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ABSTRACT: We have developed optimum culture conditions for the large-scale propagation of chrysanthemum in balloon-type bioreactors
to achieve vigorous growth and quality. The effects of NH
4
+
/NO
3
−
ratio, air volume, air temperature, photosynthetic photo flux, and an inoculation density on the growth and quality of plantlets
were investigated. The best production conditions were an NH
4
+
:NO
3
−
ratio of 20∶40 mM, air exchange of 0.1 vvm min−1, air temperature 25°C, photosynthetic photo flux (PPF) at 100 μmol m−2 s−1, and an inoculation density of 40 nodes Chrysanthemum grandiflorum. Under each of these conditions, the maximum growth rate reached 279.0, 260,0, 20.0, 23.3, and 94.5 (g-fresh weight per plantlet
d−1), respectively, at 12 wk of culture. These results specify the key environmental factors that can be regulated to improve
the quality and quantity of flowers and increase yield in large-scale bioreactor cultures of chrysanthemum.
In Vitro Cellular & Developmental Biology - Plant 10/2005; 41(6):822-825. · 1.50 Impact Factor
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ABSTRACT: The contents of soluble biophenols such as protocatechuic, gentisic, vanillic, caffeic, syringic, p-coumaric, ferulic, salicylic, and cinnamic acids were screened and quantified from the adventitious roots of Panax ginseng by HPLC-MS. Control adventitious roots which showed the greatest accumulation of ferulic acid (0.09 mg/g DW) were observed
in our experiments. An increase in the total soluble biophenol content of adventitious roots was observed 5 days after treatment
with elicitor 200 µM/L methyl jasmonate (MeJa) i.e., 35 to 40 days of inoculation. Among the biophenols investigated, the
salicylic acid content was higher (0.44 mg/g DW) in MeJa treated adventitious root culture in the bioreactor. MeJa might apparently
increase soluble biophenols production in adventitious roots by enhancing the biosynthesis pathway from phenylalanine to salicylic
acid and other simple biophenols.
Chemistry of Natural Compounds 01/2005; 41(6):669-673. · 1.03 Impact Factor
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ABSTRACT: Phenylalanine and tyrosine play a central role in Gloriosa superba L. calluses for the production of colchicine. The lack of biosynthetic precursors and signal inducing enzyme activity are responsible for the lower production of colchicine in vitro. B 5 medium nutrient grown calluses have a low content of colchicine, indicating that an optimal precursors level is required to increase PAL and TAL activity for colchicine accumulation. These results suggest that precursors are an important regulatory factor in colchicine accumulation in in vitro.
Chemistry of Natural Compounds 08/2004; 40(5):499-502. · 1.03 Impact Factor
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ABSTRACT: An efficient protocol was developed for regeneration of healthy plant derived from six categories of explants from both in vivo and in vitro raised plants, viz. roots, corm buds (dormant and nondormant), young leaves, stems, pedicels, and shoot tips from aerial shoots. MS medium supplemented with various concentrations and combinations of auxin, cytokinin, and organic acids was used. 98% of callus induction occurred in nondormant corm bud explants. The greatest number of multiple shoots (57) was observed in corm-derived calluses. Vigorous root formation occurred in all cases when multiple shoots were derived. Histomorphogenetic studies revealed that not only the origin of shoot and root buds in in vitro systems, but the morphology and structure of leaves resemble those of in vivo plants too.
Russian Journal of Plant Physiology 08/2004; 51(5):713-721. · 0.71 Impact Factor
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ABSTRACT: The induction, maturation and germination of embryoids from leaf tissue of Gloriosa superba L. were developed by exploiting solid and liquid culture. Nodular calli were obtained from SH medium supplemented with 2,4-D and 2iP. In solid culture, the nodular calli when transferred to 2,4-D along with glycerol gave the best response (68.4 %) in embryoid induction after 20 days. After two subcultures at 7-day intervals in a medium with thiamine instead of glycerol, the embryoids matured. When mature embryoids were transferred to BAP and IBA medium, they gave rise to plantlets with single shoots and roots. In liquid culture, the medium supplemented with NAA and L-glutamine with continuous agitation, the embryoidogenic calli produced embryoids (85 %) after 21 days. The mature embryoids began to turn green and produced shoots and elongated "radicles" after 35 days.
Planta Medica 06/2003; 69(5):479-81. · 2.15 Impact Factor
