Publications (5)15.14 Total impact
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Dataset: 2005 Martin W et al
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Article: Comparative genomic analyses in Asparagus.
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ABSTRACT: Garden asparagus (Asparagus officinalis L.) belongs to the monocot family Asparagaceae in the order Asparagales. Onion (Allium cepa L.) and Asparagus officinalis are 2 of the most economically important plants of the core Asparagales, a well supported monophyletic group within the Asparagales. Coding regions in onion have lower GC contents than the grasses. We compared the GC content of 3374 unique expressed sequence tags (ESTs) from A. officinalis with Lycoris longituba and onion (both members of the core Asparagales), Acorus americanus (sister to all other monocots), the grasses, and Arabidopsis. Although ESTs in A. officinalis and Acorus had a higher average GC content than Arabidopsis, Lycoris, and onion, all were clearly lower than the grasses. The Asparagaceae have the smallest nuclear genomes among all plants in the core Asparagales, which typically have huge genomes. Within the Asparagaceae, European Asparagus species have approximately twice the nuclear DNA of that of southern African Asparagus species. We cloned and sequenced 20 genomic amplicons from European A. officinalis and the southern African species Asparagus plumosus and observed no clear evidence for a recent genome doubling in A. officinalis relative to A. plumosus. These results indicate that members of the genus Asparagus with smaller genomes may be useful genomic models for plants in the core Asparagales.Genome 01/2006; 48(6):1052-60. · 1.65 Impact Factor -
Article: Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity.
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ABSTRACT: The Poales (which include the grasses) and Asparagales [which include onion (Allium cepa L.) and other Allium species] are the two most economically important monocot orders. Enormous genomic resources have been developed for the grasses; however, their applicability to other major monocot groups, such as the Asparagales, is unclear. Expressed sequence tags (ESTs) from onion that showed significant similarities (80% similarity over at least 70% of the sequence) to single positions in the rice genome were selected. One hundred new genetic markers developed from these ESTs were added to the intraspecific map derived from the BYG15-23xAC43 segregating family, producing 14 linkage groups encompassing 1,907 cM at LOD 4. Onion linkage groups were assigned to chromosomes using alien addition lines of Allium fistulosum L. carrying single onion chromosomes. Visual comparisons of genetic linkage in onion with physical linkage in rice revealed scant colinearity; however, short regions of colinearity could be identified. Our results demonstrate that the grasses may not be appropriate genomic models for other major monocot groups such as the Asparagales; this will make it necessary to develop genomic resources for these important plants.Molecular and General Genetics 11/2005; 274(3):197-204. · 2.63 Impact Factor -
Article: A unique set of 11,008 onion expressed sequence tags reveals expressed sequence and genomic differences between the monocot orders Asparagales and Poales.
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ABSTRACT: Enormous genomic resources have been developed for plants in the monocot order Poales; however, it is not clear how representative the Poales are for the monocots as a whole. The Asparagales are a monophyletic order sister to the lineage carrying the Poales and possess economically important plants such as asparagus, garlic, and onion. To assess the genomic differences between the Asparagales and Poales, we generated 11,008 unique ESTs from a normalized cDNA library of onion. Sequence analyses of these ESTs revealed microsatellite markers, single nucleotide polymorphisms, and homologs of transposable elements. Mean nucleotide similarity between rice and the Asparagales was 78% across coding regions. Expressed sequence and genomic comparisons revealed strong differences between the Asparagales and Poales for codon usage and mean GC content, GC distribution, and relative GC content at each codon position, indicating that genomic characteristics are not uniform across the monocots. The Asparagales were more similar to eudicots than to the Poales for these genomic characteristics.The Plant Cell 02/2004; 16(1):114-25. · 8.99 Impact Factor -
Article: A genetic study of unilateral incompatibility between diploid (1EBN) Mexican species Solanum pinnatisectum and S. cardiophyllum subsp. cardiophyllum
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ABSTRACT: Many angiosperms have developed mechanisms to prevent self pollination and inbreeding. The most widespread system of self incompatibility is gametophytic, in which pollen tube growth is inhibited in the style by the action of a single locus (S-locus). Similar inhibition of pollen tube growth can also be observed in interspecific crosses, where successful pollinations occur in only one direction, termed unilateral incompatibility. We observed unilateral incompatibility in crosses between Solanum pinnatisectum and S. cardiophyllum, and viable interspecific hybrid seed was generated only when S. pinnatisectum was the pistillate parent. Segregations in the backcross to S. cardiophyllum revealed two independently segregating putative loci controlling interspecific crossability. Both putative loci were inherited independently of the S-locus. Segregation ratios in the backcross to the S. pinnatisectum parent did not agree with those observed in the backcross to the S. cardiophyllum parent. These reciprocal backcross families revealed that unilateral incompatibility is not conditioned by a single locus, and is inherited independently of the S-locus. Segregation disparity between the two backcross families may be due to distorted transfer of specific alleles or the presence of different mechanisms controlling pistil and pollen recognition systems in the two species.Sexual Plant Reproduction 03/2002; 14(6):305-313. · 1.87 Impact Factor
Top co-authors
Top Journals
- Genome (1)
- Sexual Plant Reproduction (1)
- The Plant Cell (1)
- Molecular and General Genetics (1)
Institutions
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2004–2006
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Michigan State University
- Department of Horticulture
East Lansing, MI, USA
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2002
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United States Department of Agriculture
- Agricultural Research Service (ARS)
Washington, D. C., DC, USA
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