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Publications (4)3.29 Total impact

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    ABSTRACT: The hypothesis that stem cells may seed cancer has emerged from the cancer stem cells concept. However, the experimental systems necessary to provide more direct evidence to support the hypothesis have been lacking. We have used fetal neural progenitor cells (hNPC) transduced with the telomerase hTERT gene to investigate the neoplastic potential of hNPCs. The hTERT-transduced line, hNPCs-G3 lost normal diploid karyotype, showed loss of contact inhibition, anchorage independence, and formed neuroblastoma-like tumours in all of 10 mice. These data suggest that hNPCs have the potential for neoplastic transformation. These data have implications for providing a novel tool to test the feasibility of new anticancer treatment strategies and raise the possibility of a risk for the use of hNPCs in cell transplantation.
    Neuroreport 09/2004; 15(12):1907-12. DOI:10.1097/00001756-200408260-00015 · 1.64 Impact Factor
  • Yun Bai · Qikuan Hu · Xiaoxia Li · Yajun Wang · Changsheng Lin · Li Shen · Linsong Li
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    ABSTRACT: It is necessary to expand human neural progenitor cells in vitro to obtain large numbers for research purposes and cell transplantation. A potential obstacle to in vitro expansion, however, is that neural progenitor cells have a limited replication life-span and gradually lose their differentiation potential. We report here that ectopic expression of the catalytic subunit of human telomerase (hTERT) gene in neural progenitor cells could induce telomerase activity, stabilize telomeres and extend their replicative life-spans. The telomerase-immortalized cells (hNPC-TERT) maintained the normal diploid karyotype, expressed the markers of human neural progenitor cells and meanwhile held the differentiation potential in vitro for up to 120 population doublings. This study provides a new approach for obtaining unlimited quantities of normal phenotypic and homogeneous human neural progenitor cells in vitro.
    Neuroreport 03/2004; 15(2):245-9. DOI:10.1097/00001756-200402090-00006 · 1.64 Impact Factor
  • Qikuan Hu · Changsheng Lin · Yun Bai · Jie Bai · Qihua He · Li Shen · Lingsong Li
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    ABSTRACT: To investigate whether the dopamine receptor D2 can express or can be induced to express in HNG1210-E, a monoclonal, telomerase-immortalized, human neural progenitor cell line. By means of RT-PCR, immuno-fluorescent staining, and fluo3 Ca2+ imaging to the expression of D2 mRNA and protein as well as the reaction to dopamine were demonstrated. HNG1210-E cells could be induced to express D2 mRNA and its proteins. The induced cells also reacted to dopamine (5 mmol.L-1), which caused rapid rising of cytoplasm Ca2+. HNG1210-E cells can be induced to express D2 mRNA and functional proteins.
    Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 07/2003; 35(3):271-3.
  • Yun Bai · Changsheng Lin · Qikuan Hu · Xiaoxia Li · Aili Lu · Shuling Wang · Lingsong Li · Li Shen
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    ABSTRACT: To investigate the ability of human GFAP positive neural progenitor cell line from the subventricular zone (SVZ) to differentiate into neurons. Real-time RT-PCR, Western blot analysis and immunocytochemistry were used to examine the expression level of the neural stem cell marker and neuronal-specific marker before and after all-trans-retinoic acid (AT-RA) induction in the GFAP positive neural progenitor cell line. Immunocytochemistry was used to examine the expression of the neuronal-specific marker after transplantation the GFAP positive neural progenitor cell line into the animal model. After induction, in the GFAP positive neural progenitor cell line the expression levels of the neuronal-specific marker increased, while the neural stem cell marker decreased both in mRNA and protein levels. After transplantation into animal model, the GFAP positive neural progenitor cell line could differentiate into neurons. The GFAP positive neural progenitor cell line could be induced to differentiate into neurons both in vitro and in vivo.
    Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 07/2003; 35(3):266-70.