Zhi-Qiang Du

Publications (4)2.34 Total impact

• Article: Single nucleotide polymorphisms in the chicken Lmbr1 gene are associated with chicken polydactyly.

  Yan Qun Huang, Xue Mei Deng, Zhi Qiang Du, Xiangpin Qiu, Xiaohui Du, Wen Chen, Mireille Morisson, Sophie Leroux, F Abel Ponce de Léon, Yang Da, Ning Li
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  ABSTRACT: Polydactyly is a common malformation of vertebrate limbs. Preaxial polydactyly (PPD) has been mapped in human, mouse and chicken to the syntenic region of human 7q36. Lmbr1 was thought as the critical candidate gene for human and mouse PPD. To understand the molecular mechanism underlying chicken polydactyly, we have cloned the open reading frame (ORF) of chicken Lmbr1, which contains 1467 nucleotides. Within this ORF, we found one short and one long splice forms. The short splice form has a complete deletion of exon 4. Six cSNPs were found in the chicken ORF, and two of these cSNPs, G797A and G1255A, lead to amino acid substitutions. However, G797A substitution had no significant association with polydactyly and the G1255A substitution had very low frequency in the population. The T1254C polymorphism in exon 13 was found to be strongly associated with polydactyly. Radiation hybrid mapping of a DNA fragment containing intron 13 of the chicken Lmbr1 assigned the gene to chromosome 2 between MCW071 (a marker within the EN2 gene) and ADL0270, a syntenic region to human 7q36.
  Gene 07/2006; 374:10-8. · 2.34 Impact Factor
• Article: [Comparison of denatured and non-denatured PAGE-silver-staining detection of PCR product of microsatellites].

  Lu-Jiang Qu, Xian-Yao Li, Zhi-Qiang Du, Long-Chao Zhang, Ning Yang
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  ABSTRACT: The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.
  Hereditas (Beijing) 08/2004; 26(4):522-4.
• Article: [Genetic diversity in Tibetan chicken].

  Zhi-Qiang Du, Lu-Jiang Qu, Xian-Yao Li, Xiao-Xiang Hu, Yin-Hua Huang, Ning Li, Ning Yang
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  ABSTRACT: Morphological traits and living habit of Tibetan chicken, which is an aboriginal chicken breed on plateau with its own characteristic populational genetic features, are in great common with the Red Jungle Fowl, the assumed ancestry of domestic chicken. To fully exploit this chicken resource, Multiplex PCR with semi-automated polyacrilamide gel electrophoresis (PAGE) using fluorescently labeled microsatellite primers was used to detect the polymorphism at 20 microsatellite loci. At the same time, we randomly test the individual morphology and performance. It showed that numbers of polymorphic alleles were 4 approximately 10, with mean value 7.25 per locus. Polymorphism Information Content (C(PI)) and Heterozygosity (H) had mean values 0.67 and 0.74, respectively. Macrochromosomes had relatively higher polymorphism than microchromosomes(P > 0.05). In all, high polymorphisms at microsatellite loci related to the uneven production performance and morphological discrepancy of population genetic characteristics in Tibetan chicken.
  Hereditas (Beijing) 04/2004; 26(2):167-71.
• Source

  Available from: chinagene.cn

  Article: [Preliminary analysis on China Agricultural University chicken resource population based on genomic scanning].

  Xiao-Xiang Hu, Yin-Hua Huang, Yu Gao, Zhi-Qiang Du, Ji-Dong Feng, Wei-Zhuo Xu, Xue-Mei Deng, Ying Zhang, Bao-Liang Fan, Zhi-Hui Zhao, Zhao-Liang Liu, Ning Yang, Ning-Yi Jin, Chang-Xin Wu, Ning Li
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  ABSTRACT: Combining the technique of multiplex-PCR and the fluorescent semi-automated detection, a large-scale genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families, within three generations. Fifty-five microsatellite markers were analyzed for this study. Those 55 microsatellite loci accorded with the characters of Mendelian co-inheritance. The heterozygosities ranged from zero to 0.89, with 72% of loci having a heterozygosity of more than 0.60. The polymorphism information content (PIC) ranged from 0 to 0.85, in which 70% of those loci had a PIC of more than 0.50 but their distribution varied in line A and line C. The allele frequency was significantly different between line A and line C at most loci (P < 0.01). At the same time, gene accordance inclination was found in line C. The Nei population resemble coefficient and standard genetic distance were 0.1002 and 0.8928.
  Acta Genetica Sinica 12/2003; 30(12):1101-6.