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ABSTRACT: Lugol's iodine staining (Lugol-staining) has been widely used to detect malignant changes in the cervix uteri and esophagus. However, pathological and histochemical changes in the border between Lugol-stained and -unstained areas in oral epithelial dysplastia and malignant lesions are not well understood. We examined the histological appearance of 20 cases of epithelial dysplasia surrounding squamous cell carcinoma using HE and PAS staining in the borders between Lugol-stained and -unstained areas. Subsequently, differences in the expression of cytokeratin 13 (CK13), an epithelium differentiation marker, and Ki-67, a cell proliferative marker, in those areas were investigated by immunohistochemistry. Lugol-stained areas of all cases showed mild dysplasia or normal epithelium appearance, while Lugol-unstained areas in most cases were diagnosed as moderate/severe dysplasia and carcinoma in situ. PAS reactions were limited or not found in the Lugol-unstained areas as compared to intense positivity in Lugol-stained areas. CK13 and Ki-67 protein expression was significantly different between Lugol-stained and -unstained areas. It was confirmed that epithelia showing precancerous or cancerous features were detected as Lugol-unstained boundary areas. A reduction in glycogen production caused by alterations of cell differentiation and proliferation associated with malignant changes may result in a lack of Lugol-staining.
Oncology Reports 07/2010; 24(1):9-14. · 1.84 Impact Factor
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ABSTRACT: Our aim was to clarify the association of human papillomavirus (HPV) with oral carcinogenesis, especially its early stage.
Tissue specimens of normal mucosa, epithelial dysplasia, oral squamous cell carcinoma (OSCC), and OSCC cell lines were examined for the presence of HPV-16 and HPV-18 E6 DNA by means of the polymerase chain reaction test.
The detection rate of HPV-16 in epithelial dysplasia (31/51) was higher than that in normal mucosa (16/44) and in OSCC (30/86) and was statistically different from that in OSCC. The cases that progressed from epithelial dysplasia to carcinoma showed a significantly higher HPV-16 detection rate than the other cases in both epithelial dysplasia and OSCC. HPV-16 and HPV-18 were detected only at early passages of 2 of 10 OSCC cell lines.
These results strongly suggest that HPV-16 may be involved in the early stages of the development of some oral carcinomas.
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 06/2003; 95(5):594-600. · 1.46 Impact Factor
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Journal of Oral and Maxillofacial Surgery 04/2003; 61(3):409. · 1.64 Impact Factor
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ABSTRACT: In the present study, mutations or protein expression of p53, bcl-2 and bax are reported in 13 non-Hodgkin’s B-cell lymphomas of the head and neck. Nine of 13 cases (69.2%) expressed p53 protein and 7 (53.8%) showed gene mutations. On comparing the results of immunostaining and SSCP analysis, there were two cases showing discrepancies between the p53 protein expression and gene mutations. Bcl-2 protein expression was observed in only two cases of follicular lymphoma. On the other hand, bax protein was detected in all 13 cases. One case with mutated bax gene showed a lower expression with a reduced frequency of bax-positive cells than the other 12. These results suggest that inactivation of p53 may be closely related to development and/or progression of these NHLs, and that bcl-2 and bax protein may not have a role.
Journal of Oral Pathology and Medicine 01/2002; 29(4):180 - 185. · 1.63 Impact Factor
