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ABSTRACT: To investigate the expression of casein kinase 2β in esophageal carcinoma tissues and analyze its clinical significance.
The expression of CK2β in a tissue chip containing 60 normal esophageal mucosa and 60 colorectal cancer specimens were detected immunohistochemically. Ten human esophageal carcinoma and adjacent normal tissues were examined for the expression of CK2β protein and mRNA using Western blotting and real-time quantitative PCR, respectively.
A strong expression of CK2β was found in 85.71% of the esophageal cancer tissues; 1.79% of the cancer tissues were negative for CK2β expression, and 1.79% and 10.71% of the cancer tissues were weakly and moderately positive, respectively. In the normal mucosal tissues, 96.67% of the tissues were negative for CK2β and 3.33% showed weak CK2β expression, showing a significant difference in the distribution of CK2β between normal and esophageal carcinoma tissues (P<0.001). The expression level of CK2β in esophageal cancers was associated with the pathological stage (TNM) (P=0.010). Western blotting and real-time quantitative PCR also confirmed an increased CK2β expression in the esophageal cancer tissues.
The high expression of protein kinase CK2β is closely related to the carcinogenesis and malignancy of esophageal cancer.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 10/2012; 32(10):1491-4.
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ABSTRACT: To investigate the expression of casein kinase 2β (ck2β) in colorectal cancer in relation to the metastatic ability of the cancer cells.
The expression of ck2β in 46 normal colorectal mucosa, 20 colorectal adenomas and 66 colorectal cancers were detected immunohistochemically. In colorectal cancer cells, Ck2β protein expression was knockdowned by RNA interference using ck2β-specific small interfering RNA (siRNA) and the interference efficiency was assessed by Western blotting. The effect of ck2β gene knockdown on the proliferation of the colorectal cancer cells was tested with colony formation assay, and the changes in the invasive ability of the cells were observed using Transwell chamber assay.
Negative or weak ck2β expression was detected in normal colorectal mucosa, with nuclear positivity in 8.7% and cytoplasmic positivity in 13.0% of the cases. Colorectal adenomas showed moderate ck2β expression, with 60% cases showing positivity in the cell nuclei and 40% in the cytoplasm. In colorectal cancers, significantly stronger expression of ck2β was found than that in colorectal adenomas and normal colorectal mucosa (P<0.05), and 75.8% cases showed positivity in the cell nuclei and 62.1% showed cytoplasmic positivity; the expression of ck2β protein in colorectal cancers with lymph node metastasis was even higher (P<0.05). Ck2β knockdown obviously inhibited the proliferation and invasiveness of colorectal cancer cells in vitro.
The high expression of ck2β in colorectal cancer is closely correlated to the carcinogenesis and metastasis of the tumor.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 04/2011; 31(4):628-32.
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ABSTRACT: To investigate the differential expression of microRNAs between human hepatocellular carcinoma (HCC) and liver cirrhosis (LC).
The total RNA was extracted from 25 pairs of HCC and LC tissues. microRNA microarray was used to analyze the microRNA expression profiles, and validation of the microarray results was carried out by real-time quantitative RT-PCR (qRT-PCR).
Three microRNAs exhibited higher expression in the HCC samples than in the LC samples. In comparison with the LC samples, the HCC samples showed down-regulated expressions of 9 microRNAs. qRT-PCR verified that has-miR-122a, has-miR-199a, and has-miR-199b were downregulated in HCC, which was in agreement with the microarray results.
HCC and LC samples have significantly different microRNA expression profiles. These differentially expressed microRNAs may be involved in the pathogensis of HCC.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 05/2010; 30(5):976-7.
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ABSTRACT: To investigate the effect of protein kinase CK2 gene silencing on the radiosensitization in human nasopharyngeal carcinoma (NPC) cells and its possible mechanism.
RNA interference (RNAi) technique was used to down-regulate the protein kinase CK2alpha expression in 5-8F cells, and clonogenic assay was employed to observe the changes in the radiosensitivity of the cells. DNA double-strand break was assessed by immunofluorescence staining of gamma-H2AX foci, and the cell apoptosis was examined using Annexin V-FITC/PI double-staining flow cytometry.
CK2alpha protein was successfully silenced by siRNA. CK2alpha knockdown significantly decreased the clonogenic activity and increased the radiosensitivity of the NPC cells. After a 15-min exposure of the cells to 1 Gy radiation, significant difference occurred in the gamma-H2AX foci between CK2alpha knockdown cells and the control cells (P<0.01). CK2alpha silencing significantly increased the cell apoptosis after the exposure (P<0.01).
Protein kinase CK2 plays an important role in the radiosensitivity of the NPC cells, and suppression of its expression might be a potential therapeutic approach of cancer.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 08/2009; 29(8):1551-3.
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ABSTRACT: To study the characters of chronic rhinosinusitis in patients with irradiated nasopharyngeal carcinoma.
There were 65 cases of chronic rhinosinusitis after irradiated nasopharyngeal carcinoma (NPC, experimental group) and 65 cases of common chronic rhinosinusitis (CRS, control group) in the study. The visual analogue scale (VAS) was used to evaluate the intensity of subjective symptoms. Endoscopic finding was recorded and CT results were evaluated by Lund-Mackay scoring system.
As to the VAS, nasal secretion was significantly more severe in experimental group (7.86+/-1.62), compared with control group (5.12+/-1.32, t=10.541, P<0.01). As to endoscopic finding, middle nasal meatus were clean in 35 (53.8%) cases in experimental group, and 23 cases (35.4%) in control group (chi2=4.483, P<0.05). CT score was (7.03+/-4.63) in experiment group, and (11.42+/-3.32) in control group (t=-6.207, P<0.05). The main reason lays in lower CT score and lower involved rate of ostiomeatal complex, frontal sinus, maxillary sinus, anterior ethmoid sinus.
The characters of chronic rhinosinusitis in patients with irradiated nasopharyngeal carcinoma is quite different from the common CRS and different therapeutic measures should be taken.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 04/2009; 44(4):307-10.
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ABSTRACT: To study the pathological basis of radiological reaction types of radiation-induced liver disease on multiphasic CT scans.
Three pigs (tagged with A, B, and C) were subjected to single-dose radiation of 40, 40 and 30 Gy on the right or left lobe of the liver, respectively. At 42, 56, 133, and 168 days after irradiation, all pigs were examined with non-enhanced scan and contrast-enhanced scans at different time points after contrast injection. Hounsfield units (HU) were measured in each CT study to evaluate the density of irradiated and non-irradiated liver tissue to determine the reaction type. Liver tissues in the irradiation area obtained by needle biopsy with CT guidance were examined with electron microscopy, and specimens of the tissue corresponding to the region of interest on CT were obtained from necropsies for pathological examination.
Radiologically, the 3 pig models presented with 3 reaction types on the multiphasic CT scans on days 133, 56, and 168 after radiation, respectively. Type 1 presented constant low-density change in all phases, the pathological basis of which was radiation hepatitis; type 2 showed pre-contrast phase isodense, arterial phase hyperdense, portal phase isodense and later phase hyperdense changes; type 3 was characterized by pre-contrast phase isodense, arterial phase hyperdense, portal phase hypodense and later phase hyperdense changes. The pathological basis of the last two radiological reaction types was radiation cirrhosis (postnecrotic cirrhosis).
Different radiological reaction types of radiation liver injury on multiphase CT have different pathological basis, and multiphase contrast-enhanced CT may help distinguish the radiation reactions from tumor recurrence.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 09/2007; 27(8):1231-5.
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ABSTRACT: To observe the changes of the cytokines following stereotactic irradiation for hepatocarcinoma with cirrhosis in rabbits.
Sixteen rabbits with liver cirrhosis and hepatocarcinoma (experimental group) were randomized into two equal groups to receive stereotactic irradiation at single dose of 20 and 30 Gy, respectively. Eight rabbits with hepatocarcinoma (control group) were divided into two equal groups and treated in identical manner. All the rabbits were killed 3 weeks after irradiation, and EV two-step method was used to observe the cytokine changes of proliferating cell nuclear antigen (PCNA) and glutathione S-transferase pi (GST-pi) after irradiation.
After irradiation, PCNA and GST-pi expression showed significant difference in the adjacent liver tissue between the experimental and control rabbits with irradiation at 20 Gy (P=0.010), but not with the irradiation dose of 30 Gy (P=1.000). Irradiation at different doses resulted in significant difference in the cytokine expression in the experimental rabbits (P=0.010). In the liver tissue exposed to irradiation, different irradiation doses resulted in significant difference in PCNA and GST-pi protein expression (P=0.010).
For hepatocarcinoma with cirrhosis in rabbits, radiation at the single dose of 30 Gy produces better response than 20 Gy, and PCNA and GST-pi may serve as good indexes for evaluating the therapeutic effect.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 03/2007; 27(3):279-82.
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ABSTRACT: To review the appearance of radiation-induced liver injury on computer tomography for quantitative assessment of dosimetric changes in different radiological reactions and the influence of time-effect.
The focal liver reactions of 35 patients treated with three-dimensional conformal radiation therapy (3-D CRT) for liver malignancies were evaluated, with the applied doses of 36-65 Gy in 4-28 fractions completed in 8-41 days. All patients received nonenhanced CT scan and arterial-dominant phase contrast-enhanced CT scan 1-6 months after therapy. The liver tissue density in irradiated and nonirradiated liver was compared, and the reaction type and the threshold dose determined radiologically.
On at least one follow-up examination, 51.4% of patients were found to have a focal radiation reaction in the liver. The radiation reaction was hypodense in 43.75% of the follow-up nonenhanced CT examinations and in 19.23% of arterial-dominant phase contrast-enhanced CT scans. It was hyperdense in 42.31% of arterial-dominant phase contrast-enhanced CT scans. The median threshold dose inducing a radiation reaction was 30.8 Gy (range 18-42.8 Gy). The detected threshold dose was positively correlated with the time of detection of the reaction (P=0.041), with a correlation coefficient of -0.473. On arterial-dominant phase contrast-enhanced CT scans, the threshold dose was significantly higher for hyperdense than for hypodense changes (P=0.017). In additional follow-up, the reaction volume decreased and the reaction types changed on arterial-dominant phase contrast-enhanced CT scans.
The threshold dose can be different in different radiological reaction types on multiphase CT scans. The detected threshold dose is inversely correlated with the time of detection of the early reaction. Multiphase contrast-enhanced CT is helpful to distinguish radiation reactions from recurrent tumors.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 02/2007; 27(1):109-12.
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ABSTRACT: To investigate the effects of pentoxifylline (PTX) on radiation induced-cell cycle redistribution and radiosensitivity of human hepatocellular carcinoma cell line Hep3b.
MTT assay was performed to evaluate the cytotoxicity of PTX on p53-defective human hepatocellular carcinoma cell line Hep3b and clonogenic assay employed to observe its effects on the radiosensitivity of the cells quantified by calculating the sensitive enhancement ratio (SER). Flow cytometry was performed to observe the cell cycle changes of Hep3b cells in response to X-ray irradiation and the interventional effect of PTX.
The cytotoxicity of PTX on the cells increased in a dose-dependent manner following a 48-hour treatment, with the optimal dose range of 1-5 mmol/L. A sub-toxic dose of PTX at 2 mmol/L was then used in subsequent experiments. Clonogenic survival assays up to 12 Gy demonstrated that p53-defective Hep3b cells (SER of 2.68+/-0.24) were sensitized by PTX (2 mmol/L). PTX (2 mmol/L) treatment following exposure to irradiation (6 Gy) resulted in abrogation of radiation-induced G(2)/M arrest of Hep3b cells, and the proportions of Hep3b cells in G(2)/M phase were 86.8% and 14.8% after exposure to 6 Gy alone and 6 Gy plus 2 mmol/L PTX, respectively.
Radiosensitization by PTX is possibly associated with the abrogation of G(2)/M arrest in Hep3b cells following radiation exposure, suggesting that potential clinical application of PTX may enhance the efficacy of radiotherapy against hepatocellular carcinoma.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 04/2006; 26(3):305-7.
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ABSTRACT: To explore the clinical value of CT-guided percutaneous needle biopsy (PCNB) for diagnosis of atypical pulmonary tuberculosis.
The data from 15 patients undergoing diagnostic PCNB for pulmonary tuberculosis were retrospectively analyzed. Prior to PCNB, definitive diagnosis failed to be obtained from other examinations including sputum smear in 10 cases, fiberoptic bronchoscopy in 12, chest X-ray in 13, CT scan in 15, and position emission tomography (PET) with CT examination in 1 case.
Sputum smears and fiberoptic bronchoscopy both failed to produce positive results and pulmonary and/or mediastinal lesions were shown by chest X-ray, CT, and PET-CT. Pulmonary tuberculosis were diagnosed in the 15 cases by histopathological examination following PCNB, and pneumothorax in relation to PCNB occurred in 3 cases and hemoptysis in 1 case.
PCNB provides a safe and accurate means for diagnosis of pulmonary tuberculosis when imaging and other diagnostic modalities fail to result.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 03/2006; 26(2):214-6.
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ABSTRACT: To explore the clinical value of (18)F-fluorodeoxyglucose positron emission tomography ((18)FDG-PET) in three-dimensional conformal radiotherapy (3D-CRT) for locally residual and recurrent nasopharyngeal carcinoma (NPC).
Between July 1998 and October 2000, 36 patients with recurrent or residual NPC confirmed by biopsy after initial radiotherapy underwent treatment with 3D-CRT. CT simulation was performed and (18)FDG-PET carried out for delineating the gross tumor volume (GTV) in all the patients. The radiotherapy was designed by 3D treatment planning system and dose-volume histogram (DVH) was used to verify and optimize the radiation plan. Acute and late radiation injuries, survival rates and GTV delineated by CT and (18)FDG-PET were observed in all the cases after the treatment.
In comparison with CT simulation, (18)FDG-PET delineated similar GTV in 36.1% (13/36) cases, larger and smaller GTV in 16.7% (6/36) and 47.2% (17/36) of the cases, respectively. After 3D-CRT, 97.2%, 75.0%, and 58.3% of the patients were free of evidence of 1-, 2-, and 3-year local failure, respectively, and the 1-, 2-, and 3-year survival rate of the patients were 94.4%, 86.1%, and 72.0%. The 1-, 2-, 3-year tumor-free survival rates were 85.7%, 76.6%, and 56.1% respectively. Acute xerostomia, nasopharyngitis and oropharyngitis were the most common complications in relation to the radiation, but all within acceptable range of grades 1 or 2. The lesions resulting from the radiation included hearing loss, trismus, dysphagia, chronic sinusitis, and cranial neuropathy, with the incidences of 5.6% (2/36), 25.0% (9/36), 8.3% (3/36), 33.3% (12/36), and 13.9% (5/36) respectively.
3D-CRT combined with (18)FDG-PET for delineation of GTV is effective and feasible in the treatment of locally residual or recurrent nasopharyngeal carcinoma after initial radiotherapy, producing good responses without serious complications.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 01/2006; 25(12):1568-70.
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ABSTRACT: To investigate the killing effect and radiosensitization of double suicide gene mediated by adenovirus on colorectal carcinoma cells.
Colorectal carcinoma cell line SW480 was transfected with adenovirus expression vector containing cytosine deaminase (CD) and thymidine kinase (TK) fusion gene. The expression of CD-TK fusion gene was detected by reverse transcriptase-polymerase chain reaction. The toxic effect of ganciclovir (GCV) and 5-fluorocytosine (5-FC) on infected cells was determined by MTT assay. The radiosensitization of double suicide gene was evaluated by clonogenic assay.
After prodrugs were used, the survival rate of colorectal carcinoma cells was markedly decreased. When GCV and 5-FC were used in combination, the cytotoxicity and bystander effect were markedly superior to a single prodrug (chi2 = 30.371, P<0.01). Both GCV and 5-FC could sensitize colorectal carcinoma cells to the toxic effect of radiation, and greater radiosensitization was achieved when both prodrug were used in combination.
CD-TK double suicide gene can kill and radiosensitize colorectal carcinoma cells.
World Journal of Gastroenterology 06/2005; 11(20):3051-5. · 2.47 Impact Factor
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ABSTRACT: To explore the expression of Tiam1 gene in colorectal carcinoma and its correlation with tumor metastasis.
Expressions of Tiam1 gene in 8 colorectal carcinoma cell lines were detected by reverse transcriptase-polymerase chain reaction. In vitro invasiveness was determined by means of Matrigel invasion assay. The correlation of Tiam1 expression with the invasive ability was also analyzed.
Tiam1 gene was highly expressed in LoVo and SW620, which were established from metastatic colorectal carcinomas in comparison with LS174T, SW480, HCT116, LST, HRT-18 and Hee8693, which were established from primary colorectal carcinomas. In vitro cell invasion demonstrated that LoVo and SW620 had a higher invasive ability than LS174T, SW480, HCT116, LST, HRT-18 and Hee8693. The expression of Tiam1 gene was highly related to the metastatic potential of colorectal carcinoma cells.
Tiam1 gene may play an important role in invasion and metastasis of colorectal carcinoma and is a metastasis-related gene.
World Journal of Gastroenterology 03/2005; 11(5):705-7. · 2.47 Impact Factor
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ABSTRACT: To evaluate the clinical therapeutic effect of three-dimensional conformal hypofractionated high-dose radiotherapy combined with chemotherapy for non-small-cell lung cancer (NSCLC).
The combined treatment was carried out in 63 cases of NSCLC and the short-term effects, the patients' survival rate and complications were observed.
Local control of tumor growth was achieved in 79.1% of the patients and the survival rates of 1, 3, and 5 years were 65.2%, 23.2%, and 10.4%, respectively. The median actual survival of the patients was 14 months. Late radiation pneumonitis (grade 3 or 4) and oesophagitis occurred in 9.5% and 4.8% of the patients, respectively.
Three-dimensional conformal hypofractionated high-dose radiotherapy combined with chemotherapy can be an effective and practical approach to treat NSCLC, but local recurrence remains the major factor of treatment failure.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 12/2004; 24(11):1297-8.
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ABSTRACT: To evaluate the effects of 3-dimensional conformal radiation therapy combined with chemotherapy for rectal cancer of postoperative local recurrence.
Fifty-six rectal cancer patients of postoperative local recurrence were treated with 3-dimensional conformal radiotherapy (66 approximately 68 Gy in 2 Gy daily fraction) combined with chemotherapy (5-Fu 0.75, Days 1 - 5 and Days 29 - 33; Cisplatin 40 mg, Days 1 - 3 and Days 29 - 31). Acute and late radioreactions and survival rates were observed. Kaplan-Meier method was used for survival analysis.
The 1, 2, 3 years local control rate was 87.5%, 66.1%, 38.2%, respectively. The 1, 2, 3 years disease-free survival and overall were 82.7%, 56.6%, 30.1% and 88.3%, 66.8%, 44.2%, respectively. The median survival was 25.3 months. Acute small enteritis were the most common but acceptable radioreactions Grade 1 approximately 2. No late radioreactions occurred.
3-dimensional conformal radiotherapy combined with chemotherapy can be considered as an effective and feasible approach to cancer patients of postoperative local recurrence.
Zhonghua wai ke za zhi [Chinese journal of surgery] 09/2004; 42(15):901-3.
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ABSTRACT: To evaluate the therapeutic efficacy of three-dimensional conformal radiotherapy (3D-CRT) combined with transcatheter arterial chemoembolization (TACE) on the patients with hepatocellular carcinoma (HCC).
Between 1998 and 2001, 94 patients with HCC received 3D-CRT combined with TACE. A total 63 patients had a Okuda stage I lesion and 31 patients had stage II. The median tumor size was 10.7 cm (range 3.0-18 cm), and liver cirrhosis was present in all the patients. There were 43 cases of class A and 51 class B. TACE was performed using lipiodol, 5-fluorouracil, cisplatin, doxorubicin hydrochloride and mitomycin, followed by gelatin sponge cubes. Fifty-nine patients received TACE only one time, while the others 2 to 3 times. 3D-CRT was started 3-4 wk after TACE. All patients were irradiated with a stereotactic body frame and received 4-8 Gy single high-dose radiation for 8-12 times at the isocenter during a period of 17-26 d (median 22 d).
The median follow-up was 37 mo (range 10-48 mo) after diagnosis. The response rate was 90.5%. The overall survival rate at 1-, 2-, and 3- year was 93.6%, 53.8% and 26.0% respectively, with the median survival of 25 mo. On univariate analysis, age (P=0.026), Child-Pugh classification for cirrhosis of liver (P=0.010), Okuda stage (P=0.026), tumor size (P=0.000), tumor type (P=0.029), albuminemia (P=0.035), and radiation dose (P=0.000) proved to be significant factors for survival. On multivariate analysis, age (P=0.024), radiation dose (P=0.001), and tumor size (P=0.000) were the significant factors.
3D-CRT combined with TACE is an effective and feasible approach for HCC. Age, radiation dose and tumor size were found to be significant prognostic factors for survival of patients with HCC treated by 3D-CRT combined with TACE. Further study for HCC is needed to improve the treatment efficacy.
World Journal of Gastroenterology 09/2004; 10(15):2184-9. · 2.47 Impact Factor
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ABSTRACT: To investigate KAI1 gene expression in the progression of human colonic carcinoma and its clinical significances.
KAI1 expression was detected by in situ hybridization and immunohistochemistry in the 4 established cell lines of colorectal carcinoma with different metastatic potentials, and in 80 specimens of colonic carcinoma, 21 colonic carcinoma specimens with lymphatic metastasis and 20 controls of normal colonic mucosa.
The expressions of KAI1 in HT29 and SW480 cell lines were higher than those in LoVo and SW620. The expression of KAI1 gene was significantly higher in colorectal carcinoma compared with normal colonic mucosa and lymphatic metastasis (chi(2)=46.838, P<0.01). The expression of KAI1 gene had no relationship with histological grade. The KAI1 expressions in Dukes A and B carcinoma were higher at both mRNA and protein levels compared to Dukes C carcinoma (chi(2)=16.061, P<0.05). The expression of KAI1 in colonic carcinoma specimens with lymphatic metastasis was almost lost. The results of in situ hybridization were in concordance with immunohistochemistry.
KAI1 is highly related to the metastasis of colonic carcinoma and may be a useful indicator of metastasis in colonic carcinoma.
World Journal of Gastroenterology 09/2004; 10(15):2245-9. · 2.47 Impact Factor
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ABSTRACT: Hepatocellular carcinoma (HCC) often results in portal vein tumor thrombus (PVTT), and the prognosis of the patients is extremely poor. It has been reported that some oversea scholars achieved fine therapeutic effects in treatment of this disease by 3-dimensional conformal radiation therapy (3DCRT) in fractionated conventional dose. The aim of this study was to evaluate the efficacy of 3-dimensional conformal hypofractionated single high-dose radiotherapy combined with transcatheter arterial chemoembolization (TACE) for portal vein tumor thrombus in patients with hepatocellular carcinoma.
From May 1998 to December 2000, 35 patients with unresectable HCC complicated with PVTT were treated with 3-dimensional conformal hypofractionated single high-dose radiotherapy and TACE. According to the volume of the tumors, radiotherapy was performed at an exposure of 4-8 Gy/time, 3 times/week, 48-60 Gy, 8-12 times, 3.0-3.5 weeks. The objective responses were analyzed and the survival rates were assessed from the date of the beginning of treatment using the Kaplan-Meier method. The Cox proportional hazards model was used to analyze the prognostic factors.
The objective response was 71.4%. The overall survival rates were 59.3%, 31.6%, and 26.6% at 1, 2, 3 years, respectively, with a median survival time of 11 months. Cox proportional hazards model analysis showed that Child-Pugh class was the most important prognostic factors for the survival probability of the patients. Radiation-induced liver disease (RILD) and gastrointestinal bleeding were the most common treatment-related complications.
3-Dimensional conformal hypofractionated single high-dose radiotherapy combined with TACE is an effective and feasible approach to treat PVTT in unresectabe HCC patients. Child-Pugh class was identified as a predictor for PVTT in unresectabe HCC patients.
Ai zheng = Aizheng = Chinese journal of cancer 07/2004; 23(7):825-8.
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ABSTRACT: To investigate the radiosensitization by pentoxifylline (PTX) in human hepatoma cell line HepG2 and its possible mechanism.
MTT assay was used to evaluate the cytotoxicity of PTX and clonogenic assay employed to observe its effects on the radiosensitivity of the cells quantified by calculating the sensitive enhancement ratio (SER). Flow cytometry was performed to observe the changes in the cell cycle of HepG2 cells in response to X-ray irradiation and the effect of PTX intervention.
The cytotoxicity of PTX increased in a dose-dependent manner following a 24-hour treatment, with the optimal dose range of 1-5 mmol/L. A sub-toxic dose of PTX at 2 mmol/L was used in the subsequent experiments. PTX significantly reduced the clonogenic activity and enhanced the radiosensitivity of HepG2 cells with a SER of 1.3+/-0.16 at the dose of 2 mmol/L. Irradiation resulted in cell cycle arrest at G2 phase in HepG2 cells, and the percentages of HepG2 cells in G2-M phase were 32.15% and 19.52% respectively after exposure to 6 Gy radiation alone and to 6 Gy plus 2 mmol/L PTX at 20 h, demonstrating the effectiveness of PTX in resolving radiation-induced G2 arrest.
Radiosensitization by PTX is possibly associated with the abrogation of G2 arrest in HepG2 cells following radiation exposure.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 05/2004; 24(4):382-5.
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ABSTRACT: To evaluate the therapeutic effects of three-dimensional conformal radiotherapy (3D-CRT) for hilar cholangiocarcinoma.
Twenty-one patients with unresectable hilar cholangiocarcinoma were treated with 3D-CRT and the acute effects and survival rates were observed.
Eleven (52.38%) of the 21 patients achieved a complete or partial remission. One, 2- and 3-year overall survival rates were 42.85% (9/21), 28.57% (6/21), and 14.29% (3/21), respectively, with a median overall survival rate of 16.5 months. Eight patients developed duodenal ulcer as a late complication of radiotherapy, and 4 patients died of gastrointestinal bleeding.
3D-CRT can be considered as a valuable palliative approach for unresectabe hilar cholangiocarcinoma, and effective management of the late complications of radiotherapy play a key role in increasing survival rates of the patients.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 12/2003; 23(11):1217-8.
