Zhiming Yu

Academy of Chinese Culture of Health Sciences, Florida, United States

Are you Zhiming Yu?

Claim your profile

Publications (39)47.72 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: We studied the possible role that marine microalgae may play during the outbreaks of WSS (white spot syndrome). In order to elucidate the possibility of marine microalgae carrying WSSV (white spot syndrome virus), six marine microalgae (Isochrysis galbana, Skeletonema costatum, Chlorella sp., Heterosigma akashiwo, Scrippsiella trochoidea, Dunaliella salina) were co-cultured with adult Marsupenaeus japonicus infected with WSSV and were assayed daily by nested-PCR to study whether they could carry WSSV. Further experiments were conducted to investigate whether the virus carried by microalgae could re-infect juvenile M. japonicus. Results showed that all of the experimental microalgae, except H. akashiwo could carry WSSV, and among them, Chlorella sp. and S. trochoidea had the strongest WSSV-carrying ability. Unlike other invertebrate carriers of WSSV, the WSSV detections in microalgae, which were positive after 1 and 3 days, were negative after 10days of incubation. WSSV detection results in juvenile M. japonicus showed that the juvenile shrimp were re-infected by co-cultured Chlorella sp., although the juvenile M. japonicus carried so small an amount of WSSV that it could only be detected by nested-PCR. The results of this experiment suggest that microalgae might be one possible horizontal transmission pathway for WSSV. Further research, however, is required to better understand the factors behind the different carrying abilities and virus-carrying mechanisms of different microalgae.
    Journal of Invertebrate Pathology 07/2007; 95(2):87-92. · 2.67 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The effects of fresh thalli and culture medium filtrates from two species of marine macroalgae, Ulva pertusa Kjellm (Chlorophyta) and Gracilaria lemaneiformis (Bory) Dawson (Rhodophyta), on growth of marine microalgae were investigated in co-culture under controlled laboratory conditions. A selection of microalgal species were used, all being identified as bloom-forming dinoflagellates: Prorocentrum donghaiense Lu sp., Alexandrium tamarense (Lebour) Balech, Amphidinium carterae Hulburt and Scrippsiella trochoide (Stein) Loeblich III. Results showed that the fresh thalli of either U. pertusa or G. lemaneiformis significantly inhibited the microalgal growth, or caused mortality at the end of the experiment. However, the overall effects of the macroalgal culture filtrates on the growth of the dinoflagellates were species-specific (inhibitory, stimulatory or none) for different microalgal species. Results indicated an allelopathic effect of macroalga on the co-cultured dinoflagellate. We then took P. donghaiense as an example to further assess this hypothesis. The present study was carried out under controlled conditions, thereby excluded the fluctuation in light and temperature. Nutrient assays showed that nitrate and phosphate were almost exhausted in G. lemaneiformis co-culture, but remained at enough high levels in U. pertusa co-culture, which were well above the nutrient limitation for the microalgal growth, when all cells of P. donghaiense were killed in the co-culture. Daily f/2 medium enrichment greatly alleviated the growth inhibition on P. donghaiense in G. lemaneiformis co-culture, but could not eliminate it. Other environmental factors, such as carbonate limitation, bacterial presence and the change of pH were also not necessary for the results. We thus concluded that allelopathy was the most possible reason leading to the negative effect of U. pertusa on P. donghaiense, and the combined roles of allelopathy and nutrient competition were essential for the effect of G. lemaneiformis on P. donghaiense.
    Aquatic Botany. 01/2007;
  • Source
    You Wang, Xuexi Tang, Yongqi Li, Zhiming Yu
    [Show abstract] [Hide abstract]
    ABSTRACT: Healthy sporophytes of two gametophyte mutants of Laminaria japonica with different heat resistances: kelp 901 (901, with comparatively stronger heat-resistance) and Rongcheng No.1 (RC, sensitive to heat stress), were respectively collected during October to December 2002 from Yantai and Rongcheng Sea Farm in the Shandong Peninsula of China. The contents of some biochemical materials and antioxidant capacity were analyzed under controlled laboratory conditions to identify if there is any relation between the overall antioxidant capacity and the heat-resistance in L. japonica and to understand possible mechanism of heat-resistance. Results show that: (1) the overall antioxidant capacity in healthy sporophyte of 901, such as vitamin E, polyphenol, and ascorbic acid contents and the enzymatic activity of SOD, POD, CAT, Gpx, PPO, and PAL, were not always higher than that of RC under controlled laboratory conditions, and no significance (P>0.05) was shown in total antioxidant capacity (T-AOC) in 901 and RC. Result suggested that the difference in antioxidant capacity was not a decisive factor for different heat-resistances in L. japonica; (2) the simultaneous assay on isozymes was carried out using vertical polyacrylamide gel electrophoresis (PAGE). Considerable differences in peroxide (PRX), malate dehydrogenase (MDH), malic enzyme (ME), polyphenol oxidase (PPO) and glutamate dehydrogenase (GDH) were obtained in 901 and RC from either the band number, relative mobility (R f ), or staining intensity, and ME could be used as an indicator to distinguish healthy sporophyte of 901 and RC under controlled laboratory conditions.
    Chinese Journal of Oceanology and Limnology 12/2006; 25(1):67-72. · 0.58 Impact Factor
  • Yu Li, Zhiming Yu, Xiuxian Song, Qinglin Mu
    [Show abstract] [Hide abstract]
    ABSTRACT: Suspended particulate matter (SPM), sediments and clams were collected at three sites in Jiaozhou Bay to assess the magnitude of trace metal pollution in the area. Metal concentrations in SPM (Cu: 40.11-203; Zn: 118-447; Pb: 50.1-132; Cd: 0.55-4.39; Cr: 147.6-288; Mn: 762-1670 microg/g), sediments (Cu: 17.64-34.26; Zn: 80.79-110; Pb: 24.57-49.59; Cd: 0.099-0.324; Cr: 41.6-88.1; Mn: 343-520 microg/g) and bivalves (Cu: 6.41-19.76; Zn: 35.5-85.5; Pb: 0.31-1.01; Cd: 0.51-0.67; Mn: 27.45-67.6 microg/g) are comparable to those reported for other moderately polluted world environments. SPM showed a less clear pattern. Metal concentrations in sediments displayed a clear geographical trend with values increasing with proximity to major urban centers. The clams (on dry weight) showed a complex pattern due to the variability introduced by age-related factors. Cd showed an apparent reverse industrial trend with higher concentrations in clams collected at distant stations. Zn, Pb and Mn showed no clear geographical pattern, whereas Cu increased in the clams collected in the most industrialized area. In addition, the bioaccumulation factors (BAF) were calculated. The result indicated that the studied Ruditapes philippinarum in Jiaozhou Bay possessed different bioaccumulation capacities for Cd, Zn, Cu, Pb and Mn, and Cd, Zn had a relatively high assimilation of those metals from sediment particles. A significant relationship with clam age was observed for Zn (positive) and Cu (negative) suggesting different physiological requirements for both metals with age. Trace metal concentrations measured in the tissue of the investigated clam were in the range considered safe by the WHO for human use.
    Environmental Monitoring and Assessment 11/2006; 121(1-3):491-501. · 1.59 Impact Factor
  • Wu Jifa, Zhiming Yu, Song Xiuxian, Wang You
    [Show abstract] [Hide abstract]
    ABSTRACT: Fish Lateolabrax japonicus were exposed to 0.1 and 1mg/L of anion surfactant sodium dodecylbenzene sulfonate (SDBS) and to 2 and 20 microg/L of benzo[a]pyrene (B[a]P) for 6, 12, and 18 days, with control and solvent control groups. Liver antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and glutathione S-transferase (GST), were determined; brain acetylcholinesterase (AChE) and liver inducible nitric oxide synthase (iNOS) activities were also measured. The results indicated that (1) L. japonicus avoided oxidative damage through antioxidant systems; (2) SOD, GPx, and GSH were induced, and GST was inhibited and then induced by B[a]P exposure; and (3) CAT, GPx, and AChE were induced while iNOS was inhibited, and GST was induced and then inhibited by SDBS stress in experimental period.
    Ecotoxicology and Environmental Safety 11/2006; 65(2):230-6. · 2.20 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In order to observe the effect of salinity on disease resistance and white spot syndrome virus (WSSV) proliferation in Fenneropenaeus chinensis, shrimps with latent WSSV were subjected to two acute salinity changes from the original salinity of 22 ppt to 18 and 14 ppt in an hour, respectively. The total haemocyte count (THC) of the challenged group showed no evident change under salinity adjustments, but the phenoloxidase (PO) index declined significantly (P < 0.05) corresponding to continuing acute salinity changes from the 24th to the 72nd hour. According to the WSSV load detected by quantitative real-time PCR method, it was found that WSSV carried by the challenged group and control group were significantly different (P < 0.05); acute salinity change from 22 to 14 ppt led to the WSSV carried in the challenged group being significantly higher (P < 0.05) than that of those surviving in 22 ppt, but salinity change from 22 to 18 ppt had no such effect. At the end of the 72-h experiment, the challenged group subjected to salinity change from 22 to 14 ppt had nearly 3 times the WSSV load as the control group with no salinity change. Therefore, salinity changes over a particular range could result in a decrease of immunocompetence and obvious WSSV proliferation in the shrimps, leading to white spot syndrome developing from a latent infection to an acute outbreak.
    Aquaculture. 01/2006;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Interactions between Prorocentrum donghaiense and Alexandrium tamarens, two bloom-forming dinoflagellates, were investigated using bi-algal cultures. All P. donghaiense died, but A. tamarense was hardly affected by the end of the experiment when the initial cell density was set at 1.0×104 cells mL−1 for P. donghaiense and 0.28×104 cells mL−1 for A. tamarense. However, significant growth suppression occurred in either species when the initial cell density of P. donghaiense increased to 1.0×105 cells mL−1 in the bi-algal culture, but no out-competement was observed. The simultaneous assay on the culture filtrates showed that P. donghaiense filtrate prepared at a lower initial density (1.0×104 cells mL−1) stimulated growth of the co-cultured A. tamarense (0.28×104 cells mL−1), but filtrate at a higher initial density (1.0×105 cells mL−1) depressed its growth. The filtrate of A. tamarense at a density of 0.28×104 cells mL−1 killed all P. donghaiense at a lower density (1.0×104 cells mL−1), but only exhibited an inhibitory effect on it at a higher density (1.0×105 cells mL−1). It is likely that these two species of microalgae interfere with each other mainly by releasing allelochemical substance(s) into the culture medium, and a direct cell-to-cell contact was not necessary for their mutual interaction. The allelopathic test further proved that A. tamarense could affect the growth of co-cultured P. donghaiense by producing allelochemical(s); moreover, A. tamarense culture filtrate at the stationary growth phase (SP) had a strongly inhibitory effect on P. donghaiense compared to that at the exponential phase (EP). Results also demonstrated a dose-dependent relationship between the microalgal initial cell density and the degree of the allelopathic effect. The growth of P. donghaiense and A. tamarense in the bi-algal cultures was simulated using a mathematical model to quantify the interaction. The estimated parameters from the model showed that the inhibition exerted by A. tamarense on P. donghaiense was about 17 and 8 times stronger than the inhibition P. donghaiense exerted on A. tamarense, when the initial cell density was set at 1.0×104 and 1.0×105 cells mL−1 for P. donghaiense, respectively, and 0.28×104 cells mL−1 for A. tamarense in the bi-algal cultures. A. tamarense seems to have a survival strategy that is superior to that of P. donghaiense in bi-algal cultures under controlled laboratory conditions.
    Journal of Sea Research - J SEA RES. 01/2006; 56(1):17-26.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Eutrophication has become increasingly serious and noxious algal blooms have been of more frequent occurrence in the Yangtze River Estuary and in the adjacent East China Sea. In 2003 and 2004, four cruises were undertaken in three zones in the estuary and in the adjacent sea to investigate nitrate (NO3–N), ammonium (NH4–N), nitrite (NO2–N), soluble reactive phosphorus (SRP), dissolved reactive silica (DRSi), dissolved oxygen (DO), phytoplankton chlorophyll a (Chl a) and suspended particulate matter (SPM). The highest concentrations of DIN (NO3–N+NH4–N+NO2–N), SRP and DRSi were 131.6, 1.2 and 155.6μM, respectively. The maximum Chl a concentration was 19.5mgm−3 in spring. An analysis of historical and recent data revealed that in the last 40years, nitrate and SRP concentrations increased from 11 to 97μM andfrom 0.4 to 0.95μM, respectively. From 1963 to 2004, N:P ratios also increased from 30–40 up to 150. In parallel with the N and P enrichment, a significant increase of Chl a was detected, Chl a maximum being 20mgm−3, nearly four times higher than in the 1980s. In 2004, the mean DO concentration in bottom waters was 4.35mgl−1, much lower than in the 1980s. In comparison with other estuaries, the Yangtze River Estuary was characterized by high DIN and DRSi concentrations, with low SRP concentrations. Despite the higher nutrient concentrations, Chl a concentrations were lower in the inner estuary (Zones 1 and 2) than in the adjacent sea (Zone 3). Based on nutrient availability, SPM and hydrodynamics, we assumed that in Zones 1 and 2 phytoplankton growth was suppressed by high turbidity, large tidal amplitude and short residence time. Furthermore, in Zone 3 water stratification was also an important factor that resulted in a greater phytoplankton biomass and lower DO concentrations. Due to hydrodynamics and turbidity, the open sea was unexpectedly more sensitive to nutrient enrichment and related eutrophication processes.
    Hydrobiologia 01/2006; 563(1):313-328. · 1.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains ofProrocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo I, Hae III, and RSA I) and then resolved in agarose gels. Results show that different species had different RFLP patterns, except forP. arcuatum (ME 131), which had the same pattern toP. micans (ME160 and 04). The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electrophoresis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthicProrocentrum species were different from each other, and also from other cultured ones examined in this study.P arcuatum (ME132) could not be differentiated fromP. micans (ME160 and 04), it was probably mis-identified, since they are quite different morphologically.P. redfieldii (ME138) could also not be distinguished formP. triestinium (ME132), it should be regarded as a synonym ofP. triestinium. Unexpectedly, a restriction site was found inP. micans, compared with previous sequence data.
    Chinese Journal of Oceanology and Limnology 01/2005; 23(3):317-322. · 0.58 Impact Factor
  • Xihua Cao, Zhiming Yu
    [Show abstract] [Hide abstract]
    ABSTRACT: Periodic and widespread algal blooms have caused a variety of problems for aquatic life and human activity throughout the world. Currently, the only remedial practice employed for removing algal blooms is to spread clay on the surface of the water. But, the algal removal efficiency by the crude minerals are not really ideal, and how to improve the capability of clays to remove algae is now the technological focus, which also is fatal to the practical value of clays. In this study, hexadecyltrimethyleamine bromide (HDTMAB), one kind of cationic organo-surfactants, was chosen to improve kaolin by surface sorption and cationic exchange, and was tested to remove Prorocentrum donghaiense, a red tide organism in Donghai Sea. The results indicated that organo-clay had an excellent ability to extinguish red tide organisms, even under the application of 0.01 g.L-1, and could subside more than 95% red tide organisms in 24 h. The efficient algae removal by organo-clay might be from the reversal of the surface electric charge on clay particles, the "net capture" by the long lipoid chains of HDTMAB, and the local high density of HDTMAB on particles surface, which could effectively kill algal cells.
    Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 08/2003; 14(7):1169-72.
  • Zhiming Yu, Caiwen Li, Yueqiang Guan
    Ophelia. 08/2003; 57(2):99-106.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effects of strain S10 isolated from sediments of Xiamen Western Sea Area on the growth and paralytic shellfish poison (PSP) production of Alexandrium tamarense at different pH and salinities were studied. The results showed that the alga grew well at pH 6-8 and at salinity of 20-34. The toxicity of A. tamarense varied markedly at different pH and salinities: it decreased with increasing pH, while increased with salinity and reached its peak value at the salinity of 30, and then declined. The strain S10 inhibited the growth and the PSP production of A. tamarense at different pH and salinities. It had the best inhibitory function on the growth of A. tamarense at pH 7 and salinity of 34. The best inhibitory function on the PSP production of A. tamarense was at pH 7, but this inhibitory function was not related to salinity.
    Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 08/2003; 14(7):1161-4.
  • Yueqiang Guan, Zhiming Yu, Caiwen Li
    Journal of Invertebrate Pathology 08/2003; 83(3):257-60. · 2.67 Impact Factor
  • Xiuxian Song, Zhiming Yu, Yonghui Gao
    [Show abstract] [Hide abstract]
    ABSTRACT: An effective clay-complex system was composed on the base of clay by added other two components A and B. Different red tide species, such as Scrippsiella trochoidea, Amphidinium carterae and Heterosigma akashiwo, were coagulated by this clay-complex system, and the optimum conditions for removal red tide organisms were obtained by means of orthogonal test. The results showed that clay was the most important factors in this complex system to coagulate these three organisms. The removal efficiency on three species was in order of Scrippsiella trochoidea > Amphidinium carterae > Heterosigma akashiwo. At the same time, a preliminary study of mortality rate on Penaeus japonicus (length between 1.0 to 1.5 cm) was conducted with this system, and the result of toxic test revealed that the mortality of aquaculture shrimp in control group (nothing added) reached to 80% after 96 h, however, while that of other three groups which added clay and other two components A and B, was below 40%. Thus, it implied that the addition of clay would be harmless to Penaeus japonicas. Furthermore, suitable concentration of components A and B was not harmful for the aquaculture shrimp, but increased the removal efficiency of red tide organisms. The preliminary results showed that this clay-complex system would be available in the removal of red tide organisms in filed.
    Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 08/2003; 14(7):1165-8.
  • Zhiming Yu, Xiuxian Song
    [Show abstract] [Hide abstract]
    ABSTRACT: Matrix-bound phosphine (PH3), a new form of phosphorus, was found in sediment of Jiaozhou Bay in December 2001. Concentration and distribution of PH3 in different layers of sediment with different stations were analyzed. The results show that PH3 concentrations are various with different layers and different stations. PH3 concentrations in the bottom layer of sediment (20–30 cm) are usually higher than those in the surface layer (0–4 cm). The highest PH3 concentration in our investigation reaches 685 ng/kg (dry), which is much higher than those in terrestrial paddy soil, marsh and landfill that have been reported up to now. The correlation analysis indicates that there is no apparent correlation between the concentrations of PH3 and inorganic phosphorus in sediment. However, the correlation between the concentrations of phosphine and organic phosphorus in the bottom layer of sediment is remarkable (R2=0.83). It is considered that PH3 in sediment of Jiaozhou Bay is mainly produced from the decomposition of organic phosphorus in the anaerobic condition, and so PH3 concentrations are related to organic phosphorus concentration and anaerobic environment in sediment. The discovery of PH3 in sediment will give people some new ideas on the mechanisms of phosphorus supplement and biogeochemical cycle in Jiaozhou Bay. KeywordsJiaozhou Bay-sediment-phosphine-phosphorus cycle
    Chinese Science Bulletin 01/2003; 48(1):31-35. · 1.32 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This work was supported by the National Natural Science Foundation of China under contract No.30070175and No.40206015.
    01/2003;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Surface modification of montmorillonite by means of Mg2+ insertion reaction has been studied and a positively charged montrnorillonite has been prepared. The effects of preparation temperature and Mg+ concentration on the positive charge property of the clay and on the clay coagulatingHeterosigma akashiwo have been studied. The results showed that the modified clay enhanced the coagulation and the used amount decreased to 1/5–1/10 of the original. The removal rates ofHeterosigma akashiwo were correlated positively with positive charge on the clay in accordance with theoretical model. Keywordsmontmorillonite modification-coagulation-red tide organisms
    Chinese Science Bulletin 01/1999; 44(7):617-620. · 1.32 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 µmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = −3.305x +98.95, R 2 = 0.988, where x represents nitrate concentrations (<40 µmol/L) and y represents the Nrt2 transcript levels.
    Acta Oceanologica Sinica -English Edition- 32(6). · 0.58 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Both nitrate (NO3−) and soluble reactive phosphate (PO43−) concentration in the freshwater end-member at the mouth of the Changjiang River have increased dramatically since the 1960s. Within the same period in the sea area, with surface salinity>30, NO3− concentration has shown an obvious increase, PO43− has not changed greatly and dissolved reactive silica (SiO32−) has deceased dramatically. An examination of the elemental ratio of NO3− to PO43− at the mouth of the Changjiang River did not show a systematic trend from the 1960s to 2000s largely because both nutrients increased simultaneously. In comparison, the elemental ratio of dissolved inorganic nitrogen (DIN) to PO43− in surface seawater, with salinity>22, has shown a clearly increasing trend. Furthermore, an overall historical change of the SiO32−:PO43− ratio has undergone a reverse trend in this area. Based on the changes of SiO32−:PO43− and DIN:PO43− ratios, we can conclude that an overall historical change of SiO32−:DIN ratio has decreased in this area from the 1950–1960s to 2000s. The argument that phytoplankton productivity in the Changjiang estuary has been enhanced by increasing nutrient input from the riverine transport was supported by these results. A comparative study analyzing the shift of phytoplankton composition from the mid-1980s to 2000s was also made. The results indicated that the average yearly percentage of diatom species in the Changjiang estuary has decreased from 84.6% during 1985–1986 to 69.8% during 2004–2005. Furthermore, the average yearly percentage of diatom abundance in the Changjiang estuary decreased from 99.5% during to 75.5% over the same time period, while the abundance of dinoflagellates has increased dramatically, from 0.7% to 25.4%. Keywordeutrophication-nutrients-phytoplankton community-Changjiang estuary
    Chinese Journal of Oceanology and Limnology 28(4):887-898. · 0.58 Impact Factor

Publication Stats

298 Citations
47.72 Total Impact Points

Institutions

  • 2013–2014
    • Academy of Chinese Culture of Health Sciences
      Florida, United States
  • 2005–2011
    • Northeast Institute of Geography and Agroecology
      • • Key Laboratory of Marine Ecology and Environmental Sciences
      • • Institute of Oceanology
      Beijing, Beijing Shi, China
  • 2008
    • Chinese Academy of Sciences
      • Institute of Oceanology
      Peping, Beijing, China
  • 2003
    • Xiamen University
      • School of Life Sciences
      Xiamen, Fujian, China