Gert Pflugfelder

Publications (2)8.01 Total impact

• Source

  Available from: David Gubb

  Article: The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.

  Edward Ryder, Michael Ashburner, Rosa Bautista-Llacer, Jenny Drummond, Jane Webster, Glynnis Johnson, Terri Morley, Yuk Sang Chan, Fiona Blows, Darin Coulson, [......], Gert Pflugfelder, Christian Dorner, Bernard Mechler, Heide Schenkel, Joachim Marhold, Florenci Serras, Montserrat Corominas, Adrià Punset, John Roote, Steven Russell
  [show abstract] [hide abstract]
  ABSTRACT: We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.
  Genetics 10/2007; 177(1):615-29. · 4.01 Impact Factor
• Source

  Available from: David Gubb

  Article: The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.

  Edward Ryder, Fiona Blows, Michael Ashburner, Rosa Bautista-Llacer, Darin Coulson, Jenny Drummond, Jane Webster, David Gubb, Nicola Gunton, Glynnis Johnson, [......], Jean Antoine Lepesant, Gert Pflugfelder, Martin Heisenberg, Bernard Mechler, Florenci Serras, Montserrat Corominas, Stephan Schneuwly, Thomas Preat, John Roote, Steven Russell
  [show abstract] [hide abstract]
  ABSTRACT: We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.
  Genetics 07/2004; 167(2):797-813. · 4.01 Impact Factor