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Sang-Wook Kim,
Suck-Chei Choi,
Eun-Young Choi,
Kyoung-Suk Kim,
Jae-Min Oh,
Hyun-Ju Lee,
Hyun-Mee Oh,
Soonhag Kim,
Berm-Seok Oh,
Ku-Chan Kimm,
Moo-Hyung Lee,
Geom-Seog Seo,
Tae-Hyeon Kim,
Hyun-Cheol Oh,
Won-Hong Woo, Youn-Seok Kim,
Hyun-Ock Pae,
Do-Sim Park,
Hun-Taeg Chung,
Chang-Duk Jun
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ABSTRACT: Certain irinoid-producing plants have been used as herbal anti-inflammatory remedies. Here we evaluated whether catalposide (CATP), a single compound isolated from irinoid-producing plant Catalpa ovata, has a potential for preventing or ameliorating diseases characterized by mucosal inflammation. Preliminary microarray-based gene expression test revealed that CATP, which alone did not significantly affect expression of any of the >8,000 genes analyzed, attenuated the expression of tumor necrosis factor-alpha (TNF-alpha)-induced proinflammatory genes including interleukin-8 (IL-8) in human intestinal epithelial HT-29 cells. Down-regulation of IL-8 mRNA accumulation was also reflected by the decreased IL-8 secretion in CATP-treated HT-29 cells. The signal transduction study revealed that CATP significantly attenuates TNF-alpha-mediated p38 and extracellular signal-regulated kinase (ERK) phosphorylation. Further, CATP reduced NF-kappaB-mediated transcriptional activation as well as Ikappa-Balpha degradation. To establish the in vivo relevance of these findings, we examined whether CATP could affect intestinal inflammation in vivo using the mouse model of trinitrobenzene sulfonic acid (TNBS)-induced inflammatory colitis. Intrarectal administration of CATP dramatically reduced the weight loss, colonic damage, and mucosal ulceration that characterize TNBS colitis. Moreover, CATP suppressed the expression of TNF-alpha, interleukin-1beta, and intercellular adhesion molecule-1 along with the inhibition of NF-kappa B p65 translocation into nucleus in TNBS colitis. Collectively, current results demonstrate that CATP may be an effective agent for the treatment of diseases characterized by mucosal inflammation.
Inflammatory Bowel Diseases 09/2004; 10(5):564-72. · 4.86 Impact Factor
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ABSTRACT: Grid computing promises the ability to share geographically and organizationally distributed resources to increase effective computational power and resource utilization. However, for the Grid computing to be successful, it is very important to provide middleware services that assist Grid users to easily interact with Grid environments. In this paper, we have designed and implemented a new general-purpose OGSI-compliant Grid resource broker service to hide the underlying complexity of the Grid resources from Grid users and to meet not only Grid user requirements but also resource owner policies. It focuses on discovering and scheduling dynamic resources scattered across multiple organizations. Furthermore, it can be integrated with various scheduling services. We also present experimental results and demonstrate the effectiveness of our Grid broker service.
Cluster Computing and the Grid, 2004. CCGrid 2004. IEEE International Symposium on; 05/2004
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4th IEEE/ACM International Symposium on Cluster Computing and the Grid (CCGrid 2004), April 19-22, 2004, Chicago, Illinois, USA; 01/2004
