Congjing Feng

Publications (6)10.52 Total impact

• Article: Cloning and characterization of a novel splice variant of human U2AF1L3 gene†Full Length Research Paper

  Fei Chen, Chaoneng Ji, Tonghai Dou, Nan Zheng, Rui Qiu, Jing Peng, Weiqun Fang, Congjing Feng, Yi Xie, Yumin Mao
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  ABSTRACT: Pre-mRNA splicing allows individual genes to produce multiple protein isoforms with diverse functions. Recognition of functional splice sites in pre-mRNAs is very important in this splicing process and requires some protein auxiliary factors such as U2 small nuclear ribonucleoprotein auxiliary factor small subunit (U2AF35, encoded by U2AF1). By its RNA binding domains, U2AF35 interacts with U2AF65 to bind 3′ splice site of pre-mRNA and initiates splicing. Another protein, which is named as U2AF1-like3 (U2AF1L3), shows high similarity with U2AF35 and may have related function in pre-mRNA splicing. Here, we report a splice variant of U2AF1L3, which is 767 bp in length and has an open reading frame (ORF) coding a predicted 181 amino acids protein. Reverse transcription-PCR (RT-PCR) shows that this isoform has different expression pattern with U2AF1L3 and is highly expressed in heart, brain and lung.
  07/2009; 17(4):282-286.
• Source

  Available from: bioscience.org

  Article: Identification of genes differentially regulated by transcription factor, AP-2delta.

  Liyun Sun, Shengdong Huang, Qihan Wu, Shaohua Gu, Xuping Fu, Ke Yu, Fanglin Lu, Chaoneng Ji, Congjing Feng, Ruping Sun, Yi Xie, Yumin Mao
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  ABSTRACT: In an effort to better understand the transcriptional program regulated by transcription factor, AP-2delta, we used cDNA microarray to evaluate the relative expression of human genes in AD293 cells by exogenous expression of AP-2delta. Microarray showed 29 genes that were up-regulated and 39 genes with a down-regulated expression pattern. Among the identified genes were those encoding transcription factors, signal transduction molecules, kinases, as well as genes regulating cell growth, differentiation, and cell adhesion, a set of genes suggested to be regulated by AP-2. The results of microarray was validated for some regulated genes by real-time PCR analysis. These observations shed novel insight into the mechanism of AP-2delta action and provide a range of target genes for further investigation.
  Frontiers in Bioscience 02/2007; 12:1699-706. · 3.52 Impact Factor
• Article: Cloning and characterization of a novel splice variant of human U2AF1L3 gene.

  Fei Chen, Chaoneng Ji, Tonghai Dou, Nan Zheng, Rui Qiu, Jing Peng, Weiqun Fang, Congjing Feng, Yi Xie, Yumin Mao
  [show abstract] [hide abstract]
  ABSTRACT: Pre-mRNA splicing allows individual genes to produce multiple protein isoforms with diverse functions. Recognition of functional splice sites in pre-mRNAs is very important in this splicing process and requires some protein auxiliary factors such as U2 small nuclear ribonucleoprotein auxiliary factor small subunit (U2AF35, encoded by U2AF1). By its RNA binding domains, U2AF35 interacts with U2AF65 to bind 3' splice site of pre-mRNA and initiates splicing. Another protein, which is named as U2AF1-like3 (U2AF1L3), shows high similarity with U2AF35 and may have related function in pre-mRNA splicing. Here, we report a splice variant of U2AF1L3, which is 767 bp in length and has an open reading frame (ORF) coding a predicted 181 amino acids protein. Reverse transcription-PCR (RT-PCR) shows that this isoform has different expression pattern with U2AF1L3 and is highly expressed in heart, brain and lung.
  DNA Sequence 09/2006; 17(4):282-6. · 0.75 Impact Factor
• Article: Characterization of a novel human protein phosphatase 2C family member, PP2Ckappa.

  Jianfeng Dai, Jiyuan Zhang, Yaqiong Sun, Qihan Wu, Liyun Sun, Chaoneng Ji, Shaohua Gu, Congjing Feng, Yi Xie, Yumin Mao
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  ABSTRACT: A novel member of human protein phosphatase 2C gene named PP2Ckappa was isolated from a human fetal brain cDNA library. The 2.0 kb cDNA encodes a 372 amino acid polypeptide with an intact protein phosphatase 2C (PP2C) catalytic domain. Reverse transcription-PCR (RT-PCR) revealed that the PP2Ckappa was widely expressed in normal human tissues. Transient transfection suggested that PP2Ckappa was localized in the nucleus in AD293 cells. Recombinant Trx-His-PP2Ckappa showed phosphatase activity toward p-nitrophenyl phosphate (pNPP), as well as oligopeptides containing phospho-threonine residues. Furthermore, the overexpression of PP2Ckappa distinctly activated the heat shock transcription factor pathway in eukaryotic cells.
  International Journal of Molecular Medicine 07/2006; 17(6):1117-23. · 1.98 Impact Factor
• Article: Dual specificity phosphotase 18, interacting with SAPK, dephosphorylates SAPK and inhibits SAPK/JNK signal pathway in vivo.

  Qihan Wu, Shengdong Huang, Yaqiong Sun, Shaohua Gu, Fanglin Lu, Jianfeng Dai, Gang Yin, Liyun Sun, Dan Zheng, Chao Dou, Congjing Feng, Chaoneng Ji, Yi Xie, Yumin Mao
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  ABSTRACT: The SAPK/JNKs play important roles in numerous cellular processes, and for this reason they have become putative drug targets. Most dual-specificity protein phosphatases (DSPs) play important roles in the regulation of mitogenic signal transduction and cell cycle control in response to extracellular stimuli. Dual-specificity phosphatase 18 (DUSP18), a newly recognized SAPK/JNK phosphatase, is widely expressed. This expression is modulated in response to extracellular stimuli. By phosphorylation assay, pull down and coimmunoprecipitation experiments, it is shown here that DUSP18 interacts with SAPK/JNK and dephosphorylates it both in vitro and in vivo. DUSP18 does not dephosphorylate p38 or p44ERK1. Furthermore, DUSP18 inhibits SAPK/JNK pathway in vivo. Based on these findings, DUSP18 appears to serve an important role by regulation of SAPK/JNK pathway.
  Frontiers in Bioscience 02/2006; 11:2714-24. · 3.52 Impact Factor
• Article: Molecular cloning and characterization of a novel human putative transmembrane protein homologous to mouse sideroflexin associated with sideroblastic anemia.

  Huarui Zheng, Chaoneng Ji, Xianqiong Zou, Maoqing Wu, Zhe Jin, Gang Yin, Jixi Li, Congjing Feng, Haipeng Cheng, Shaohua Gu, Yi Xie, Yumin Mao
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  ABSTRACT: Sideroflexin1 (Sfxn1), the prototype of a novel family of evolutionarily conserved proteins present in eukaryotes, has been found mutated in mice with siderocytic anemia. It is speculated that this protein facilitates the transport of a component required for iron utilization into mitochondrial. During the large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA encoding a novel sideroflexin protein (SFXN4), which showed 59% identity and 71% similarity to mouse sideroflexin4. According to the search of the human genome database, SFXN4 gene is mapped to chromosome 10q25-26 and spans more than 24.7kb of the genomic DNA. It is 1428 base pair in length and the putative protein contains 305 amino acids with a conserved predicted five-transmembrane-domains structure. RT-PCR result shows that the SFXN4 gene is expressed in many tissues.
  DNA Sequence 11/2003; 14(5):369-73. · 0.75 Impact Factor