Roberta Serra

Università degli Studi di Sassari, Sassari, Sardinia, Italy

Are you Roberta Serra?

Claim your profile

Publications (1)1.72 Total impact

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Neopterin, a pyrazinopyrimidine compound, serves as a marker of cellular immune system activation, and it can be used as a prognostic predictor for certain types of diseases. We propose a new simple HPLC method to measure serum neopterin with highly sensitive fluorimetric detection. After TCA serum protein precipitation, the supernatant was diluted five times, injected into a C18 reversed-phase column and eluted at a flow rate of 1.5 mL/min by an isocratic water-acetonitrile (99:1) mobile phase. The natural fluorescence of the molecule was detected at excitation wavelength 353 nm and emission 438 nm. In these conditions the neopterin retention time was about 4 min. Our proposed method was compared with a validated chromatographic separation, and the obtained data of the serum neopterin from 35 healthy volunteers were analysed by Passing-Bablok regression and Bland-Altman test. Neopterin measurement in healthy subjects was also employed to investigate on its potential relationships with plasma thiols levels.
    Biomedical Chromatography 07/2004; 18(6). DOI:10.1002/bmc.325 · 1.72 Impact Factor