ABSTRACT: RadA is involved in strand exchange reactions in homologous recombination which is a fundamental process in all organisms. Sulfolobus tokodaii, one of the archeabacteria, is an aerobic thermoacidophilic crenearchaeon which was isolated from hot springs. RadA from S. tokodaii (stRadA) is a heat resistance protein. The purified protein showed a single band on SDS-PAGE, and gel filtration analysis indicated that stRadA formed a multimeric structure. To reveal a mechanism of the homologous recombination at atomic resolution, stRadA was crystallized with DNA, and its crystals were obtained in 92 conditions by vapor-diffusion methods.
Nucleic Acids Symposium Series 02/2004;