D Rondina

Universidade Estadual do Ceará, Ceará, Ceará, Brazil

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Publications (38)47.41 Total impact

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    ABSTRACT: The aim of this study was to determine whether the consumption of detoxified castor meal (DCM) by goats over a long period of time affects mRNA levels in oocytes, and in mural granulosa and cumulus cells. A total of 41 adult does were supplemented (DCM group, n=21) or not (control group, n=20) with detoxified castor meal (DCM) for a period of 500 days. Then, 13 and 12 does were randomly selected for slaughter from the DCM and control treatments groups, respectively, for the determination of the number of visible ovarian follicles, retrieved cumulus-oocyte complexes (COCs), and viable and non-viable oocytes. The relative expression levels for distinct genes were determined by quantitative PCR in viable immature oocytes prior to in vitro maturation (IVM), in oocytes attaining or not the metaphase stage after IVM, as well as in granulosa cells obtained upon oocyte collection, and in cumulus cells obtained after IVM. The number of follicles ≥4mm did not differ between treatments (overall mean 23.3±2.0) and no significant differences were observed in the recovery of viable, non-viable, or total mean numbers of oocytes (control group: 44.7±4.6, DCM group: 54.9±5.9, respectively) between control and DCM fed goats. The maturation rate was significantly higher for control than DCM oocytes (58.0% vs. 45.3%; P<0.05). The mRNA levels in immature COC for controls were significantly higher for GLUT1 and lower for HSP70 (P<0.05) than for DCM. Following maturation, MII oocytes from both treatments had mRNA levels that were significantly higher for GDF9 and lower for BMP15 than for NC oocytes (P<0.05). In cumulus cells, the mRNA levels were significantly higher for LHR, FSHR, LeptinR, and IGF1, and lower for MnSOD in the control group compared with the DCM group (P<0.05). In conclusion, the inclusion of DCM in goat feed for long periods of time changed gene expression in immature oocytes and in cumulus cells. This was reflected by a decrease in the in vitro oocyte maturation rate.
    Animal reproduction science. 06/2014;
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    ABSTRACT: Lipid-rich and energy-dense diets can have significant effects on the reproductive physiology, including the ovarian function and fertility. The aim of this study was to assess the effect of cashew nut bran supplementation as a lipid source on follicle development, plasma and intrafollicular concentrations of cholesterol, and developmental competence of in vitro-matured goat oocytes. The inclusion of cashew nut bran as 24% of the goats' diet for 28 days increased the percentage and number of degenerated oocytes compared with the control (P < 0.05), and also the plasma cholesterol levels and the proportion of grade IV oocytes compared with all other treatments (P < 0.05). Moreover, a significant reduction was observed in the proportion of viable oocytes compared with the control and in the percentage of grade II oocytes compared with all other treatments (P < 0.05). Oocyte maturation, cleavage, and blastocyst rates after parthenogenetic activation of viable oocytes were not affected by the type of diet. In conclusion, the inclusion of cashew nut bran as 24% of the diet of adult goats for 28 days changed plasma cholesterol levels and reduced the proportion of viable immature oocytes; however, the 12% and 24% diet supplementations with cashew nut bran did not interfere with competence of resulting viable oocytes to reach the metaphase II stage after IVM, and to develop after parthenogenetic activation.
    Theriogenology 04/2014; · 2.08 Impact Factor
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    ABSTRACT: This study aimed to assess the effects of dietary supplementation with crude glycerin on embryo quality and expression profile of genes from superovulated goats. Twenty-four hours (1 day) and 72 hours (3 days) after mating animals received an oral drench of 200 mL saline solution (Control group), 100 mL glycerol (100 mL group) or 200 mL glycerol (200 mL group). Both insulin and glucose levels were measured after glycerol drenching. The relative expression of genes (Glut-1, IGF-1, Hsp70, Sod and Bax) in grade I and II embryos from groups was analyzed by sensitive TaqMan qPCR. Administration of crude glycerin raised insulin and glucose (p < 0.05) plasma concentrations. Similar rates of superovulatory response (94.12%; 16/17) and recovery of transferable embryos (N embryo grades 1, 2 and 3/N ovulation), (44.69 ± 6.83%; 100/224) were obtained. The 200 mL group showed a greater proportion of transferable embryos compared to the degenerated embryos (8.83 ± 2.30 vs. 1.17 ± 0.83, p < 0.05), that achieved values 2-fold higher than control group (4.80 ± 2.30 vs. 0.20 ± 0.20, p < 0.05) and 100 mL group (3.83 ± 1.25 vs. 1.17 ± 0.48, p > 0.05). A higher relative abundance of IGF-1 transcripts was observed in 200 mL group. Also none differences were observed for transcripts of apoptotic (Bax) and stress (Hsp70) genes. In conclusion, enhancement of circulating glucose and insulin generated from drenching crude glycerin after mating was associated with higher IGF-I expression and increased proportion of transferable embryos.
    Small Ruminant Research 01/2014; · 1.12 Impact Factor
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    ABSTRACT: Lipid-rich and energy-dense diets can have significant effects on the reproductive physiology, including the ovarian function and fertility. The aim of this study was to assess the effect of cashew nut bran supplementation as a lipid source on follicle development, plasma and intrafollicular concentrations of cholesterol, and developmental competence of in vitro-matured goat oocytes. The inclusion of cashew nut bran as 24% of the goats’ diet for 28 days increased the percentage and number of degenerated oocytes compared with the control (p<0.05), as well as the plasma cholesterol levels and the proportion of Grade IV oocytes compared with all other treatments (p<0.05). Moreover, a significant reduction was observed in the proportion of viable oocytes compared with the control and in the percentage of Grade II oocytes compared with all other treatments (p<0.05). Oocyte maturation, cleavage and blastocyst rates after parthenogenetic activation of viable oocytes were not affected by the type of diet. In conclusion, the inclusion of cashew nut bran as 24% of the diet of adult goats for 28 days changed plasma cholesterol levels and reduced the proportion of viable immature oocytes; however, the 12% and 24% diet supplementations with cashew nut bran did not interfere with competence of resulting viable oocytes to reach the metaphase II stage after in vitro maturation, and to develop after parthenogenetic activation.
    Theriogenology 01/2014; · 2.08 Impact Factor
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    ABSTRACT: In the present study, we evaluated the effect of long-term ingestion of detoxified castor meal on in vitro development of sheep preantral follicles as well as on the developmental competence of oocytes from antral follicles. Ovarian fragments were cultured for one or seven days and further analyzed by histology and fluorescence microscopy (experiment 1). The cumulus oocyte complexes (COCs) obtained from antral follicles were matured in vitro and activated by parthenogenesis or fertilization in vitro (experiment 2). Even after 1 day of culture, in both tested groups, a significant reduction in the percentage of primordial follicles and a concomitant increase in the percentage of intermediate follicles were observed when compared to non cultured tissue. After 7 days of culture, the percentage of primary follicles in both tested groups was significantly higher when compared to the non cultured tissue or the tissue cultured for 1 day (P < 0.05). The number of in vitro embryos produced was similar between the tested groups. However, for those animals fed with detoxified castor meal, the parthenogenesis method resulted in a higher number of embryos (morulae) when compared to in vitro fertilization (IVF) activation. In conclusion, detoxified castor meal can be used as an alternative protein source without affecting the ovine preantral and antral folliculogenesis.
    Small Ruminant Research 01/2013; 113(s 2–3):353–359. · 1.12 Impact Factor
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    ABSTRACT: Thirty-nine nulliparous and cyclic free-ranging Anglo-nubian goats were synchronized using 60 mg MAP intravaginal sponges for 10 days and 50 μg cloprostenol treatment, 48 h before sponge removal (SR). Goats were allocated to 3 groups with 13 animals in each group. The treatment groups, Insulin 0.14 and Insulin 0.20 were treated subcutaneously, for 3 consecutive days – beginning 48 h before SR, with 0.14 IU/kg BW/day or 0.2 IU/kg BW/day of a long-acting insulin, respectively. The Control group received a 0.5 ml saline solution (s.c.). The goats were monitored for the occurrence of estrus and mated at the onset of estrus and 24 h later. Transrectal ultrasonography was used to monitor the ovarian follicular dynamics, ovulation rate and diagnose pregnancy. Blood samples were collected from 48 h before SR to the 12th day of pregnancy for plasma insulin determinations. Insulin administration exhibited an increase (p < 0.05) in animals with plasma insulineamia. Insulin treated goats recorded a higher (p < 0.05) ovulation rate, when compared to saline control treated goats (1.7 ± 0.23 vs 1.0 ± 0.21). Nulliparous does from the Insulin 0.14 group recorded large follicles at 0, 12 and 18 h of the estrous period (p < 0.05), a more efficient synchronization of ovulation (p < 0.05) and larger size CLs (p < 0.05). It was concluded that administration in insulin induced a positive effect on ovarian response in nulliparous goats. Furthermore, in these animals the reproductive performance appeared to be independent of the increase of insulin dosage.
    Small Ruminant Research 06/2012; 105(s 1–3):282–285. · 1.12 Impact Factor
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    Arquivo Brasileiro de Medicina Veterinária e Zootecnia 02/2011; 63(1):171-179. · 0.27 Impact Factor
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    ABSTRACT: In order to evaluate the performance and carcass characteristics were used 14 whole lambs, fed with diets containing concentrate and sorghum silage or dehydrated cashew apple bagasse (DCAP) as source of roughage. The animals had an average initial body weight of 12 kg and were slaughtered when they reached 25 kg of live weight. We used a completely randomized design with two diets and seven replicates. The feed conversion of the group fed with DCAP (8.08 ± 0.32) was better (p
    Archivos de Zootecnia 01/2011; 60(231):777-786.
  • Reproduction Fertility and Development - REPROD FERT DEVELOP. 01/2010; 22(1).
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    ABSTRACT: Ovarian cortical fragments from five adult ewes were in vitro cultured for 1, 3 or 5 days in the presence of minimum essential medium either supplemented or not by follicle-stimulating hormone (FSH) (100 ng/ml) or indole-3-acetic acid (IAA) (10, 20, 40 or 100 ng/ml), alone or in combination. After in vitro culture, ovarian fragments were submitted to follicular isolation and viability test was performed using trypan blue. Addition of IAA (10 ng/ml) to a free-FSH medium resulted in the highest percentages of viable follicles, but was progressively deleterious in higher concentrations (20, 40 and 100 ng/ml) if in absence of FSH. Follicular development was observed only when FSH was added to an IAA-free medium. In conclusion, IAA at a concentration of 10 ng/ml increases follicular survival in vitro. However, at high concentrations (20, 40 or 100 ng/ml), this auxin may be deleterious to preantral follicles, the addition of FSH to the medium being necessary.
    Zygote 08/2009; 18(1):89-92. · 1.50 Impact Factor
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    ABSTRACT: RESUMO O objetivo deste trabalho foi determinar a relação da idade gestacional com a mensuração do embrião/ feto em cabras, através da ultrassonografia, em tempo real. Oito cabras nulíparas da raça Anglo-Nubiana foram submetidas a exames ultrassonográficos, a partir do 25° dia após a primeira monta, utilizando um equipamento de ultrassom, acoplado a transdutor linear (6/8 MHz) e convexo (5 MHz). Nas imagens gravadas, o diâmetro da vesícula embrionária (DVE) e o comprimento crânio-caudal (CCC), além do comprimento da cabeça (CC), do diâmetro biparietal (DBP), do tórax (DT), do cordão umbilical (DCU), do eixo longo do coração (ELC), do eixo curto do coração (ECC) e dos placentomas do embrião/feto foram mensurados, usando um programa computacional. A relação entre a idade gestacional e cada parâmetro mensurado por ultrassonografia foi expressa em equações de linha reta e mostrada como regressão linear, usando o programa Statistica 7.0 com p < 0,05. As médias dos valores encontrados foram correlacionadas com a idade fetal. Os coeficientes de correlação foram DVE (r = 0,97), CCC (r = 0,97), CC (r = 0,94), DBP (r = 0,94), DT (r = 0,99), DCU (r = 0,97), ELC (r = 0,98), ECC (r = 0,93) e do placentoma (r = 0,95). Adicionalmente, o tamanho do embrião aumentou, desde sua primeira visualização, até o fim do estudo. Em conclusão, o método de ultrassonografia mostra-se um meio confiável para estimar a idade gestacional, a partir da mensuração embrionária/fetal, uma vez que o aumento no valor destes parâmetros segue um padrão linear de regressão. ABSTRACT The aim of this study was to determine the relation of gestational age with embryonic/fetal measurements in goats through the real-time ultrasound method. Ultrasonographic examinations began on the 25 th day after first mating in eight Anglo-Nubian nulliparous goats, using a ultrasound scanner fitted to 6/8 MHz linear-array and 3.5/5 MHz convex probe. On recorded scanning images, the embryonic vesicle diameter (EVD) and crown-rump length (CRL), moreover head length (HL), bi-parietal diameter (BPD), thoracic diameter (TD), umbilical cord diameter (UCD), long axis of heart (LAH) and short axis of heart (SAH) and placentomes of embryo/fetus were measured using
    02/2009;
  • Salles, MGF, Souza, CEA, D Rondina, AA Moura, AA Araujo
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    ABSTRACT: This study aimed to assess the effects of ambient temperature (AT) and relative humidity (RH) at different periods of the year (rainy, rainy-dry transition, dry and dry-rainy transition) on physiological parameters of Saanen bucks in tropical climate. We used nine bucks, three times a week in the morning (9:00 AM) and afternoon (1:00 PM) had collected their physiological data of rectal temperature (RT), respiratory rate (RR) and heart rate (HR). The climatic data of AT and RH were recorded for calculating the index of temperature and humidity (THI) and evaluation of thermal comfort. Data were expressed as mean and standard error and evaluated by ANOVA and means compared by Tukey test at 5% of probability error. Based on the results, we conclude that Saanen bucks are susceptible to heat stress at any period of year and the transition dry-rainy period it the most critical for occurrence of heat stress. The afternoon shift, whatever the period of the year, is the moment of environmental discomfort for the animals.
    Ciência Animal. 01/2009; 19:19-28.
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    ABSTRACT: Seventeen adult and cyclic Moxoto goats were synchronized using 60 mg MPA vaginal sponge for 11 days and 50 mug cloprostenol, 48 h before sponge removal, and superovulated with 120 mg pFSH i.m. in decreasing doses at 12 h intervals for three consecutive days. In seven goats, 0.2 IU/kg BW/day of long acting insulin was subcutaneously injected at same time as pFSH, and in the other five goats, the same dose of insulin was injected for three consecutive days starting 24 h after mating. Finally, five goats were supplemented with an oral dose of 80 ml/goat/day of propylene glycol continuously during the experiment. The animals were flushed at 7 days after mating and the embryos were classified based on International Embryo Transfer Society criteria. Blood samples were collected every 3 days for insulin assay. Administration of insulin raised the insulin levels of the goats (p < 0.05), whereas in the group treated with propylene glycol, insulin rate was different only between FSH treatment and after mating (p < 0.05). Similar rates of recovery for total (80.05 +/- 9.78%) or transferable structures (61.03 +/- 15.13%) were obtained. Treatment was not influenced (p > 0.05) by responsiveness to superovulation, which averaged 64%. By contrast, insulin treatments were shown to increase the number of embryos considered excellent with respect to goats supplemented with propylene glycol (p < 0.05). When insulin was given before mating, a strong relationship (r = 0. 90) (p < 0.05) between number of transferable embryo and ovulations was observed in the animals. In conclusion, superovulated goats treated with low doses of exogenous insulin resulted in an enhancement in embryo quality, which was related to changes in circulating insulin concentrations.
    Reproduction in Domestic Animals 05/2008; 43(2):218-21. · 1.39 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the efficiency of 1 M dimethylsulphoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH) and glycerol (GLY) to cryopreserve primordial follicles. The first evaluation was performed soon after cryopreservation and the second evaluation after 4 days of in vitro culture, using the cryoprotectants that allowed the higher results (higher follicular survival rate) after cryopreservation. The results after follicular isolation (control) and cryopreservation using 1 M DMSO, EG, PROH and GLY showed that the mean number (+/- SEM) of live follicles per millilitre was 3204 (100%) +/- 319.27, 2798 (87%) +/- 239.14, 2492 (78%) +/- 345.8, 448 (14%) +/- 46.3 and 208 (7%) +/- 75.26, respectively. Higher follicular survival was reported when DMSO and EG were used. Control follicles and follicles cryopreserved with these two cryoprotectants were cultured and the percentage of follicular survival was 55% (control), 42% (EG) and 34% (DMSO). Similar results were found between control and follicles cryopreserved with EG. In conclusion, 1 M EG is the most effective cryoprotectant to preserve primordial follicles isolated from ovaries of sheep.
    Reproduction in Domestic Animals 03/2007; 42(1):53-7. · 1.39 Impact Factor
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    ABSTRACT: To determine the behavior of isolated primordial follicles that were exposed to different concentrations of dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH), and glycerol (GLY). Isolated primordial follicles were exposed to the cryoprotectant (CPA) solution and photographed to calculate their volume at different periods of exposure. Laboratorio Renzo Giuliani, University of Florence, Italy. Lambs, 30-40 days old. Isolation of primordial follicles and subsequent exposure to CPA. Follicular volume. At 2 minutes of CPA exposure, all follicles appeared to be shrunken. At approximately 5 minutes, shrinkage ceased, and follicles started to swell, absorbing the CPA and water to maintain osmotic equilibrium. When DMSO was tested, follicular dehydration in all concentrations did not exceed 17%; with PROH and EG, it reached 33% and 27%, respectively. The highest degree of dehydration (48%) was seen with GLY. In almost all tested concentrations, follicular shrinkage occurred up to 5 minutes. Volume changes in isolated primordial follicles can fluctuate according to the CPA used and its concentration.
    Fertility and sterility 05/2006; 85 Suppl 1:1077-81. · 3.97 Impact Factor
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    ABSTRACT: The objective of the present study was to evaluate the effects of cryopreservation of goat preantral follicles, in situ and isolated, using dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Follicles were cryopreserved and cul-tured in vitro, and follicle viability was assessed by Trypan Blue staining before and after culture. The viability of follicles cryopreserved in situ using 1.5 M DMSO (64% 5.3 and 60% 7.4) or 3.0 M EG (58% 3.7 and 45% 4.4) after days 1 and 5, respectively, of culture was lower (p 0.05) than before culture (84% 6.9). Similar results were obtained with isolated follicles cryopreserved in 1.5 M EG after 1 (51% 6.5) and 5 (63% 6.6) days of cul-ture. The viability of fresh and cryopreserved follicles in situ was similar on days 0, 1, and 5 of culture (P 0.05). For isolated follicles, the percentage of viable fresh follicles was higher than follicles cryopreserved in EG on days 1 and 5 of culture, and in DMSO on day 1 of culture. Follicle diameter was not altered during culture, both for fresh and cryopreserved follicles and when follicles were cultured in situ or isolated. In conclusion, caprine pre-antral follicles were successfully cryopreserved, especially in situ, and follicle viability after isolation was simi-lar between fresh and cryopreserved follicles after 1 day of culture, when DMSO was used.
    Cell Preservation Technology 01/2006; 4(4):290-298. · 1.03 Impact Factor
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    ABSTRACT: In order to evaluate embryo production in Morada Nova (white variety) ewes superovulated with porcine follicle-stimulating hormone, 20 cycling ewes were used as embryo donors and allocated into two groups according to age: group 1 (ewes aged 1-2 years; n = 9) or group 2 (ewes aged 3-4 years; n = 11). Embryo recovery was performed by laparotomy 5-6 days after oestrus. The evaluation of embryos was made under stereomicroscope according to International Embryo Transfer Society rules. The overall recovery rate was 64.6% (5.0 +/- 0.7 structures per ewe) and 86.3% of the recovered structures were fertilized. Group 1 was superior (p < 0.05) to group 2 according to recovered (6.6 +/- 0.9 vs 3.6 +/- 0.8) and fertilized structures (5.6 +/- 1.1 vs. 3.5 +/- 0.7) per ewe. In conclusion, the ovarian response and the embryo production in Morada Nova (white variety) sheep subjected to a standard superovulation treatment were considered satisfactory. In addition, the use of multiple ovulation and embryo transfer in younger ewes ( < or = 2 years old) of this sheep breed appears to be an efficient tool to accelerate the preservation of the Morada Nova (white variety) breed, since younger ewes are as responsive as older ones.
    Tropical Animal Health and Production 01/2006; 38(7-8):555-61. · 1.09 Impact Factor
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    ABSTRACT: The objective of the present study was to evaluate the effects of cryopreservation of goat preantral follicles, in situ and isolated, using dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Follicles were cryopreserved and cul-tured in vitro, and follicle viability was assessed by Trypan Blue staining before and after culture. The viability of follicles cryopreserved in situ using 1.5 M DMSO (64% 5.3 and 60% 7.4) or 3.0 M EG (58% 3.7 and 45% 4.4) after days 1 and 5, respectively, of culture was lower (p 0.05) than before culture (84% 6.9). Similar results were obtained with isolated follicles cryopreserved in 1.5 M EG after 1 (51% 6.5) and 5 (63% 6.6) days of cul-ture. The viability of fresh and cryopreserved follicles in situ was similar on days 0, 1, and 5 of culture (P 0.05). For isolated follicles, the percentage of viable fresh follicles was higher than follicles cryopreserved in EG on days 1 and 5 of culture, and in DMSO on day 1 of culture. Follicle diameter was not altered during culture, both for fresh and cryopreserved follicles and when follicles were cultured in situ or isolated. In conclusion, caprine pre-antral follicles were successfully cryopreserved, especially in situ, and follicle viability after isolation was simi-lar between fresh and cryopreserved follicles after 1 day of culture, when DMSO was used.
    Biopreservation and Biobanking 01/2006; · 1.50 Impact Factor
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    ABSTRACT: Sixteen local adult goats were submitted for 9 weeks to 2.09 (high group) and 0.54 (low group) x dietary maintenance respectively. During the experimental period, goats were weighed, oestrus was detected and plasma insulin, urea, non-esterified fatty acids and progesterone concentrations were assessed. At the end of the experiment, ovarian small follicles population was studied by histological analysis. Final weight loss in low group was 18.37 +/- 2.02%, whereas weight gain of high group was 13.84 +/- 2.70%. Insulin and urea were lower in low group, while non-esterified fatty acids were significantly higher. A lower number of fasted goats was in oestrus or ovulated and an extended length of oestrus (p < 0.05) and a higher frequency of short or long cycles (p < 0.05) were also observed. Fed animals showed heavier ovaries (p < 0.01) and a lower number of primordial follicles (p < 0.05). In restricted goats a significant qualitative alteration of follicle classes involved in the initiation process of primordial pool was found. In this phase, granulosa thickness and oocyte size were the most affected (p < 0.01). However in small follicles beyond the primary stage no differences were found between the groups in either number or qualitative characteristics (p > 0.05). Collectively, these results indicate that opposite dietary intakes for a medium period induce a composite reproductive response in goats and can regulate the early onset of follicle growth.
    Reproduction in Domestic Animals 12/2005; 40(6):548-52. · 1.39 Impact Factor
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    Livestock Production Science 11/2005; 97(s 2–3):293–294.

Publication Stats

127 Citations
47.41 Total Impact Points

Institutions

  • 2001–2012
    • Universidade Estadual do Ceará
      • Faculty of Veterinary Medicine
      Ceará, Ceará, Brazil
  • 2004–2006
    • A Faculdade de Direito de Santa Maria
      Santa Maria da Boca do Monte, Rio Grande do Sul, Brazil
  • 2005
    • Universidade Estadual do Sudoeste da Bahia
      Conquista, Estado de Bahía, Brazil
  • 2003
    • University of Florence
      Florens, Tuscany, Italy