Masanori Shinzato

Fujita Health University, Nagoya, Aichi, Japan

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Publications (45)57.29 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The efficacy of skin regeneration devices consisting of nonwoven filters and peripheral blood cells was investigated for wound healing. We previously found that human peripheral blood cells enhanced their production of growth factors, such as transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor, when they were captured on nonwoven filters. Cells on biodegradable filters were expected to serve as a local supply of growth factors and cell sources when they were placed in wounded skin. Nonwoven filters made of biodegradable polylactic acid (PLA) were cut out as 13-mm disks and placed into cell-capturing devices. Mouse peripheral blood was filtered, resulting in PLA filters with mouse peripheral blood cells (m-PBCs) at capture rates of 65.8 ± 5.2 %. Then, the filters were attached to full-thickness surgical wounds in a diabetic db/db mouse skin for 14 days as a model of severe chronic wounds. The wound area treated with PLA nonwoven filters with m-PBCs (PLA/B+) was reduced to 8.5 ± 12.2 % when compared with day 0, although the non-treated control wounds showed reduction only to 60.6 ± 27.8 %. However, the PLA filters without m-PBCs increased the wound area to 162.9 ± 118.7 %. By histopathological study, the PLA/B+ groups more effectively accelerated formation of epithelium. The m-PBCs captured on the PLA filters enhanced keratinocyte growth factor (FGF-7) and TGF-β1 productions in vitro, which may be related to wound healing. This device is useful for regeneration of wounded skin and may be adaptable for another application.
    Journal of Artificial Organs 05/2015; DOI:10.1007/s10047-015-0845-x · 1.44 Impact Factor
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    ABSTRACT: Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2-22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2-1.8 μm captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.
    Journal of Artificial Organs 10/2014; 18(1). DOI:10.1007/s10047-014-0794-9 · 1.44 Impact Factor
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    ABSTRACT: Aloe-emodin (AE), a natural anthraquinone compound, has been reported to exhibit anticancer activity in various cancer cell lines and anti-inflammatory effects in murine macrophages. In the present study, we investigated the cancer chemopreventive effects of AE in an Apc-deficient Min mouse model. In the first experiment, male Min mice were fed a basal diet or diets containing 5 ppm AE and 10 ppm AE for 12 weeks. The dietary administration of 5 ppm AE significantly reduced the number of colorectal tumors. In a second experiment, we investigated the effects of AE on colitis-related colon carcinogenesis in Min mouse treated with dextran sodium sulfate (DSS). Female Min mice were administered 1% DSS in their drinking water for 7 days. AE was given to mice in their diet at a dose of 5 or 50 ppm for 5 weeks. Feeding with AE significantly reduced the number of colorectal tumors. When proliferation of cells in normal-appearing colonic mucosa was assessed by monoclonal anti-rat Ki-67 antibody (MIB-5) immunohistochemistry in experiments 1 and 2, the AE treatment significantly decreased the mean MIB-5-labeling index. These results suggest that the dietary administration of low-dose AE may have chemopreventive effects against development of colorectal tumors in Min mice, possibly in part by reducing cell proliferation in colorectal mucosa.
    Asian Pacific journal of cancer prevention: APJCP 07/2014; 15(14):5587-92. DOI:10.7314/APJCP.2014.15.14.5587 · 2.51 Impact Factor
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    ABSTRACT: After finding tonsil-like structures near the entrance of vagina of a laboratory shrew (Suncus murinus), which we subsequently designated as vaginal tonsils, we performed detailed immunohistochemical and developmental studies. The location of T and B cells in the vaginal tonsils differed from that in the palatine tonsils or that in the lymphoid nodes of other animals. The boundary between the germinal center region and the region encompassing follicular interfollicular tissue was not clear. B cells were widely distributed and very dense in the parenchyma, but they were scattered in the epithelial area (B cells were present in around 90% of the vaginal tonsil tissue). In contrast, T cells were scattered in the parenchyma and in the epithelial area (T cells were present in around 10% of the vaginal tonsil tissue). B cells were more prominent than T cells throughout the development of these structures and the epithelium was invaded by many immigrating cells. The size of the vaginal tonsils changed during postnatal development. Vaginal tonsils are structurally similar to other tonsils, and they may function to protect the vagina from infection.
    Okajimas Folia Anatomica Japonica 02/2013; 89(4):105-12. DOI:10.2535/ofaj.89.105
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    ABSTRACT: High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and O6-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)- induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2% or 5% HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2% HTPG group (but not in the 5% HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2% HTPG (but again not 5% HTPG) than in controls. These results in dicate that HTPG, at 2% in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.
    Asian Pacific journal of cancer prevention: APJCP 05/2012; 13(5):1983-8. DOI:10.7314/APJCP.2012.13.5.1983 · 2.51 Impact Factor
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    ABSTRACT: We investigated the distribution of T lymphocytes, B lymphocytes, and S-100 protein-immunoreactive dendritic-like in the anal tonsil of the laboratory shrew, Suncus murinus. In adult animals, T lymphocytes were located mainly at the periphery of the anal tonsil, especially around small blood vessels. B lymphocytes were located in the central and subepithelial region of the anal tonsil, which includes primary lymphoid follicles, and in which there are small numbers of scattered T lymphocytes. B and T lymphocytes were distributed over 72.7 and 27.3% of the tonsillar area, respectively. However, their areas of distribution were not clearly distinguished. The areas containing B lymphocytes were enriched in S-100 protein antibody-immunoreactive cells, which exhibited a dendritic shape. These S-100-positive cells appeared to be identical to the follicular dendritic cells (FDC) seen in the follicles of lymphoid organs. These results suggest that the anal tonsils constitute one of the gut-associated lymphoid tissues (GALT), and that a function of the anal tonsil includes the capture of intruding antigens that would generate protective antibody responses. Microsc. Res. Tech., 2011. © 2010 Wiley-Liss, Inc.
    Microscopy Research and Technique 01/2010; 74(9):819-24. DOI:10.1002/jemt.20961 · 1.15 Impact Factor
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    ABSTRACT: We studied the role of osteopontin (OPN) in host responses against rotavirus (RV) infection. OPN knockout (OPN-KO) suckling mice were more susceptible to RV (strain EW) infection and showed prolonged diarrhea duration compared to wild-type (WT) suckling mice. OPN in the small intestine of WT mice was expressed after 48 h post-infection. On day 2 postinfection, mRNA levels of interleukin-1beta, tumor necrosis factor-alpha, and interleukin-15 in OPN-KO mice were lower than in WT mice, although mRNA expression of Th-1- and Th-2-related cytokines in the small intestine were nearly the same between OPN-KO and WT mice. These results suggested that OPN is involved in innate responses against RV infection.
    Viral immunology 05/2009; 22(2):139-44. DOI:10.1089/vim.2008.0054 · 1.45 Impact Factor
  • K. Shimpo · T. Chihara · M. Shinzato · H. Beppu · T. Kaneko · M. Shamoto · H. Kuzuya
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    ABSTRACT: We examined the modifying effects of freeze-dried whole-leaf Aloe arborescens Miller var. natalensis Berger (Japanese name, Kidachi aloe; designated as ‘ALOE’) on 1,2-dimethylhydrazine (DMH)-induced colorectal tumorigenesis in mice. Female ICR mice (7-weeks old) were given a basal diet or a diet containing 1, 0.5 or 0.1% ALOE for 32 weeks. One week later, all mice were injected i.p. with DMH (20 mg/kg, once weekly for 10 weeks) or vehicle (1 mM EDTA solution, pH 6.5). At 32 weeks, animals were killed by exsanguination, and the colorectums were processed for histological examination. The administration of ALOE (1, 0.5 or 0.1% in diet) did not induce diarrhea or reduction of body weight. In mice given DMH and 1% ALOE (Group 2), the incidence and multiplicity of colorectal adenomatous hyperplasias were significantly decreased as compared with mice given DMH alone (Group 1) (both p < 0.05), whereas the incidence and multiplicity of tumors (adenoma and adenocarcinoma) in Group 2 tended to be lower than those in Group 1. In addition, the incidence and multiplicity of the colorectal proliferative lesions (the total of adenomatous hyperplasias, adenomas and adenocarcinomas in mouse colorectum) in Group 2 were significantly decreased as compared with Group 1 (both p < 0.01). No colorectal proliferative lesions were found in animals that did not receive DMH. These results indicated that ALOE reduces the incidence and multiplicity of DMH-induced colorectal proliferative lesions, especially adenomatous hyperplasia, in mice.
    Pharmaceutical Biology 10/2008; 41(8):631-636. DOI:10.1080/13880200390502612 · 1.24 Impact Factor
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    ABSTRACT: A 73-year-old man who had been undergoing chronic haemodialysis (CHD) for 3 years developed haemophagocytic syndrome (HPS) that might have been triggered by Epstein-Barr virus (EBV) infection. the patient finally died of miliary tuberculosis (TB) reactivation that promoted the progression of HPS. Immunological abnormalities in patients undergoing CHD may be notable. the early diagnosis of TB reactivation may be important for reducing the mortality in cases of HPS, as a high incidence of TB is encountered in patients undergoing CHD. In contrast, the simultaneous occurrence of an EBER-positive hybridization signal with T cell-specific immunolabelling of CD45RO cells was well detected in the spleen and lymph nodes, and interferon gamma was elevated in the serum. These findings coincide with the reported preferential expansion of T cells rather than B cells in EBV infection, and support the hypothesis that systemic hypercytokinaemia caused by the proliferation of EBV-infected T cells may play a crucial role in the development of HPS.
    Nephrology 06/2008; 7(2):61 - 65. DOI:10.1046/j.1440-1797.2002.00078.x · 2.08 Impact Factor
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    ABSTRACT: It is well known that avian yolk sac is involved in both primitive and definitive erythropoiesis during embryonic development. Definitive erythropoiesis is first detected at about 4-5 days incubation and its maximum activity is reached between day 10 and 15 of incubation, ending between days 18 and 20 of incubation. We confirmed the definitive erythropoietic foci in the chicken yolk sac throughout the 5th to 19th day of incubation by histochemical light and electron microscopy. The definitive erythropoietic foci were observed in the yolk sac endodermal layer from day 5 until day 19, just before hatching. Ultrastructurally, definitive erythropoietic foci were observed extravascularly in the yolk sac endodermal cell layer in direct contact with the vitellolysis zone. These findings provide a basis for clarifying definitive erythropoiesis in vertebrates.
    Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia 01/2008; 113(1):9-16.
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    ABSTRACT: Light and electron microscopic examination of first-trimester and term human placental tissues were performed to identify erythrocytes containing hemoglobin in the villous trophoblast cell layer. Erythrocytes were not identified in chorionic villous epithelium at week 7 of gestation. These cells first appeared in the villous cytotrophoblast at week 8, and continued to be present in the villous cytotrophoblast until week 9, as shown by benzidine staining. At week 12 gestation, a cluster of erythrocytes was present in a villous syncytial sprout. At 40 and 41 weeks gestation, erythrocytes were located in the villous cytotrophoblast cell layer. Electron microscopic observations focused on the cytoplasm of villous cytotrophoblast at week 8, the syncytial sprout at week 12 and the cytotrophoblast cell layer at term, confirmed the presence of erythrocytes at an extravascular location, as observed by light microscopy.
    Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia 07/2007; 112(3):191-8.
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    ABSTRACT: Osteopontin (OPN) knockout mice (OPN-KO mice) died of Plasmodium chabaudi chabaudi infection, although wild-type (WT) mice had self-limiting infections. OPN was detected in the WT mice at 2 days postinfection. OPN-KO mice produced significantly smaller amounts of interleukin-12 and gamma interferon than WT mice produced. These results suggested that OPN is involved in Th1-mediated immunity against malaria infection.
    Infection and Immunity 05/2006; 74(4):2423-7. DOI:10.1128/IAI.74.4.2423-2427.2006 · 3.73 Impact Factor
  • R. Teradaira · M. Shinzato · H. Beppu · K. Fujita
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    ABSTRACT: The antigastric effects of Aloe arborescens Miller var. natalensis Berger extract with high molecular weight components ((A) MW over 5000 and (B) MW over 50 000)) were examined by changes of ulcer index with three different experimental models in rats. The suppressive effects were evaluated under (1) stress (immobilization in water) and (2) ligation of pylorus, and the healing effects were likewise examined with the (3) acetic acid test. Both fractions showed a slight suppressive effect on the stressed animals. However, in the groups with pylorus ligation, Aloe extract (A) significantly disclosed suppressive effects. Furthermore, the same Aloe fraction heated at 100°C for 15 min also demonstrated a significant effect on animals with pylorus ligation. Rats induced with acetic acid exhibited a significant healing effect. However, fraction (B) produced only a slight beneficial effect. These results suggest the existence of both suppressive and healing substances on gastric ulcer in the Aloe extract within the range of MW 5000–50 000. These effects were confirmed by micro- and macroscopic findings.
    Phytotherapy Research 01/2006; 7(7):S34 - S36. DOI:10.1002/ptr.2650070712 · 2.66 Impact Factor
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    ABSTRACT: We examined the modifying effect of freeze-dried whole-leaf Aloe arborescens Miller var. natalensis Berger (Kidachi aloe in Japan; designated as 'ALOE') on azoxymethane (AOM)-induced intestinal carcinogenesis in rats. Male F344 rats (4 weeks old) were fed basal diet or experimental diet containing 0.2% or 1% ALOE for 28 weeks. Starting two weeks later, the animals received subcutaneous injections of AOM once weekly for 10 weeks. The incidence of colorectal adenocarcinomas in the 0.2% (but not 1%) ALOE group showed a strong tendency for decrease (p = 0.056) from the control group. Further, the adenocarcinoma incidence in the entire intestine (small and large intestines) in the 0.2% ALOE group was significantly (p = 0.024) decreased compared to the control value. However, there were no significant differences in tumor multiplicities of colorectal or entire intestines among the 3 groups. In addition, we also studied the safety of long-term ingestion of ALOE as a health food or natural thickening stabilizer. Rats were fed the basal diet or 1% ALOE diet for 35 weeks without AOM treatment. Feeding with 1% ALOE did not affect most hematological and serum biochemical parameters in the rats. These results indicate that a low level of ALOE ingestion might have a mild suppressive effect on intestinal tumor growth without harmful side effects.
    Asian Pacific journal of cancer prevention: APJCP 01/2006; 7(4):585-90. · 2.51 Impact Factor
  • International Journal of Dermatology 07/2004; 43(6):451-3. DOI:10.1111/j.1365-4632.2004.02116.x · 1.31 Impact Factor
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    ABSTRACT: We observed nishikinezumi, cinnamon-coloured (NC)/Fujita (F) mice aged between 5 and 28 weeks. These NC mice have skin eruptions that resemble human atopic dermatitis (AD) under conventional circumstances. We investigated the skin of eruptive and non-eruptive lesions in NC/F mice by using haematoxylin-eosin (H&E) staining, toluidine blue staining and immunohistopathological study with immunoglobulin (Ig)EepsilonRI, CD23, interleukin (IL)-4, IL-5, interferon (INF)-gamma and Ia antigen. Histological examination of the eruptive lesions revealed the perivascular infiltration of many lymphocytes and mast cells into the upper dermis. Intracellular oedema of the epidermis, lymphocyte infiltration into the epidermis and liquefaction degeneration of the basal layer were also observed. The numbers of IL-4 and IL-5 positive cells in the eruptive lesions were larger than those of the non-eruptive lesions. IL-4 and IL-5 positive cells in the eruptive lesions increased weekly. Some IFN-gamma positive cells were observed in the eruptive lesions after 21 weeks. IFN-gamma positive cells were scarce in the skin of both the non-eruptive and eruptive lesions before 21 weeks. Serum IgE increased from 7 weeks to 21 weeks. We confirmed that these findings indicated that T helper (Th)2-dominant immunological activation transformed to a Th1-dominant situation. Many IgEepsilonRI positive cells were recognized in the dermis of the eruptive lesions by the time IgE had decreased. We assumed that the dermatitis before 21 weeks was an IgE-mediated allergy. We have previously reported that older NC/F mice had positive patch-test reactions to mites. Because serum IgE decreased after 21 weeks, dermatitis after 21 weeks might be associated more with cell-mediated delayed hypersensitivity than with IgE-mediated immediate allergy.
    Journal of the European Academy of Dermatology and Venereology 04/2004; 18(2):148-52. DOI:10.1111/j.1468-3083.2004.00855.x · 2.83 Impact Factor
  • Dermatology 02/2004; 208(2):183-4. DOI:10.1159/000076504 · 1.57 Impact Factor
  • Takamasa Yanagida · Mikihiro Shamoto · Akiko Osada · Masanori Shinzato
    Journal of Clinical and Experimental Hematopathology 01/2004; 44(1):11-16. DOI:10.3960/jslrt.44.11
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    ABSTRACT: Approximately 30 cases of carcinoid tumor of the kidney have been reported in the English literature, including three cases found as components of teratomas. Renal composite tumors associated with somatostatinoma have not been described. A 53-year-old female presented with an incidentally found right renal cystic lesion. Computed tomography demonstrated a cystic lesion associated with a solid nodule in the right kidney and postcontrast dynamic MRI revealed enhancement of the solid nodule. The patient underwent radical nephrectomy for the kidney lesion and is now well without recurrence 21 months after the operation. From the histopathological findings we diagnosed the cystic lesion as a composite tumor composed of mucinous cystadenoma and carcinoid tumor. Immunohistochemistry demonstrated the majority of cells of in carcinoid portion to be positive for antisomatostatin staining. The present case is the first documented composite tumor of mucinous cystadenoma and somatostatinoma of the kidney.
    International Journal of Urology 12/2003; 10(11):603-6. DOI:10.1046/j.1442-2042.2003.00698.x · 2.41 Impact Factor
  • Gen Niimi · Nobuteru Usuda · Masanori Shinzato · Yoichi Nagamura
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    ABSTRACT: The mouse visceral yolk sac (VYS) is widely known to play an important role as erythropoietic tissue during embryonic periods. Mouse VYS from embryonic days 9 to 12 was examined by light microscopy, electron microscopy and histochemical analysis with benzidine to detect the presence of hemoglobin with special reference to the development of VYS, the disappearance of the blood islands in VYS, and the appearance of a novel structure in the form of erythrocyte-like globules in VYS endodermal cells. The villous appearance of VYS became complicated by the development of VYS endodermal cells. The blood islands positive for the benzidine reaction were light microscopically detected on embryonic days 9, 10, and 11. They disappeared on embryonic day 12, however. Erythrocyte-like globules positive for the benzidine reaction were not observed in VYS endodermal cells on embryonic days 9, 10, and 11, but then appeared on embryonic day 12, by light and electron microscopy. Erythrocyte-like globules in VYS endodermal cells, which appear after the disappearance of blood islands in VYS, may participate in erythropoiesis during embryonic development.
    Annals of Anatomy - Anatomischer Anzeiger 07/2003; 185(3):201-5. DOI:10.1016/S0940-9602(03)80022-5 · 1.48 Impact Factor

Publication Stats

195 Citations
57.29 Total Impact Points


  • 1992–2014
    • Fujita Health University
      • • Faculty of Medical Management and Information Science
      • • Department of Pathology
      • • School of Medicine
      • • Institute for Comprehensive Medical Science (ICMS)
      Nagoya, Aichi, Japan
  • 2006
    • Hokkaido University
      • Institute for Genetic Medicine
      Sapporo, Hokkaidō, Japan