Chun-Ying Li

Fourth Military Medical University, Xi’an, Liaoning, China

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Publications (11)17.59 Total impact

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    ABSTRACT: Abstract Introduction: Vitiligo is an acquired pigmentary disorder characterized by areas of depigmented skin resulting from the loss of epidermal melanocytes. Recently, several investigations have documented the benefits of excimer phototherapy sources (such as equipment of 308-nm excimer laser and 308-nm excimer lamp) for the treatment of vitiligo. Objective: To compare the effectiveness of 308-nm excimer laser with 308-nm excimer lamp in the treatment of vitiligo patients. Methods: This intervention study was designed as a randomized self control trial. 14 subjects with 48 symmetrical vitiligo lesions were enrolled in this study. One lesion was treated with the 308-nm excimer laser and its counterpart with the 308-nm excimer lamp. Lesions were treated three times a week with the same dose on both sides for a total of 20 sessions. Results: All the patients completed the study and 48 lesions were treated. The two treatments showed similar results in terms of efficacy for a repigmentation. Conclusions: The 308-nm excimer lamp and 308-nm excimer laser showed a similar efficacy in treating vitiligo.
    Journal of Cosmetic and Laser Therapy 05/2013; · 0.86 Impact Factor
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    ABSTRACT: We hypothesize that a novel signaling loop of high-level activation in tumor cells, which is triggered by heparanase overexpression and growth factors and plays a crucial role in tumor progression. It is believed that clarification on the malignant loop will provide us with new ideas about revealing pathogenesis of tumors and define a range of novel and exciting therapeutic approaches.
    Bioscience Hypotheses 01/2008; 1(2):118-120.
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    ABSTRACT: This study was designed to determine the optimal treatment frequency with the 308-nm excimer laser for vitiligo and identify key clinical variable(s) associated with treatment efficacy at the optimal frequency. Optimal clinical parameters for excimer laser treatment of vitiligo have not been fully determined. Data about the influence on treatment frequency of different clinical variables of vitiligo are needed to facilitate effective treatment regimens. A total of 187 patients were treated with the 308-nm excimer laser for 20 sessions at different frequencies (0.5, 1.0, 2.0, and 3.0 per week). The repigmentation rate was graded on a six-point scale and was blindly evaluated by independent physicians. The final percentage of repigmentation for group 0.5 was statistically lower than those for group 1.0, 2.0, and 3.0, and percentages of final levels of repigmentation among these three groups were not statistically different. The clinical variables showed no statistical differences in the final repigmentation effect. Repigmentation occurred fastest with treatment frequencies of 2.0 and 3.0 and there was no statistically significant difference between them. The onset of repigmentation correlated with the area of vitiliginous patches treated, not with the other clinical variables. The 308-nm excimer laser is effective for therapy to treat vitiligo on the face and neck. The ultimate laser-induced repigmentation effect does not correlate with treatment frequency and repigmentation occurs faster with treatment frequencies of 2.0 and 3.0 than that of 1.0. It appears that the onset of repigmentation correlates with the total area of vitiliginous patches and the optimal treatment frequency. Monitored studies on a larger population with long-term follow-up would be needed to confirm and extend our findings.
    Photomedicine and Laser Surgery 11/2007; 25(5):418-27. · 1.63 Impact Factor
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    ABSTRACT: To obtain the specific human scFv against malignant melanoma (MM) using phage antibody library technology. The library was panned with a certain kind of MM cells for 4 rounds. The antigen binding activities of random clones were tested by ELISA in order to select specific antibodies, which were then examined by DNA sequence analysis and immunohistochemistry. In ELISA and immunohistochemical staining, the positive clone selected from the 80 random clones was able to bind one kind of MM cell specifically, while not able to bind other irrelavant cells or antigens, such as human's squamous carcinoma cells, keratinocytes, melanocytes, keratin, trypsinase, transferrin and mouse IgG, etc. The antibody's V(H) genes belonged to human IgG V(H)5 subgroup and the V(L) belonged to human V(kappa) subtype determined by DNA sequence analysis. One specific human phage antibody against MM cells was obtained by phage display technique, which may be useful for further development of tumor-targeted therapies.
    Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 10/2005; 21(5):602-4.
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    ABSTRACT: Antikeratin autoantibodies (AK auto Abs) are very important elements of the human immune system. To improve the outcome of studies on AK auto Abs, it is necessary to generate antikeratin human monoclonal antibodies. The purpose of present study was to isolate antikeratin human monoclonal antibodies by panning a phage antibody library. A semisynthetic phage antibody library with capacity of 4.0x10(8) members was previously constructed. Panning of the library was performed against human epidermal keratin extracted from psoriatic scales. At the last round of the panning, individual colonies were grown in culture for expression of phage antibodies. Their binding activities and specificities to keratin were determined by ELISA, and positive clones were analyzed by DNA fingerprinting. The selected clones were induced with IPTG to express soluble Fab fragments, which were further characterized by ELISA, immunohistochemistry and Western blotting. Finally, DNA sequencing of the variable regions was performed. A human antibody clone which was able to express soluble Fab fragments and recognize Mr 46,000 keratin (K17) was isolated. DNA sequencing demonstrated that the VH and VL of the antibody came from the human VH1 and Vkappa2 families, respectively. We conclude that phage antibody library technology is a powerful way to generate human monoclonal antibodies. The antikeratin antibody we isolated in the present study would be useful in the research on AK auto Abs.
    Archives for Dermatological Research 12/2004; 296(6):270-7. · 2.71 Impact Factor
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    ABSTRACT: To investigate the clinical features and changes in the incidence of skin cancer in two hospitals located in western China. The patients diagnosed pathologically as skin cancer from 1981 to 2000 were retrospectively collected from the two hospitals. Clinical data of patients with skin cancer were collected and analyzed. (1) Of the 1 905 patients with skin cancer, squamous cell carcinoma accounted for 29.4%(560 patients), basal cell carcinoma 28.0% (534), and cutaneous malignant melanoma(CMM) 16.0% (305). (2) There were 591 patients with skin cancer diagnosed between 1980 and 1990, and 1 314 between 1991 and 2000, and accounted for 0.34% and 0.58% of all biopsy cases, respectively. The number of total biopsy patients increased 1.6% every year during the 20 years. The number of biopsy patients with skin cancer and with CMM increased 3.5% and 3.9% every year,respectively. (3) Of the 305 CMM patients, 63.3% located on the acra. These patients were elder, and have a higher rate of trauma and a higher incidence in the male than that in patients with CMM located on the other sites. (4) Of the 305 CMM patients, 64 (21%) had history of trauma at the primary onset sites, and 47 (15.4%) had history of small congenital nevi at the primary sites. There are some differences in the clinical features such as location and age between the skin cancer patients in our study and those in white population. The incidence of skin cancer in the two hospitals had been increasing in the 20 years (between 1981 and 2000). Both trauma and small congenital nevi are important risk factors of CMM.
    Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 11/2004; 36(5):469-72.
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    ABSTRACT: To construct and screen the human phage antibody library from a patient with melanoma. Total RNA was extracted from the peripheral blood mononuclear cells (PBMCs) of a patient with melanoma who has survived for twenty years. The heavy chain Fd fragments and kappa light chains were amplified by RT-PCR. The amplified products were cloned into phagemid vector to construct Fab antibody library. The library was panned with two kinds of melanoma cells for 4 rounds. Antibody library contained 1.2 x 10(8) different clones. Specific phage antibodies against one of the two melanoma cells were obtained after 4 rounds of panning. Specific human phage antibodies against melanoma cells were obtained.
    Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 10/2004; 20(5):588-91.
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    ABSTRACT: Human antibodies generated by phage antibody technology have been widely used in the immunotherapy of various diseases. Among the characteristics of these therapeutic antibodies, affinity is one of the most important determinants of their biological efficacy. The binding of an antibody and its corresponding antigen could be disrupted by thiocyanate solution of different concentrations, depend upon the affinity of the antibody. This mechanism has been adopted to determine the relative affinity of monoclonal or polyclonal antibodies in routine immunological practice. Correlation between the elution method and other techniques that measure the affinity such as equilibrium dialysis and biospecific interaction analysis (BIA) has been established. Here we describe the applications of the thiocyanate elution method in the determination of the relative affinity index (RAI) of phage antibodies (Phabs). Five clone antibodies, including 3 clones of anti-keratin antibodies (AK1, AK2 and AK3) and 2 clones of anti-HBsAg antibodies (HB1 and HB2) were selected to express Phabs and Fabs, and the RAI were determined by ELISA after thiocyanate elution. A HRP-conjugated anti-M13 was used as secondary antibody for Phabs and HRP-goat-anti-human Fab was used for Fabs. The affinity ranks of the Phabs were compared with that of the Fab fragments. The results showed that all the Phabs tested were tolerant to thiocyanate treatment. The relative affinity rank of 5 Phabs coincided well with that of their corresponding Fabs. We conclude that the thiocyanate elution can be used as an easy and rapid method to measure and compare the relative affinity of Phabs.
    Sheng wu gong cheng xue bao = Chinese journal of biotechnology 06/2004; 20(3):429-33.
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    ABSTRACT: Punctate palmoplantar keratodermas (PPK) is a rare autosomal dominant cutaneous disorder characterized by numerous hyperkeratotic papules that are irregularly distributed on the palms and soles. The genetic basis for this disease is unknown. We performed a genome-wide search in two Chinese families with punctate PPK to map the chromosome location of the responsible gene. We identified a locus at chromosome 8q24.13-8q24.21 with a cumulative maximum two-point LOD score of 5.41 at markers D8S1793 and D8S1774 (at recombination fraction theta=0.00). Haplotype analysis indicated that the disease gene is located within 9.20 cM region between markers D8S1804 and D8S1720. It is the first locus identified for the punctate PPK. This study provides a map location for isolation of a disease gene-causing punctate PPK.
    Journal of Investigative Dermatology 06/2004; 122(5):1121-5. · 6.19 Impact Factor
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    [Show abstract] [Hide abstract]
    ABSTRACT: Punctate palmoplantar keratodermas (PPK) is a rare autosomal dominant cutaneous disorder characterized by numerous hyperkeratotic papules that are irregularly distributed on the palms and soles. The genetic basis for this disease is unknown. We performed a genome-wide search in two Chinese families with punctate PPK to map the chromosome location of the responsible gene. We identified a locus at chromosome 8q24.13–8q24.21 with a cumulative maximum two-point LOD score of 5.41 at markers D8S1793 and D8S1774 (at recombination fraction θ=0.00). Haplotype analysis indicated that the disease gene is located within 9.20 cM region between markers D8S1804 and D8S1720. It is the first locus identified for the punctate PPK. This study provides a map location for isolation of a disease gene-causing punctate PPK.
    Journal of Investigative Dermatology 01/2004; 122(5):1121-1125. · 6.19 Impact Factor
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    ABSTRACT: To prepare the polyclonal antibody to B cell epitope domain of TRP-1 and apply it to study the immunotherapy of vitiligo and melanoma. The fusion protein PRSETA/TRP-1 was expressed in E.coli, the polyclonal antibody was prepared by immunizing a rabbit with the protein. The qulity of the antibody was identified by ELISA and Western-blot. (1)The pRSEA/TRP-1 fusion protein was correctly expressed;(2)the polyclonal antibodies to the B cell eoitope domain of TRP-1 were obtained; (3)the rabbit antiserum containing of polyclonal antibody showed high titer and possessed good binding activity to 6His-TRP1 expressed by Pichia pastoris. The polyclonal antibody could be applied to the research on B cell epitope domain of TRP-1.
    Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 06/2003; 19(3):263-5.