Xiao-Shan Zhao

Nanfang Hospital, Shengcheng, Guangdong, China

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Publications (12)5.26 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: To study the relationship between sub-health status and the health-promoting lifestyle of employees. A total of 5316 employees in a company in Guangdong were surveyed using sub-health measurement scale version 1.0 (SHMS V1.0) and the health-promoting lifestyle profile (HPLP-II). The former scale included 3 subscales of somatic sub-health, psychological sub-health and social sub-health, and the latter included 6 subscales of self-actualization, healthy responsibility, physical exercise, nutrition, interpersonal sensitivity and stress treatment. The total healthy rate was 12.86% among the employees, with 76.76% and 10.5% in sub-health and disease states. The mean scores of HPLP-II was 115.95∓21.468 in the total population surveyed, 134.23∓24.72 in healthy employees and 114.69∓19.25 in the patients. There was a significant difference in the grades of health-promoting lifestyle between sub-healthy and healthy employees (P<0.05) as well as in the scores of HPLP-II and the scores of the 6 subscales (P<0.05). An appreciable correlation was found between sub-health status and the 6 subscales, and self-realization, physical exercise and stress management showed significant inverse correlation with sub-health status. Sub-health status is related to health-promoting lifestyle, and self-realization, physical exercise and management are the protective factors that influence sub-health status.
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University 08/2013; 33(8):1203-1206.
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    ABSTRACT: To investigate the effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells (HSkMCs), HSkMCs were cultured in vitro and treated with serum from healthy and sub-healthy subjects independently. Total RNA was extracted, purified and used for reverse transcription into cDNA. This was labeled with one of two fluorescence dyes, Cy3 or Cy5, and hybridized with a Human Genome Array carrying 8064 target genes. The differentially expressed genes were selected with the aid of software. There were 86 differentially expressed genes identified between the sub-healthy subjects and the healthy individuals, of which 60 were upregulated and 26 were downregulated compared with those of the healthy individuals. These genes were mainly associated with the cell cycle, membrane channels, protein transport, energy metabolism and apoptosis. Our results suggest that serum from sub-healthy subjects with fatigue may change the gene expression profile of HSkMCs.
    Molecular Medicine Reports 12/2012; · 1.17 Impact Factor
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    ABSTRACT: The anti-fatigue effects of the Radix Rehmanniae Preparata polysaccharides (RRPP) were studied in mice. The RRPP were orally administered at doses of 50, 100 and 200 mg/kg for 4 weeks and the anti-fatigue activity was evaluated using a weight-loaded swimming test, along with the determination of serum urea nitrogen (SUN), hepatic glycogen and blood lactic acid (BLA) contents. The results showed that there was no significant difference in the body weight of mice in the three RRPP groups compared with the negative control group during initial, intermediate and terminal stages in the experiment (p>0.05). The ratio of exhausting swimming time was obviously increased 31.48% (p<0.05) and 61.51% (p<0.01) in the middle-dose group and the high-dose RRPP group, respectively. The BLA and SUN levels were decreased in middle-dose and high-dose RRPP groups (p<0.01). Hepatic glycogen level was increased in three RRPP treated groups (p<0.01). Therefore, RRPP may be responsible for the pharmacological effect of anti-fatigue of Radix Rehmanniae Preparata. The mechanism was related to the increase of the storage of hepatic glycogen and the decrease of the accumulation of SUN and BLA.
    International journal of biological macromolecules 01/2012; 50(1):59-62. · 2.37 Impact Factor
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    ABSTRACT: Objectives. To assess the efficacy and safety of Liu Jun Zi Tang (LJZT) and Xiang Sha Liu Jun Zi Tang (XSLJZT) for treating functional dyspepsia. Methods. Literature searches were carried out on Medline database, Cochrane Library, CNKI database, Chinese Biomedical Literature database, Wanfang database, and VIP database up to July 2012. Hand search for further references was conducted. Study selection, data extraction, quality assessment, and data analyses were performed according to the Cochrane standards. Results. Fifteen publications in total were suitable for inclusion. There was evidence that LJZT compared with prokinetic drugs increased symptom improvement (odds ratio 1.96, 95% CI 1.15 to 3.36). There was also evidence that XSLJZT compared with prokinetic drugs increased symptom improvement (odds ratio 2.63, 95% CI 1.72 to 4.03). No adverse events were reported in LJZT or XSLJZT group in any of these randomized controlled trials. Conclusion. LJZT and XSLJZT might be more effective compared with prokinetic drugs in the treatment of functional dyspepsia, and no side effects are identified in the included trials. However, due to poor methodological quality in the majority of included studies, the potential benefit from LJZT and XSLJZT need to be confirmed in rigorously designed, multicentre, and large-scale trials.
    Evidence-based Complementary and Alternative Medicine 01/2012; 2012:936459. · 1.72 Impact Factor
  • Min Wei, Xiao-shan Zhao, Xiao-min Sun, Jing Chen, Ren Luo
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    ABSTRACT: To study the gene expression profiles in kidney Yang deficiency in individuals with sub-health status. The subjects identified to have Kidney-yang deficiency and sub-health status were enrolled in this study with healthy subjects as the normal control. Using gene chip technique and MAS software, the gene expression profiles in kidney Yang deficiency in the subjects with sub-health status were analyzed. Characteristic profiles of the differentially expressed genes were found between the kidney Yang deficiency group and normal control group. Among the total of 75 differentially expressed genes, 22 were up-regulated (with a ratio greater than 2) and 53 were down-regulated (with a ratio less than 0.5) in kidney Yang deficiency group. Gene chip technique allows rapid and high-throughput screening for gene expression in kidney Yang deficiency and sub-health status, and a initial evidence of kidney Yang deficiency-related genes has been obtained.
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University 02/2011; 31(2):248-51.
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    ABSTRACT: To assess the reliability and validity of the Sub-health Measurement Scale Version 1.0 (SHMS V1.0). A spot trial sampling of 2000 individuals was conducted to study the test-retest reliability, Cronbach alpha coefficient, split-half reliability, contract validity, content validity and criterion-related validity of SHMS V1.0. The spot trial results indicated a test-retest reliability of SHMS V1.0 of 0.644 (P<0.001) with a Cronbach α coefficient of 0.917 and a split-half reliability of 0.831. The results showed a close correlation between each item of SHMS V1.0 and its dimension, but a low correlation between a particular item and other dimensions. The dimension score was significantly correlated to its subscale scores, but not to other subscale scores. The results of factor analysis matched the theoretical conception of SHMS V1.0. The correlation coefficient between SHMS V1.0 and SF-36 was 0.664 (P<0.001). SHMS V1.0 has a good reliability and validity, and is a reliable and valid measurement scale for sub-health evaluation.
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University 01/2011; 31(1):33-8.
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    ABSTRACT: To explore the clinical manifestation of subhealth status in teachers and medical staff. An on-the-spot investigation was conducted in 891 personnel of a medical college using a self-designed subhealth questionnaire. The diagnostic criteria for subhealth status were formulated on the basis of literature review and expert counseling. The results were analyzed by frequency analysis and multiple-factor logistic analyses. The total subhealth incidence was 57.2 %, and the clinical manifestations included many symptoms of the body, psychology and society. Multiple-factor logistic analysis revealed the most common clinical manifestations of subhealth status including ademonia, weariness, insomnia, waist and back pain, frequent sighs, eye dryness, inattention, irritability, foot and hand coldness, vulnerability to common cold etc. The clinical manifestations of sub-health status complex.
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University 05/2007; 27(4):448-9.
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    ABSTRACT: To evaluate the reliability and validity of the questionnaire we designed for subhealth status survey based on the symptoms described in traditional Chinese medicine. By on-the-spot investigation, the recovery rate of the questionnaires was 96.3% and the response rate of the items was 97.2%. Internal consistency reliability coefficient of the questionnaire was high (0.9274-0.9676), and each item was closely associated with its related factors (with Spearman coefficient mostly above 0.6 except for that for bodily symptoms in female). Factor loading was approximately consistent with the structure and content of the questionnaire. This subhealth status questionnaire can be reliable and effective.
    Nan fang yi ke da xue xue bao = Journal of Southern Medical University 03/2007; 27(2):160-3.
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    ABSTRACT: To understand the association between plasminogen activator inhibitor-1 (PAI-1) gene 4G/5G polymorphism and type 2 diabetic nephropathy (DN) in Chinese Han patients in Guangdong Province. PAI-1 gene 4G/5G polymorphism was analyzed in 26 normal individuals, 77 patients with type 2 diabetic nephropathy and 70 type 2 diabetic patients without nephropathy (DNN) by polymerase chain reaction-restriction fragment length polymorphism. The 4G/4G genotype frequencies of PAI-1 gene were higher in DN than in DNN patients (0.390 vs 0.171, chi(2)=13.008, P<0.01), with the odds ratio (OR) of 1.447 (95% confidence interval: 0.533-3.934, P<0.05) in comparison with 5G/5G genotypes between the 2 patient groups. The 4G allele frequencies of PAI-1 gene were similar in DN and DNN groups (0.57 vs 0.51, chi(2)=1.22, P>0.05). The PAI-1 4G/4G genotype is associated with increased risk for type 2 DN the Chinese Han population in Guangdong Province, and type 2 diabetic patients with 4G/4G PAI-1 genotype are more likely to develop DN than those with 5G/5G PAI-1 genotype.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 09/2004; 24(8):904-7.
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    ABSTRACT: To explore the regulatory mechanism of matrix metalloproteinase-9 (MMP-9) gene expression induced by phorbol 12-myristate 13-acetate (PMA) in cytotrophoblastic cells. Enzyme-linked immunosorbent assay (ELISA) was used to determine the kinase activity, and MMP-9 gene expression was detected by semi-quantitative reverse transcription (RT)-PCR in the cytotrophoblastic cells. Cytotrophoblastic cells treated with PMA showed markedly increased MMP-9 mRNA level. PMA treatment caused an increase in extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (p38 MAPK) activities. Both SB203580 (specific inhibitor of the p38 MAPK) and PD98059 (specific inhibitor of the ERK), when preincubated for 30 min with the cytotrophoblastic cells, substantially reduced MMP-9 mRNA accumulation in PMA-primed cells. However, neither SB203580 nor PD98059 used alone, or their combination, was able to completely inhibit MMP-9 mRNA expression. Both p38 MAPK and ERK pathways are involved in the regulation of MMP-9 gene expression in the cytotrophoblastic cells induced by PMA, and both signaling pathways are indispensable for full activation of MMP-9 gene expression.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 04/2004; 24(3):282-5.
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    ABSTRACT: To investigate the role of p38 mitogen-activated protein kinase (MAPK) signal transduction pathways in regulating the in vitro invasion of JAR human choriocarcinoma cells induced by phorbol 12-myristate 13-acetate (PMA). ELISA was used to detect the kinase activity of the JAR cells in response to PMA stimulation, and the in vitro invasion capabilities of the stimulated cells were observed using transwell assay. Changes in the proliferation and activity of the JAR cells cultured in vitro following PMA treatment were also observed by MTT assay. p38 MAPK was activated dose-dependently in JAR cells upon the stimulation by PMA, which significantly enhanced the in vitro invasion of the JAR cells, while treatment of the cells with SB203580, a specific inhibitor of p38 MAPK, inhibited the invasion of the cells. The growth of the cells, as observed from the growth curves, was not affected by the treatment of PMA and/or SB203580. Activation of p38 MAPK signal transduction pathway may enhance the invasion capability of JAR cells, and p38 MAPK inhibition may therefore yield new possibility to control the invasion of choriocarcinoma.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 09/2003; 23(8):792-4.
  • Xiao-Shan Zhao, Ren Luo
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    ABSTRACT: Study of kidney deficiency syndrome may utilize the modern molecular biology technique, such as gene chip, representational difference analysis (RDA) and genes sequence analysis, to clone the different genes of kidney deficiency syndrome from single TCM syndrome type of the disease, to investigate the inner connection between the various phenotypes of kidney deficiency syndrome and their genes expression pattern and their function products, then to definite the related genes of kidney deficiency syndrome. The research method may also be used for the study of other TCM syndrome types.
    Journal of Chinese Integrative Medicine 06/2003; 1(1):18-20.

Publication Stats

19 Citations
5.26 Total Impact Points

Institutions

  • 2003–2013
    • Nanfang Hospital
      Shengcheng, Guangdong, China
  • 2012
    • Guangzhou University of Traditional Chinese Medicine
      Shengcheng, Guangdong, China
    • Southern Medical University
      • School of Traditional Chinese Medicine
      Shengcheng, Guangdong, China
  • 2011
    • Fujian University of Traditional Chinese Medicine
      Min-hou, Fujian, China