Cemaliye Boylu Akyerli

Publications (4)14.58 Total impact

• Article: Extremely skewed X-chromosome inactivation patterns in women with recurrent spontaneous abortion.

  Sevgi Bagislar, Isik Ustuner, Bora Cengiz, Feride Soylemez, Cemaliye Boylu Akyerli, Serdar Ceylaner, Gulay Ceylaner, Aynur Acar, Tayfun Ozcelik
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  ABSTRACT: The role of extremely skewed X-chromosome inactivation (XCI) has been questioned in the pathogenesis of recurrent spontaneous abortion (RSA) but the results obtained were conflicting. We therefore investigated the XCI patterns in peripheral blood DNA obtained from 80 patients who had RSA and 160 age-matched controls. Pregnancy history, age, karyotype, and disease information was collected from all subjects. The methylation status of a highly polymorphic cytosine-adenine-guanine repeat in the androgen-receptor (AR) gene was determined by use of methylation-sensitive restriction enzyme HpaII and polymerase chain reaction. Skewed XCI (> 85% skewing) was observed in 13 of the 62 patients informative for the AR polymorphism (20.9%), and eight of the 124 informative controls (6.4%) (P = 0.0069; chi2 test). More importantly, extremely skewed XCI, defined as > 90% inactivation of one allele, was present in 11 (17.7%) patients, and in only two controls (P = 0.0002; chi2 test). These results support the interpretation that disturbances in XCI mosaicism may be involved in the pathogenesis of RSA.
  Australian and New Zealand Journal of Obstetrics and Gynaecology 10/2006; 46(5):384-7. · 1.24 Impact Factor
• Article: Skewed X chromosome inactivation in blood cells of women with scleroderma.

  Zeynep Ozbalkan, Sevgi Bagişlar, Sedat Kiraz, Cemaliye Boylu Akyerli, Hüseyin T E Ozer, Sule Yavuz, A Merih Birlik, Meral Calgüneri, Tayfun Ozçelik
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  ABSTRACT: Scleroderma (SSc) is an autoimmune disease of unknown etiology. The disease is 3-8 times more frequent in women than in men. The role of X chromosome inactivation (XCI) in the predisposition of women to autoimmunity has been questioned. Until now this has not been illustrated experimentally. This study was undertaken to test the hypothesis that disturbances in XCI mosaicism may be involved in the pathogenesis of the disease in female patients with SSc. Seventy female SSc patients and 160 female controls were analyzed for the androgen receptor locus by the Hpa II/polymerase chain reaction assay to assess XCI patterns in DNA extracted from peripheral blood cells. Furthermore, skin biopsy samples were obtained from 5 patients whose blood revealed an extremely skewed pattern of XCI, and the analysis repeated. Since microchimerism in SSc was reported, Y chromosome sequences were investigated in all samples. Skewed XCI was observed in DNA from peripheral blood cells in 35 of 55 informative patients (64%), as compared with 10 of 124 informative controls (8%) (P < 0.0001). Extreme skewing was present in 27 of the patient group (49%), as compared with only 3 of the controls (2.4%) (P < 0.0001). However, XCI was random in all skin biopsy samples. The potential contribution of microchimerism to the random XCI pattern is highly unlikely based on the medical histories of the patients. Skewed XCI mosaicism may play a significant role in the pathogenesis of SSc.
  Arthritis & Rheumatism 06/2005; 52(5):1564-70. · 7.87 Impact Factor
• Article: Expression of IFITM1 in chronic myeloid leukemia patients.

  Cemaliye Boylu Akyerli, Meral Beksac, Michelle Holko, Mathias Frevel, Klara Dalva, Uğur Ozbek, Ender Soydan, Muhit Ozcan, Gülsüm Ozet, Osman Ilhan, Günhan Gürman, Hamdi Akan, Bryan R G Williams, Tayfun Ozçelik
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  ABSTRACT: We investigated the peripheral blood gene expression profile of interferon induced transmembrane protein 1 (IFITM1) in sixty chronic myeloid leukemia (CML) patients classified according to new prognostic score (NPS). IFITM1 is a component of a multimeric complex involved in the trunsduction of antiproliferative and cell adhesion signals. Expression level of IFITM1 was found significantly different between the high- and low-risk groups (P = 9.7976 x 10(-11)) by real-time reverse transcription polymerase chain reaction (RT-PCR). Higher IFITM1 expression correlated with improved survival (P = 0.01). These results indicate that IFITM1 expression profiling could be used for molecular classification of CML, which may also predict survival.
  Leukemia Research 04/2005; 29(3):283-6. · 2.92 Impact Factor
• Article: Analysis of MYH Tyr165Cys and Gly382Asp variants in childhood leukemias.

  Cemaliye Boylu Akyerli, Uğur Ozbek, Müge Aydin-Sayitoğlu, Sema Sirma, Tayfun Ozçelik
  Journal of Cancer Research and Clinical Oncology 11/2003; 129(10):604-5. · 2.56 Impact Factor