Leah G Doan

Publications of Leah G Doan

• Ricin: mechanism of toxicity, clinical manifestations, and vaccine development. A review.

  Authors: Leah G Doan

  Journal of toxicology. Clinical toxicology. 02/2004; 42(2):201-8.

  Ricin is one of the most potent plant toxins known, and the castor plant from which it is derived, Ricinus communis, is ubiquitous. The harvesting of castor beans exceeds one million tons annually,Ricin is one of the most potent plant toxins known, and the castor plant from which it is derived, Ricinus communis, is ubiquitous. The harvesting of castor beans exceeds one million tons annually, and ricin is easier to produce than either anthrax or botulinum. As a result, ricin is a convenient, potent, and available toxin for terrorist acts. This paper will review the mechanism of toxicity, major clinical manifestations, treatment, current methods of detection, and vaccine development.

• Rapid enumeration of respiratory viruses.

  Authors: Matthew M Ferris, Mark O McCabe, Leah G Doan, Kathy L Rowlen

  Analytical chemistry. 05/2002; 74(8):1849-56.

  Virus detection and enumeration has become increasingly important in fields ranging from medicine and biotechnology to environmental science. Although there are a wide variety of techniques that canVirus detection and enumeration has become increasingly important in fields ranging from medicine and biotechnology to environmental science. Although there are a wide variety of techniques that can be used to count viruses, there is demand for a rapid and more accurate means for virus enumeration. In this work, the performance of a flow cytometer that was designed and custom-built specifically for rapid detection of single viruses was evaluated. The instrument, designated a single nanometric particle enumerator (SNaPE), was characterized and calibrated using fluorescent polystyrene nanospheres. The reliability of the instrument with respect to virus enumeration was demonstrated for three medically relevant viruses, adenovirus-5, respiratory syncytial virus, and influenza A, treated with a fluorescent nucleotide stain. In each case, the SNaPE yielded a virus particle concentration consistent with, but slightly lower than, transmission electron microscopy (TEM) results, as expected. In addition, on the basis of calibration of signal intensity, the average peak height for a given virus was correlated with genome size, as expected. In contrast to time-consuming analyses such as TEM and plaque titers, SNaPE analysis of pure virus samples (including sample handling, data collection, and data processing) can be completed within 1 h.