Randy D Rutkowske

Publications (9)15.9 Total impact

• Article: Frequency-swept HMQC sequences for high-throughput NMR analysis.

  Timothy D Spitzer, Randy D Rutkowske, George F Dorsey
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  ABSTRACT: We describe here new versions of the DEPT phase-encoded HMQC experiment that offer robust performance and improved sensitivity. The new sequences rely on frequency-swept proton and carbon pulses to minimize signal losses from miscalibrated pulses while providing 'J compensation' to optimize the signal strength over a range of heteronuclear coupling constants. By including both proton and carbon-swept pulses, the new sequences also offer an additional signal gain of roughly 10% over well-calibrated hard-pulse experiments. The new sequences also demonstrate that one can construct a sequence that incorporates both 90 degrees and 180 degrees frequency-swept pulses. Although individual pulses in the sequence cause severe phase roll, the phase roll can be eliminated by the proper choice of pulse lengths and sweep directions.
  Magnetic Resonance in Chemistry 07/2008; 46(6):558-63. · 1.44 Impact Factor
• Article: Frequency-swept HSQC sequences for high-throughput NMR analysis.

  Timothy D Spitzer, Randy D Rutkowske, George F Dorsey
  [show abstract] [hide abstract]
  ABSTRACT: This article describes new versions of the DEPT phase-edited heteronuclear single quantum correlation (HSQC) pulse sequence with sensitivity enhancement. The sequences incorporate frequency-swept carbon and proton pulses. The new experiments are inherently robust, well-suited for a high-throughput setting in which sample-to-sample variations may be ignored. The observed signal has the obvious benefit of sensitivity enhancement resulting from the preservation of two magnetization transfer pathways. The two pathways are maintained even in the version of the sequence in which all pulses are frequency-swept. There is an additional signal gain of roughly 10% that derives from the use of both proton and carbon frequency-swept pulses. Furthermore, the sequences use J compensation to provide optimal signal over a range of heteronuclear coupling constants. We demonstrate that the new sequences offer good sensitivity and perform well even when the NMR probe is deliberately mistuned.
  Magnetic Resonance in Chemistry 07/2008; 46(6):564-70. · 1.44 Impact Factor
• Article: Automated structure verification based on a combination of 1D (1)H NMR and 2D (1)H - (13)C HSQC spectra.

  Sergey S Golotvin, Eugene Vodopianov, Rostislav Pol, Brent A Lefebvre, Antony J Williams, Randy D Rutkowske, Timothy D Spitzer
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  ABSTRACT: A method for structure validation based on the simultaneous analysis of a 1D (1)H NMR and 2D (1)H - (13)C single-bond correlation spectrum such as HSQC or HMQC is presented here. When compared with the validation of a structure by a 1D (1)H NMR spectrum alone, the advantage of including a 2D HSQC spectrum in structure validation is that it adds not only the information of (13)C shifts, but also which proton shifts they are directly coupled to, and an indication of which methylene protons are diastereotopic. The lack of corresponding peaks in the 2D spectrum that appear in the 1D (1)H spectrum, also gives a clear picture of which protons are attached to heteroatoms. For all these benefits, combined NMR verification was expected and found by all metrics to be superior to validation by 1D (1)H NMR alone. Using multiple real-life data sets of chemical structures and the corresponding 1D and 2D data, it was possible to unambiguously identify at least 90% of the correct structures. As part of this test, challenging incorrect structures, mostly regioisomers, were also matched with each spectrum set. For these incorrect structures, the false positive rate was observed as low as 6%.
  Magnetic Resonance in Chemistry 11/2007; 45(10):803-13. · 1.44 Impact Factor
• Article: An investigation of the absolute configuration of the potent histamine H3 receptor antagonist GT-2331 using vibrational circular dichroism.

  Douglas J Minick, Royston C B Copley, Jerzy R Szewczyk, Randy D Rutkowske, Luke A Miller
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  ABSTRACT: GT-2331 [(+)-1] is one of the most potent members of a class of chiral drug substances used to regulate the synthesis and release of histamine by the histamine H3 receptor, and as such, is an important biomarker for pharmaceutical companies conducting research in this field. In addition to overall structural features, the bioactivity of this molecule has also been found to be highly dependent on absolute stereochemistry, making the reliable assignment of this property a necessity. X-ray diffraction studies have provided conflicting data, leaving its three-dimensional structure uncertain. In view of this, its absolute configuration was investigated by vibrational circular dichroism. Results from this study provided independent assignment of this important molecule as the (1S,2S)-enantiomer.
  Chirality 10/2007; 19(9):731-40. · 2.35 Impact Factor
• Article: Automated structure verification based on a combination of 1D 1H NMR and 2D 1H13C HSQC spectra

  Sergey S. Golotvin, Eugene Vodopianov, Rostislav Pol, Brent A. Lefebvre, Antony J. Williams, Randy D. Rutkowske, Timothy D. Spitzer
  [show abstract] [hide abstract]
  ABSTRACT: A method for structure validation based on the simultaneous analysis of a 1D 1H NMR and 2D 1H13C single-bond correlation spectrum such as HSQC or HMQC is presented here. When compared with the validation of a structure by a 1D 1H NMR spectrum alone, the advantage of including a 2D HSQC spectrum in structure validation is that it adds not only the information of 13C shifts, but also which proton shifts they are directly coupled to, and an indication of which methylene protons are diastereotopic. The lack of corresponding peaks in the 2D spectrum that appear in the 1D 1H spectrum, also gives a clear picture of which protons are attached to heteroatoms. For all these benefits, combined NMR verification was expected and found by all metrics to be superior to validation by 1D 1H NMR alone. Using multiple real-life data sets of chemical structures and the corresponding 1D and 2D data, it was possible to unambiguously identify at least 90% of the correct structures. As part of this test, challenging incorrect structures, mostly regioisomers, were also matched with each spectrum set. For these incorrect structures, the false positive rate was observed as low as 6%. Copyright © 2007 John Wiley & Sons, Ltd.
  Magnetic Resonance in Chemistry 08/2007; 45(10):803 - 813. · 1.44 Impact Factor
• Article: Optimization of pyrrolidinone based HIV protease inhibitors.

  Ronald G Sherrill, C Webster Andrews, William J Bock, Ronda G Davis-Ward, Eric S Furfine, Richard J Hazen, Randy D Rutkowske, Andrew Spaltenstein, Lois L Wright
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  ABSTRACT: Optimization of P1-substituted pyrrolidinone based HIV protease inhibitors has yielded analogs with very potent antiviral activity.
  Bioorganic & Medicinal Chemistry Letters 02/2005; 15(1):81-4. · 2.55 Impact Factor
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  Available from: Sanjay S. Patel

  Article: Neuromuscular blocking activity and therapeutic potential of mixed-tetrahydroisoquinolinium halofumarates and halosuccinates in rhesus monkeys.

  Eric E Boros, Vicente Samano, John A Ray, James B Thompson, David K Jung, Istvan Kaldor, Cecilia S Koble, Michael T Martin, Virgil L Styles, Robert A Mook, [......], Mir A Hashim, Sanjay S Patel, James C Wisowaty, Gary D Bowers, Caroline L Moseley, John S Walsh, Mindy J Reese, Randy D Rutkowske, Andrea M Sefler, Timothy D Spitzer
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  ABSTRACT: Structure-activity relationships in rhesus monkeys for a novel mixed-onium class of ultra-short-acting nondepolarizing tetrahydroisoquinolinium neuromuscular blockers (NMBs) are described. Bis-onium chlorofumarate 20a with (1R,2S)-benzyltetrahydroisoquinolinium groups was a potent lead compound (ED(95) = 0.079 mg/kg) with an ultra-short duration of NMB effect (7.1 min) and a selectivity index (SI: defined as a ratio of the cardiovascular threshold dose to the ED(95)) similar to that of mivacurium (3). The mean threshold dose for cardiovascular effects with 20a was ca. 20 times its ED(95) value (SI = 20). A novel mixed-onium analogue of 20a was prepared by replacing the benzyltetrahydroisoquinolinium group distal to the fumarate chlorine atom with a (1S,2R)-phenyltetrahydroisoquinolinium moiety. The resulting mixed-onium chlorofumarate 24a displayed good NMB potency (ED(95) = 0.063 mg/kg), ultra-short duration of action (5.6 min) and an improved selectivity index (SI = 57). Several other mixed-onium derivatives containing octanedioate (25a; ED(95) = 0.103 mg/kg), difluorosuccinate (27c; ED(95) = 0.056 mg/kg), and fluorofumarate (28a; ED(95) = 0.137 mg/kg) linkers were also potent, ultra-short-acting NMBs with good to excellent selectivity index values (SI = 37-96). Octanedioate 25a was longer acting at higher doses compared to difluorosuccinate 27c and chlorofumarate 24a. Durations of NMB effect following a 0.4 mg/kg bolus dose (100% block) of 25a, 27c, and 24a were 16.9, 13.0, and 10.0 min, respectively. Recovery time for mixed-onium chlorofumarate 24a following a 1 h continuous infusion at 10-20 microg/kg/min (95-100% block) was ca. 5 min which is similar to that observed following a 0.2 mg/kg bolus dose of this compound and indicates a lack of cummulative effects. Preliminary studies with chlorofumarate 24a in whole human blood revealed that mixed-onium thiazolidine 29 was the major metabolite and that plasma cholinesterases do not play the primary role in duration of NMB effect. The NMB properties of 24a in rhesus monkeys led to its clinical evaluation as a possible alternative to succinylcholine.
  Journal of Medicinal Chemistry 07/2003; 46(12):2502-15. · 5.25 Impact Factor
• Article: Neuromuscular Blocking Activity and Therapeutic Potential of Mixed-Tetrahydroisoquinolinium Halofumarates and Halosuccinates in Rhesus Monkeys

  Eric E. Boros, Vicente Samano, John A. Ray, James B. Thompson, David K. Jung, Istvan Kaldor, Cecilia S. Koble, Michael T. Martin, Virgil L. Styles, Robert A. Mook, Jr, [......], Mir A. Hashim, Sanjay S. Patel, James C. Wisowaty, Gary D. Bowers, Caroline L. Moseley, John S. Walsh, Mindy J. Reese, Randy D. Rutkowske, Andrea M. Sefler, Timothy D. Spitzer
  [show abstract] [hide abstract]
  ABSTRACT: Structure−activity relationships in rhesus monkeys for a novel mixed-onium class of ultra-short-acting nondepolarizing tetrahydroisoquinolinium neuromuscular blockers (NMBs) are described. Bis-onium chlorofumarate 20a with (1R,2S)-benzyltetrahydroisoquinolinium groups was a potent lead compound (ED95 = 0.079 mg/kg) with an ultra-short duration of NMB effect (7.1 min) and a selectivity index (SI:  defined as a ratio of the cardiovascular threshold dose to the ED95) similar to that of mivacurium (3). The mean threshold dose for cardiovascular effects with 20a was ca. 20 times its ED95 value (SI = 20). A novel mixed-onium analogue of 20a was prepared by replacing the benzyltetrahydroisoquinolinium group distal to the fumarate chlorine atom with a (1S,2R)-phenyltetrahydroisoquinolinium moiety. The resulting mixed-onium chlorofumarate 24a displayed good NMB potency (ED95 = 0.063 mg/kg), ultra-short duration of action (5.6 min) and an improved selectivity index (SI = 57). Several other mixed-onium derivatives containing octanedioate (25a; ED95 = 0.103 mg/kg), difluorosuccinate (27c; ED95 = 0.056 mg/kg), and fluorofumarate (28a; ED95 = 0.137 mg/kg) linkers were also potent, ultra-short-acting NMBs with good to excellent selectivity index values (SI = 37−96). Octanedioate 25a was longer acting at higher doses compared to difluorosuccinate 27c and chlorofumarate 24a. Durations of NMB effect following a 0.4 mg/kg bolus dose (100% block) of 25a, 27c, and 24a were 16.9, 13.0, and 10.0 min, respectively. Recovery time for mixed-onium chlorofumarate 24a following a 1 h continuous infusion at 10−20 μg/kg/min (95−100% block) was ca. 5 min which is similar to that observed following a 0.2 mg/kg bolus dose of this compound and indicates a lack of cummulative effects. Preliminary studies with chlorofumarate 24a in whole human blood revealed that mixed-onium thiazolidine 29 was the major metabolite and that plasma cholinesterases do not play the primary role in duration of NMB effect. The NMB properties of 24a in rhesus monkeys led to its clinical evaluation as a possible alternative to succinylcholine.
  05/2003;
• Article: Reactions of Fervenulone. An Unprecedented Ring Contraction of a 7-Azapteridine Ring System

  Mukund M. Mehrotra, Daniel D. Sternbach, Randy D. Rutkowske, Paul L. Feldman
  04/2002;