Hae-Joon Park

Union Corporation, South Korea, Sŏul, Seoul, South Korea

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Publications (3)43.78 Total impact

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    ABSTRACT: The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques: nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/μl). The sensitivity threshold was 0.2 parasites/μl and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas.
    Acta tropica 06/2011; 120(1-2):40-5. DOI:10.1016/j.actatropica.2011.05.006 · 2.27 Impact Factor
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    ABSTRACT: We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast--the only other eukaryote for which a comprehensive knockout library exists--revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
    Nature Biotechnology 06/2010; 28(6):617-23. DOI:10.1038/nbt.1628 · 41.51 Impact Factor
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    ABSTRACT: Background: Genetic polymorphisms in VKORC1 (vitamin K epoxide reductase complex subunit 1) and CYP2C9 (cytochrome P450 2C9) genes are the most important genetic factors related to dose adjustment of oral anticoagulant warfarin. U.S. FDA and NACB recommend those genetic testing along with the administration of warfarin. Fast and reliable test method is essential for prompt dose adjustment of warfarin to prevent serious hemorrhagic or thrombotic complications. Authors developed a real-time PCR-based method for VKORC1 and CYP2C9 genotyping. Methods: Single nucleotide polymorphism (SNP) genotyping methods were developed under the real-time PCR platform using allele specific primers and 5' nuclease probes. Four well-known SNPs for VKORC1 gene (g.3673G>A, g.6484C>T, g.6853G>C, and g.9041G>A) and two SNPs for CYP2C9 gene (c.430C>T and c.1075A>C) were genotyped based on the differences in Ct (cycle threshold) in the real-time PCR system. Each reaction was conformed by PCR-direct sequencing method. Also multiplexing reaction was applied to reduce the number of wells and internal positive control (IPC) reaction was added to all the wells to guarantee successful reaction in each well. Results: The validity of amplification reactions was assessed based on the difference in Ct (DCt) for the reactions targeting two different alleles at each SNP site. For each SNP site, homozygote specimens showed DCt greater than 3.0 and heterozygote specimens showed DCt smaller than 1.0. Also no interference was observed in the multiplexed PCR environment and all the IPC reactions were successfully completed. All these results were concordant with those confirmed by PCR-direct sequencing method. Overall turnaround time was around 2 hours and 12 genomic DNA specimens could be analyzed simultaneously. Conclusion: We developed a genotyping kit for warfarin dosing using real-time PCR based allele-specific primers and probes, which has rapid turnaround time and consistent results with those by direct sequencing method. This method is expected to be practically applied in the clinical practice for the warfarin dose adjustment.
    9th International International society for the study of xenobiotics Meeting;