[show abstract][hide abstract] ABSTRACT: The genomic region encompassing the L protein gene and a small open reading frame (ORF 6) of Rice yellow stunt virus (RYSV) has been sequenced, thus completing the nucleotide sequence of the RYSV genome. The genome organization of RYSV is unique in the rhabdoviruses because it contains two additional genes when compared to the basic gene order of the family Rhabdoviridae: Phylogenetic analysis revealed that the amino acid sequence of the RYSV L protein is most closely related to that of the L protein of Sonchus yellows net virus, another nucleorhabdovirus. However, the RYSV L protein has a unique acidic N-terminal domain distinct from that of other rhabdoviruses. Moreover, the polypeptide encoded by the ORF 6 was detected by immunoblot analysis in purified RYSV virions. Thus RYSV provides the first example in the family Rhabdoviridae that a small ORF between the G and L genes encodes a virion protein.
Journal of General Virology 09/2003; 84(Pt 8):2259-64. · 3.13 Impact Factor
[show abstract][hide abstract] ABSTRACT: The 3′ leader and the 5′ trailer of the rice yellow stunt rhabdovirus (RYSV) genomic RNA have been cloned and sequenced. Sequence
data indicate that the RYSV leader region is composed of 203 nucleotides (nt) and the trailer region 191 nt. The terminal
9 nt of the two regions are complementary and capable of forming a putative panhandle structure common to rhabdovirus genomes.
In comparison with the leader or trailer sequences of other rhabdoviruses reported so far, both the leader and trailer of
RYSV are the longest and there is no obvious sequence homology between the counterparts except for a few terminal nt and the
UGUU motif in the leader sequences. Polyadenylated plus-strand leader RNA has been detected in RYSV-infected rice plants by
3′ RACE. This is the second example in rhabdoviruses following the report for sonchus yellow net virus (SYNV) for existence
of a polyadenylated leader RNA. No polyadenylated plus-strand transcripts of the RYSV trailer have been found using the similar
Science in China Series C Life Sciences 01/1999; 42(1):50-56. · 1.61 Impact Factor