Hélène Budzinski

Publications (2)3.05 Total impact

• Article: Production of highly 13C-labeled polyphenols in Vitis vinifera cell bioreactor cultures.

  Virginie Aumont, Fabienne Larronde, Tristan Richard, Hélène Budzinski, Alain Decendit, Gérard Deffieux, Stéphanie Krisa, Jean-Michel Mérillon
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  ABSTRACT: The use of Vitis vinifera cells grown in a 2 l-stirred tank bioreactor for producing isotopically 13C-labeled phenolic substances is presented. Several culture parameters were optimized to achieve characteristics of growth and polyphenol metabolism similar to that recorded in shake flasks. Administration of [1-13C]L-phenylalanine (3 mM) to grape cell suspension cultures led to the production of 13C-labeled stilbenes (trans- and cis-piceids), catechins (catechin and epicatechin) and anthocyanins (delphinidin-, cyanidin-, petunidin-, peonidin- and malvidin-3-O-beta-glucosides). Incorporation of [1-13C]L-phenylalanine into polyphenols was measured by means of 13C satellites in the proton NMR spectrum and EA-IRMS. The enrichment of labeling obtained for all the compounds (between 40 and 65%) is sufficient to investigate their absorption and metabolism in humans.
  Journal of Biotechnology 05/2004; 109(3):287-94. · 3.05 Impact Factor
• Article: Stilbene Production by Vitis vinifera Cell Suspension Cultures: Methyl Jasmonate Induction and 13C Biolabeling

  Stéphanie Krisa, Fabienne Larronde, Hélène Budzinski, Alain Decendit, Gérard Deffieux, Jean-Michel Mérillon
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  ABSTRACT: Vitis vinifera cells grown in vitro respond to methyl jasmonate (MeJa) with enhancement of phytoalexin accumulation. MeJa added during the first half of the exponential growth phase increased the intracellular accumulation of stilbenes, mainly (Z)- and (E)-piceid (3,5,4‘-trihydroxystilbene-3-O-β-glucoside), in strains grown in either maintenance medium or polyphenol inductive medium. Plant cell cultures were used to produce isotopically (13C) labeled stilbenes by addition of 13C phenylalanine at different time intervals and in various concentrations. Incorporation of 13C phenylalanine into stilbenes was measured by EA-IRMS, and three successive 2-mM phenylalanine additions provided the best result (66%). This allowed production of 13C labeled Vitis vinifera phenolic compounds for investigation of their absorption in humans.
  11/1999;