realized with a stable gel network through a silicon-oxygen-silicon bridge via tethering to those silanol groups, which has been investigated by scanning electron microscopy and microchip capillary electrophoresis with laser-induced fluorescence detection. The maximum proteolytic rate constant of the immobilized trypsin is measured to be about 6.6 mM s(-1). Using matrix assisted laser desorption and ionization time-of-flight mass spectrometry, the proposed microreactor provides an efficient digestion of cytochrome c and bovine serum albumin at a fast flow rate of 4.0 microL min(-1), which affords a very short reaction time of less than 5 s.
Lab on a Chip 05/2006; 6(4):534-9. · 5.67 Impact Factor
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Angewandte Chemie International Edition 04/2006; 45(13):2088-90. · 13.45 Impact Factor
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Angewandte Chemie 02/2006; 118(13):2142 - 2144.
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Journal of Chinese Integrative Medicine 06/2005; 3(3):235-7.
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ABSTRACT: Lotus-leaf-like silica flakes with a three-dimensionally (3D) connected nanoporous structure and controllable thickness have been facilely synthesized; the flakes produced exhibited superior performance in adsorbing enzymes to their microspheric analogues.
Chemical Communications 05/2005; · 6.17 Impact Factor
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Angewandte Chemie International Edition 02/2005; 44(4):615-7. · 13.45 Impact Factor
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ABSTRACT: Metal-ion-immobilized zeolite nanoparticles have been applied for the first time to isolate phosphopeptides from tryptic beta-casein digest; the phosphopeptides enriched on the modified zeolite nanoparticles could be effectively identified by MALDI-TOF-MS/MS.
Chemical Communications 01/2005; · 6.17 Impact Factor
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ABSTRACT: A novel catalyst of silver nanoparticles over a zeolite film-coated copper grid (SZFC) has been fabricated via an in situ electrolytic method; it exhibited high catalytic activity and selectivity at a relatively low temperature for the partial oxidation of 1,2-propylene glycol to methyl glyoxal.
Chemical Communications 01/2005; · 6.17 Impact Factor
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Wei SHAN
Zhong Xi Yi Jie He Xue Bao. 01/2005;
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Angewandte Chemie International Edition 12/2004; 44(4):615 - 617. · 13.45 Impact Factor
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Journal of Chinese Integrative Medicine 02/2004; 2(1):63-4.
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Chemistry Letters - CHEM LETT. 01/2004; 33(3):270-271.
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Chemistry Letters - CHEM LETT. 01/2004; 33(10):1248-1249.
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ABSTRACT: Thin zeolite films with thicknesses from several hundred nanometers to a few micrometers have been fabricated through electrophoretic deposition (EPD) in acetylacetone (Acac) and/or isopropyl alcohol (IPA). Various types of nanosized zeolites with different Si/Al ratios ranging from 1.6 (zeolite LTA) to nearly infinity (silicalite-1) were applied to prepare the EPD suspensions and showed different behaviors in EPD. The EPD rate of zeolite particles in non-aqueous medium was much higher than that in aqueous system mainly due to the high voltage applied, and consequently the whole process was usually completed in a few seconds. It was found that the Al/(Si + Al) ratios of zeolites and the chemical/physical properties of the EPD medium were two crucial factors which affected the thickness and density of the zeolite films prepared by EPD. The zeolite films fabricated through EPD could be effectively densified by secondary growth to prepare compact zeolite membranes.
Microporous and Mesoporous Materials.
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ABSTRACT: The selective oxidation of a series of alcohols to their corresponding carbonyl products was carried out over a rationally designed in situ electrolytic nano-silver/zeolite film/copper grid (SZF) catalyst, which was prepared by a combination of the seed-film method for the fabrication of an ultrathin zeolite film and the in situ electrolytic process for the formation of highly dispersed silver nanoparticles. At a relatively low reaction temperature (ca. 320 °C), the SZF catalyst with highly dispersed in situ electrolytic silver nanoparticles exhibited much higher activity for the oxidation of mono-alcohols and higher selectivity for ketonic aldehyde in the oxidation of di-alcohols than the conventional bulk electrolytic silver catalyst. On the basis of the combination of diffuse reflectance ultraviolet visible spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and thermoanalysis, the remarkably high activity and selectivity of the SZF catalyst was attributed to the highly dispersed silver nanoparticles, which were stabilized by the zeolite film against sintering, and, accordingly, a large amount of Ag+ ions and clusters existed in the silver nanoparticles. The improvements of the catalytic performance of the SZF catalyst in a wide application extension will bring new concerns in both theoretical and applied fields.
Journal of Catalysis.
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ABSTRACT: Various nanozeolites microspheres with uniform diameters from 3 to 8 µm were prepared by a polymerization-induced colloid aggregation method. The obtained nanozeolite microspheres possess a regularly spherical morphology and a large and adjustable secondary pore architecture that are believed to be useful in the separation of biomolecules. Moreover, the nanozeolite microspheres well retain the properties of the original colloidal nanozeolites and exhibit similar advantages to those of zeolite nanoparticles in protein adsorption. More importantly, the large secondary pore size (15-70 nm) of nanozeolite microspheres has been proven to have little limiting effect on the adsorption rate of various proteins, and a saturated adsorption is achieved even within 10 min, which may also facilitate microspheres' applications in catalysis involving diffusion limitations.
