[Show abstract][Hide abstract] ABSTRACT: To diversify usage of aquacultural red sea cucumber, new Kimchi containing red sea cucumber was developed and its quality and consumer preference were studied. The pH and total acidity, index of Kimchi ripening, decreased till 7 days of storage, then pH change became blunt and finally reached optimum pH 4-4.5. The amount of reducing sugar gradually decreased during storage period. The number of lactic acid bacteria increased in early fermentation period in both Kimchi containing boiled and fresh red sea cucumber, however, more lactic acid bacteria found in fresh red sea cucumber containing Kimchi. Preference of Kimchi containing boiled or fresh red sea cucumber compared to control Kimchi containing fermented salted fish was evaluated. The results revealed there were no differences in color, flavor, texture, taste, after taste and overall preference.
Journal of Applied Biological Chemistry 06/2012; 55(2). DOI:10.3839/jabc.2011.071
[Show abstract][Hide abstract] ABSTRACT: Artemisia capillaris is a perennial herb that belongs to the family Campositae spp, and has been utilized in Korea for the treatment of abdominal pain, hepatitis, chronic liver disease, and coughing. The anti-inflammatory activity of the ethanol extract of A. capillaris (EtEAC) was investigated in this study. EtEAC did not affect the viability of various cells in a concentration range of 0-200 μg/mL. The DPPH radical scavenging ability of 10 μg/mL of EtEAC was comparable to that of 10 μg/mL of catechin. EtEAC exerted an anti-inflammatory effect on the mRNA expression levels of typical inflammatory cytokines (IL-6, IL1β) and cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Additionally, EtEAC reduced nitric oxide (NO) production via the downregulation of inducible nitric oxide synthase transcription, rather than by scavenging NO. EtEAC exerted its anti-inflammatory effects by suppressing the activation of p38 and ERK at 200 μg/mL in LPS-stimulated RAW 264.7 cells.
Journal of the Korean Society for Applied Biological Chemistry 01/2010; 53(3). DOI:10.3839/jksabc.2010.043 · 0.69 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The anti-fibrotic effects of hot water extract of adventitious root culture of Panax ginseng (ARCP) and the possible mechanisms were investigated on hepatotoxicity model mice. Fibrosis was induced by a mild treatment of . Then silymarin as a positive control drug and ARCP or carrier alone as a negative control were treated. Serum GPT, GOT and ALP activity levels were lowered by 25, 21 and 11% for silymarin treated group and by 48, 39 and 14% for ARCP treated group compared to carrier treated alone. Hepatic collagen for ARCP treatment group was reduced by 18% and MDA contents decreased a little more. Pro-fibrotic gene (, TIMP-1 and ) expression increased following the treatment, but both the silymarin and the ARCP treatments decreased the expressions of these genes by 20% to 50%. The antioxidant effect of ARCP was studied by DPPH free radical scavenging activity. In addition, a generation of reactive oxygen species (ROS) was also reduced in HepG2 cells upon the ARCP treatment. In summary, ARCP has antioxidant property, and can have some protection against oxidative stress; more importantly, ARCP can efficiently protect mice against fibrosis.
Journal of Applied Biological Chemistry 01/2007; 50(2).
[Show abstract][Hide abstract] ABSTRACT: The characteristics of a lectin from the marine bivalve Ruditapes philippinarum (Manila clam) were investigated in this study. A method was developed for the isolation of the Manila clam lectin (MCL). Affinity chromatography using mucin-Sepharose, ion-exchange chromatography with DEAE-Toyoperl, and gel filtration with Superose 6 were used for MCL isolation. SDS-PAGE showed that the MCL protein had a molecular mass of 138 kDa, and consisted of 74-, 34-, and 30-kDa subunits. The native lectin in solution behaved as a 274-kDa protein in gel filtration chromatography. The lectin activity of MCL was Ca2+ -dependent, and the optimal Ca2+ concentration for MCL activity was 20 mM. MCL activity was stable between pH 6 and pH 9, and was temperature-dependent; incubation of MCL at 90 degrees C led to irreversible denaturation. The activity of MCL was not inhibited by the presence of monosaccharides, such as Man, Fuc, Gal, Glc, GlcNAc, and NeuNAc. In contrast, the lectin activity of MCL was strongly inhibited by the presence of porcine mucins. MCL activity was also inhibited by N-acetyl-d-galactosamine, human embryonic alpha-1-acid glycoprotein, and highly branched mannans from marine halophilic bacteria. It appears that MCLs have unusual carbohydrate specificities for N-acetyl-d-galactosamine, which contains both mucin-type carbohydrate chains and highly branched mannans. Immunofluorescence staining revealed that MCL was bound to the surfaces of purified hypnospores from Perkinsus sp., which is a protozoan parasite of Manila clams.
[Show abstract][Hide abstract] ABSTRACT: The cytotoxic activity of oxysterols, 7 beta-hydroxycholesterol (7 beta-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G(2)/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7 beta-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G(2)/M phase.
Life Sciences 03/2003; 72(12):1389-99. DOI:10.1016/S0024-3205(02)02377-9 · 2.70 Impact Factor