Madhoolika Agrawal

Banaras Hindu University, Benares, Uttar Pradesh, India

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Publications (39)63.35 Total impact

  • Article: Assessment of six Indian cultivars of mung bean against ozone by using foliar injury index and changes in carbon assimilation, gas exchange, chlorophyll fluorescence and photosynthetic pigments.
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    ABSTRACT: Six Indian cultivars of Vigna radiata L. (HUM-1, HUM-2, HUM-6, HUM-23, HUM-24 and HUM-26) were exposed with ambient and elevated (ambient + 10 ppb ozone (O3) for 6 h day-1) level of O3 in open top chambers. Ozone sensitivity was assessed by recording the magnitude of foliar visible injury and changes in various physiological parameters. All the six cultivars showed visible foliar symptoms due to O3, ranging 7.4 to 55.7 % injured leaf area. O3 significantly depressed total chlorophyll, photosynthetic rate (Ps), quantum yield (F v/F m) and total biomass although the extent of variation was cultivar specific. Cultivar HUM-1 showed maximum reduction in Ps and stomatal conductance. The fluorescence parameters also indicated maximum damage to PSII reaction centres of HUM-1. Injury percentage, chlorophyll loss, Ps, F v/F m and total biomass reduced least in HUM-23 depicting highest O3 resistance (R%).
    Environmental Monitoring and Assessment 03/2013; · 1.40 Impact Factor
  • Article: Impact of elevated CO(2) and elevated O(3) on Beta vulgaris L.: Pigments, metabolites, antioxidants, growth and yield.
    Sumita Kumari, Madhoolika Agrawal, Supriya Tiwari
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    ABSTRACT: The present study was conducted to assess morphological, biochemical and yield responses of palak (Beta vulgaris L. cv Allgreen) to ambient and elevated levels of CO(2) and O(3), alone and in combination. As compared to the plants grown in charcoal filtered air (ACO(2)), growth and yield of the plants increased under elevated CO(2) (ECO(2)) and decreased under combination of ECO(2) with elevated O(3) (ECO(2) + EO(3)), ambient O(3) (ACO(2) + AO(3)) and elevated O(3) (EO(3)). Lipid peroxidation, ascorbic acid, catalase and glutathione reductase activities enhanced under all treatments and were highest in EO(3.) Foliar starch and organic carbon contents increased under ECO(2) and ECO(2) + EO(3) and reduced under EO(3) and ACO(2) + AO(3.) Foliar N content declined in all treatments compared to ACO(2) resulting in alteration of C/N ratio. This study concludes that ambient level of CO(2) is not enough to counteract O(3) impact, but elevated CO(2) has potential to counteract the negative effects of future O(3) level.
    Environmental pollution (Barking, Essex: 1987) 01/2013; 174C:279-288. · 3.43 Impact Factor
  • Article: Differences in Ozone Sensitivity at Different NPK Levels of Three Tropical Varieties of Mustard (Brassica campestris L.): Photosynthetic Pigments, Metabolites, and Antioxidants
    Poonam Singh, Madhoolika Agrawal, Shashi Bhushan Agrawal
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    ABSTRACT: The effects of ambient O3 at two different levels of nitrogen, phosphorus, and potassium, (recommended and 1.5 times the recommended NPK) on three tropical varieties of mustard (Brassica campestris L. var. Kranti, Aashirwad and Vardan) were explored to unravel the mechanism of protection under higher NPK level at a rural experimental site using open top chambers. Ambient O3 concentrations ranged from 27.7 to 59.04ppb. Lipid peroxidation, antioxidative enzymes, and metabolites were higher, whereas photosynthetic pigments and protein were lower in all the varieties of mustard grown in non-filtered chambers than in filtered chambers. The magnitude of response varied with varieties, NPK levels and ages. Vardan showed a maximum stimulation in the antioxidative defense system, thus efficient scavenging of ROS produced by O3 and consequently conferred greater tolerance in terms of least reductions in pigments and protein as compared to Kranti and Aashirwad. The antioxidant defense system was not stimulated in response to 1.5 times the recommended NPK, but higher levels of pigments and protein were maintained compared to the recommended NPK under ambient O3 levels. KeywordsAntioxidants-Photosynthetic pigments-Lipid peroxidation-Mustard varieties-NPK levels-Ambient O3
    Water Air and Soil Pollution 04/2012; 214(1):435-450. · 1.63 Impact Factor
  • Article: Differential response of radish plants to supplemental ultraviolet-B radiation under varying NPK levels: chlorophyll fluorescence, gas exchange and antioxidants.
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    ABSTRACT: Current and projected increases in ultraviolet-B (UV-B; 280-315 nm) radiation may alter crop growth and yield by modifying the physiological and biochemical functions. This study was conducted to assess the possibility of alleviating the negative effects of supplemental UV-B (sUV-B; 7.2 kJ m⁻² day⁻¹; 280-315 nm) on radish (Raphanus sativus var Pusa Himani) by modifying soil nitrogen (N), phosphorus (P) and potassium (K) levels. The N, P and K treatments were recommended dose of N, P and K, 1.5 times recommended dose of N, P and K, 1.5 times recommended dose of N and 1.5 times recommended dose of K. Plants showed variations in their response to UV-B radiation under varying soil NPK levels. The minimum damaging effects of sUV-B on photosynthesis rate and stomatal conductance coupled with minimum reduction in chlorophyll content were recorded for plants grown at recommended dose of NPK. Flavonoids increased under sUV-B except in plants grown at 1.5 times recommended dose of N. Lipid peroxidation (LPO) also increased in response to sUV-B at all NPK levels with maximum at 1.5 times recommended dose of K and minimum at recommended dose of NPK. This study revealed that sUV-B radiation negatively affected the radish plants by reducing the photosynthetic efficiency and increasing LPO. The plants grown at 1.5 times recommended dose of NPK/N/K could not enhance antioxidative potential to the extent as recorded at recommended dose of NPK and hence showed more sensitivity to sUV-B.
    Physiologia Plantarum 02/2012; 145(3):474-84. · 3.11 Impact Factor
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    Dataset: Greenhouse gas emissions from rice crop with different tillage permutations in rice–wheat system
    Divya Pandey, Madhoolika Agrawal, Jitendra Singh Bohra
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    ABSTRACT: Agric u ltu re, Ec o sy stem s a n d En viro n m en t j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / a g e e Greenhouse gas emissions from rice crop with different tillage permutations in rice–wheat system a b s t r a c t Global agriculture lies in intersection of two inescapable issues of the present times, viz. keeping pace with growing food demand and participating in climate change mitigation efforts. Rice cultivation is a major emitter of greenhouse gases (GHGs) and no tillage of soil is becoming popular due to low economic investments with improved soil conditions. However, how these arrangements will affect GHG emissions need to be quantified. Therefore, monitoring over diverse agricultural practices is essential for optimum utilization of cultivable land and resources, while identifying the chances of emission reductions. We assessed the impacts of four tillage practices in rice–wheat cultivation system on fluxes of GHGs (CH 4 , N 2 O and CO 2) and yield of rice. The tillage practices were tilling of soil before sowing of every crop (RCT-WCT), tillage before sowing of rice but no tillage before sowing of wheat (RCT-WNT), tillage before sowing of wheat but no tillage before sowing of rice (RNT-WCT), and no tillage before sowing of rice as well as wheat (RNT-WNT). Reduction in tillage frequency led to significant reductions in fluxes of CH 4 and N 2 O, but increased CO 2 while permutations of tillage and no tillage influenced grain yield. RNT-WCT produced next to or comparable to the most yielding RCT-WCT. Although it is difficult to select the best performer since no single tillage permutation showed consistent increment in yield with accompanied emission reductions, RNT-WCT, however may be considered as better agricultural practice for the study region. Long term and extensive spatio-temporal monitoring is still required before making any recommendation. It is also essential for understanding the factors that cause declination in yields under no tillage.
  • Article: Assessment of the interactive effects of ambient O(3) and NPK levels on two tropical mustard varieties (Brassica campestris L.) using open-top chambers.
    Poonam Singh, Shalini Singh, S B Agrawal, Madhoolika Agrawal
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    ABSTRACT: Rising O(3) concentrations in agricultural areas have been identified as a significant threat to crop production in Asia including India. The present work reports the results of a field study conducted to assess the usefulness of higher than recommended NPK dose in modifying the physiological, growth, yield, and seed quality responses of two mustard (Brassica campestris L. var. Vardan and Aashirwad) varieties under ambient ozone level at a rural site of India, using open-top chambers. Twelve hourly mean O(3) concentrations ranged between 27.7 and 59.04 ppb during the growth period. Plants in nonfiltered chambers (NFCs) showed reductions in photosynthetic rate, stomatal conductance, and growth parameters compared to the plants in filtered chambers (FCs), but reductions were of lower magnitude at 1.5 times recommended dose of NPK (1.5 RNPK) compared to recommended (RNPK). Yield and seed quality reduced significantly in plants of NFCs compared to FCs at RNPK, but no significant differences were recorded at 1.5 RNPK. There were higher N uptake and N uptake efficiency of plants in FCs compared to NFCs. Nitrogen utilization efficiency increased in Vardan, but decreased in Aashirwad in NFCs compared to FCs suggesting higher capability of N acquisition and utilization under ambient O(3), which led to a less pronounced reduction in the yield of the former than the latter variety. The differential nitrogen utilization efficiency in these varieties may be potentially used as measure of sensitivity characteristics in breeding programs for yield improvement in mustard under the present trend of increase in O(3) concentrations.
    Environmental Monitoring and Assessment 11/2011; 184(10):5863-74. · 1.40 Impact Factor
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    Article: Carbon footprint: current methods of estimation.
    Divya Pandey, Madhoolika Agrawal, Jai Shanker Pandey
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    ABSTRACT: Increasing greenhouse gaseous concentration in the atmosphere is perturbing the environment to cause grievous global warming and associated consequences. Following the rule that only measurable is manageable, mensuration of greenhouse gas intensiveness of different products, bodies, and processes is going on worldwide, expressed as their carbon footprints. The methodologies for carbon footprint calculations are still evolving and it is emerging as an important tool for greenhouse gas management. The concept of carbon footprinting has permeated and is being commercialized in all the areas of life and economy, but there is little coherence in definitions and calculations of carbon footprints among the studies. There are disagreements in the selection of gases, and the order of emissions to be covered in footprint calculations. Standards of greenhouse gas accounting are the common resources used in footprint calculations, although there is no mandatory provision of footprint verification. Carbon footprinting is intended to be a tool to guide the relevant emission cuts and verifications, its standardization at international level are therefore necessary. Present review describes the prevailing carbon footprinting methods and raises the related issues.
    Environmental Monitoring and Assessment 07/2011; 178(1-4):135-60. · 1.40 Impact Factor
  • Article: Assessment of the variability in response of radish and brinjal at biochemical and physiological levels under similar ozone exposure conditions.
    Supriya Tiwari, Madhoolika Agrawal
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    ABSTRACT: The present investigation was done to evaluate the effects of ambient air pollutants on physiological and biochemical characteristics of radish (Raphnus sativa L. var. Pusa Reshmi) and brinjal (Solanum melongena L. var. Pusa hybrid-6) plants grown in open-top chambers with filtered (FCs) and non-filtered (NFCs) treatments at a suburban site in Varanasi, India. Eight hourly mean concentrations of 11.8, 20.8, and 40.8 ppb for SO2, NO2, and O3, respectively, were recorded. O3 was the most significant pollutant affecting the plant performance. Photosynthetic rate and stomatal conductance declined in both the test plants in NFCs as compared to FCs. Lipid peroxidation was higher in NFCs, but the increase was more in radish compared to brinjal. The constitutive levels of the antioxidants as well as their increments upon O3 exposure were of higher magnitude in brinjal as compared to radish. Reduction in Fv/Fm ratio of the plants in NFCs was a regulatory mechanism to cope with the inefficiency of Calvin cycle. The data indicate that O3 triggered the protective mechanisms in plants which resulted in increments in enzymatic and non-enzymatic antioxidants of O3-exposed plants. The variability of the magnitude of responses in radish and brinjal due to O3 stress suggests that radish is more susceptible to ambient O3 injury compared to brinjal.
    Environmental Monitoring and Assessment 04/2011; 175(1-4):443-54. · 1.40 Impact Factor
  • Article: Modification in growth, biomass and yield of radish under supplemental UV-B at different NPK levels.
    Suruchi Singh, Rima Kumari, Madhoolika Agrawal, S B Agrawal
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    ABSTRACT: Growth, biomass, yield and quality characteristics of radish (Raphanus sativus L. var. Pusa Himani) were investigated under supplemental UV-B (sUV-B; 280-320 nm; +7.2 kJ m(-2) d(-1)) radiation at varying levels of soil NPK. Combinations of NPK were recommended, 1.5 times NPK, 1.5 times N and 1.5 times K. sUV-B radiation negatively affected the growth and economic yield with more reductions at 1.5 times recommended NPK, N and K compared to recommended NPK. Total biomass remained unaffected in plants at recommended NPK under sUV-B radiation. At 1.5 times NPK and N more partitioning of biomass to shoot led to reduction in root shoot ratio and consequently yield under sUV-B. Nutrients in edible part declined maximally at 1.5 times recommended K under sUV-B. The study suggests that higher than recommended NPK makes radish plants more sensitive to sUV-B in terms of yield by allocating less photosynthates towards roots compared to shoots.
    Ecotoxicology and Environmental Safety 01/2011; 74(4):897-903. · 2.29 Impact Factor
  • Article: Tropospheric ozone and plants: absorption, responses, and consequences.
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    ABSTRACT: Ozone is now considered to be the second most important gaseous pollutant in our environment. The phytotoxic potential of O₃ was first observed on grape foliage by B.L. Richards and coworkers in 1958 (Richards et al. 1958). To date, unsustainable resource utilization has turned this secondary pollutant into a major component of global climate change and a prime threat to agricultural production. The projected levels to which O₃ will increase are critically alarming and have become a major issue of concern for agriculturalists, biologists, environmentalists and others plants are soft targets for O₃. Ozone enters plants through stomata, where it disolves in the apoplastic fluid. O₃ has several potential effects on plants: direct reaction with cell membranes; conversion into ROS and H₂O₂ (which alters cellular function by causing cell death); induction of premature senescence; and induction of and up- or down-regulation of responsive components such as genes , proteins and metabolites. In this review we attempt to present an overview picture of plant O₃ interactions. We summarize the vast number of available reports on plant responses to O₃ at the morphological, physiological, cellular, biochemical levels, and address effects on crop yield, and on genes, proteins and metabolites. it is now clear that the machinery of photosynthesis, thereby decreasing the economic yield of most plants and inducing a common morphological symptom, called the "foliar injury". The "foliar injury" symptoms can be authentically utilized for biomonitoring of O₃ under natural conditions. Elevated O₃ stress has been convincingly demonstrated to trigger an antioxidative defense system in plants. The past several years have seen the development and application of high-throughput omics technologies (transcriptomics, proteomics, and metabolomics) that are capable of identifying and prolifiling the O₃-responsive components in model and nonmodel plants. Such studies have been carried out ans have generated an inventory of O₃-Responsive components--a great resource to the scientific community. Recently, it has been shown that certain organic chemicals ans elevated CO₂ levels are effective in ameliorating O₃-generated stress. Both targeted and highthroughput approaches have advanced our knowledge concerning what O₃-triggerred signaling and metabolic pathways exist in plants. Moreover, recently generated information, and several biomarkers for O₃, may, in the future, be exploited to better screen and develop O₃-tolerant plants.
    Reviews of environmental contamination and toxicology 01/2011; 212:61-111. · 3.45 Impact Factor
  • Article: Investigating the impact of elevated levels of ozone on tropical wheat using integrated phenotypical, physiological, biochemical, and proteomics approaches.
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    ABSTRACT: Ozone (O3), a potent air pollutant and a significant greenhouse gas, has been recognized as a major component of global climate change. However, current increasing trends in its background level are projecting a more severe threat to natural and cultivated plants in the near future. The present study has been designed to evaluate the impact of elevated concentrations of O3 on phenotypical, physiological, and biochemical traits in two high-yielding cultivars of wheat, followed by analysis of the leaf proteome using one/two-dimensional gel electrophoresis (1-/2-DGE) coupled to immunoblotting and mass spectrometry analyses under near-natural conditions using open top chambers. Prominently, O3 exposure caused specific foliar injury in both the wheat cultivars. Results also showed that O3 stress significantly decreased photosynthetic rate, stomatal conductance, and chlorophyll fluorescence kinetics (Fv/Fm) in test cultivars. Biochemical evaluations further revealed a higher loss in photosynthetic pigments, whereas a significantly induced antioxidant system under elevated O3 concentrations pointed toward an ability of O3 to generate oxidative stress. 1-DGE analysis showed drastic reductions in the abundantly present ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) large and small subunits. Western blot analysis confirmed induced accumulation of antioxidative enzymes like superoxide dismutase and ascorbate peroxidase protein(s) and common defense/stress-related thaumatin-like protein(s). 2-DGE analysis revealed a total of 38 differentially expressed protein spots, common in both the wheat cultivars. Among those, some major leaf photosynthetic proteins (including RuBisCO and RuBisCO activase) and important energy metabolism proteins (including ATP synthase, aldolase, and phosphoglycerate kinase) were drastically reduced, whereas some stress/defense-related proteins (such as harpin-binding protein and germin-like protein) were induced. In all, the present study reveals an intimate molecular network provoked by O3 affecting photosynthesis and triggering antioxidative defense and stress-related proteins culminating in accelerated foliar injury in wheat plants.
    Journal of Proteome Research 09/2010; 9(9):4565-84. · 5.11 Impact Factor
  • Article: Effectiveness of different EDU concentrations in ameliorating ozone stress in carrot plants.
    Supriya Tiwari, Madhoolika Agrawal
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    ABSTRACT: Ethylenediurea (EDU) is suggested for use to evaluate plant response under ambient ozone (O(3)) concentrations. Four EDU treatments, viz. 0 (non-EDU), 150, 300 and 450 mg L(-1), applied as soil drench at 10 days interval to carrot (Daucus carota L. var. Pusa Kesar), grown at a tropical suburban site of Varanasi experiencing mean O(3) concentration of 36.1 ppb during the experimental period. EDU treated plants showed significantly higher antioxidative defense, assimilation capability and reduced membrane lipid peroxidation, which led to better growth and significant yield increments compared to non-EDU treated ones. The magnitude of positive responses was highest at 150 mg L(-1) EDU treatment at 60 DAG, representing the metabolically most active phase of root filling in carrot. This study suggests that the lowest EDU concentration was sufficient to provide protection against negative effects of O(3).
    Ecotoxicology and Environmental Safety 07/2010; 73(5):1018-27. · 2.29 Impact Factor
  • Article: Effects of Wastewater Irrigation on Physicochemical Properties of Soil and Availability of Heavy Metals in Soil and Vegetables
    Anita Singh, Rajesh K. Sharma, Madhoolika Agrawal, F. Marshall
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    ABSTRACT: The present study investigated the impact of irrigation with wastewater on nutritional property and heavy‐metal concentrations in the soil and consequent accumulation in vegetables at sites having long‐term uses of wastewater for irrigation. Samples of irrigation water, soil, and root and shoot parts of palak plants were analyzed to determine the concentration of heavy metals. Wastewater irrigation led to increases in the total and phytoavailable heavy‐metal concentrations in the soil at all the sites. Heavy‐metal concentrations in soil under wastewater irrigation were negatively and positively correlated with soil hydrogen potential (pH) and organic carbon (OC), respectively. The enrichment factor and metal pollution index were higher at wastewater‐irrigated sites as compared to the clean water–irrigated ones. The study concludes that wastewater irrigation modified the physicochemical properties of the soil, leading to more availability of heavy metals in the soil and consequently to the plant.
    Communications in Soil Science and Plant Analysis 12/2009; 40(21-22):3469-3490. · 0.51 Impact Factor
  • Article: Differential protection of ethylenediurea (EDU) against ambient ozone for five cultivars of tropical wheat.
    Shalini Singh, S B Agrawal, Madhoolika Agrawal
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    ABSTRACT: The antiozonant EDU (ethylenediurea) was used to assess the impact of ambient O(3) under field conditions on five cultivars of tropical wheat (Triticum aestivum L.). EDU solution (0 ppm and 400 ppm) was applied as soil drench (100 ml plant(-1)) 10 days after germination (DAG) at an interval of 12 days. EDU-treated plants showed significant increments in stomatal conductance, photosynthetic rate, variable fluorescence, total chlorophyll, ascorbic acid, proline and protein contents and protective enzymes (POX, SOD and APX) activities in HUW468, HUW510 and HUW234 cultivars, while, a reverse trend was observed for lipid peroxidation. EDU application restored grain yield significantly by maintaining higher levels of antioxidants, metabolites and enzymes in cultivars HUW468 and HUW510. Sonalika and PBW343 showed least response of measured parameters under EDU treatment suggesting their greater resistance to O(3). EDU, thus proved its usefulness in screening suitable wheat cultivars for areas experiencing elevated concentrations of O(3).
    Environmental pollution (Barking, Essex: 1987) 05/2009; 157(8-9):2359-67. · 3.43 Impact Factor
  • Article: Protection of palak (Beta vulgaris L. var Allgreen) plants from ozone injury by ethylenediurea (EDU): roles of biochemical and physiological variations in alleviating the adverse impacts.
    Supriya Tiwari, Madhoolika Agrawal
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    ABSTRACT: Ameliorative effects of ethylenediurea (N-[2-(2-oxo-1-imidazolinidyl) ethyl]-N' phenylurea, abbreviated as EDU) against ozone stress were studied on selected growth, biochemical, physiological and yield characteristics of palak (Beta vulgaris L. var Allgreen) plants grown in field at a suburban site of Varanasi, India. Mean eight hourly ozone concentration varied from 52 to 73 ppb which was found to produce adverse impacts on plant functioning and growth characteristics. The palak plants were treated with 300 ppm EDU at 10 days after germination at 10 days interval up to the plant maturity. Lipid peroxidation in EDU treated plants declined significantly as compared to non-EDU treated ones. Significant increment in F(v)/F(m) ratio in EDU treated plants as compared to non-EDU treated ones was recorded. EDU treated plants showed significant increment in ascorbic acid contents and reduction in peroxidase activity as compared to non-EDU treated ones. As a result of the protection provided by EDU against ozone induced stress on biochemical and physiological characteristics of palak, the morphological parameters also responded positively. Significant increments were recorded in shoot length, number of leaves plant(-1), leaf area and root and shoot biomass of EDU treated plants as compared to non-EDU treated ones. Contents of Na, K, Ca, Mg and Fe were higher in EDU treated plants as compared to non-EDU treated ones. The present investigation proves the usefulness of EDU in partially ameliorating ozone injury in ambient conditions.
    Chemosphere 04/2009; 75(11):1492-9. · 3.21 Impact Factor
  • Article: Evaluation of physiological and biochemical responses of two rice (Oryza sativa L.) cultivars to ambient air pollution using open top chambers at a rural site in India.
    Richa Rai, Madhoolika Agrawal
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    ABSTRACT: Air pollutant concentrations are rising in India, causing potential threats to crop production. As air pollutants are known to interfere with physiological processes, this study was conducted to assess the relative responses of physiological and biochemical characteristics of two cultivars of rice (Oryza sativa L. cv. Saurabh 950 and NDR 97) leading to variable yield responses. Twelve hour monitoring of ambient concentrations of SO2, NO2 and O3 in filtered chambers (FCs), non-filtered chambers (NFCs) and open plots (OPs) showed that O3 was the main pollutant at the experimental site. Ozone concentrations often exceeded 40 ppb during anthesis but not during the vegetative growth period. Photosynthetic rate (Ps), stomatal conductance (g(s)) and Fv/Fm ratio, superoxide dismutase (SOD) and peroxidase (POD) activities and photosynthetic pigments, ascorbic acid, total phenolics and protein contents were assessed at different developmental stages and yield of grains were quantified. Lipid peroxidation, SOD and POD activities, ascorbic acid and total phenolics were higher, whereas Ps, g(s), Fv/Fm ratio and contents of protein and photosynthetic pigment were lower in plants of NFCs as compared to FCs. Yield decreased significantly in both cultivars grown in NFCs. NDR 97 showed less reductions in physiological characteristics, photosynthetic pigments and protein, but a greater increase in the antioxidative defense system as compared to Saurabh 950. Yield reduction was higher in NDR 97 than in Saurabh 950. This suggested that NDR 97 utilized more photosynthate in maintaining the metabolic machinery against O3 stress leading to lower translocation of photosynthate to reproductive parts. The study concluded that under natural field conditions, physiological and biochemical responses of plants varied with pollutant concentrations leading to different translocation strategies in plants, modifying their yield responses. NDR 97, a fast growing and high yielding cultivar was more sensitive than slow growing Saurabh 950.
    Science of The Total Environment 01/2009; 407(1):679-91. · 3.29 Impact Factor
  • Article: Heavy metals in vegetables collected from production and market sites of a tropical urban area of India.
    Rajesh Kumar Sharma, Madhoolika Agrawal, Fiona M Marshall
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    ABSTRACT: Vegetables (Beta vulgaris L., Abelmoschus esculentus L. and Brassica oleracea L.) from the production and market sites of India were tested for Cu, Cd, Zn and Pb. At market sites, the mean concentration of Cu in cauliflower, and of Zn and Cd in both palak and cauliflower had exceeded the PFA standard. Zn at the production sites also exceeded the PFA standard in cauliflower. Cd concentration in vegetables tested from both production and market sites was many folds higher than the EU standard. In contrast, Pb in vegetables tested from both production and market sites was below the PFA limit, but was considerably higher than the current EU and WHO standards. Heavy metals accumulation in vegetables tested are higher at market sites than those at the crop production sites. The contributions of these vegetables to dietary intake of Cu, Zn, Cd and Pb were 13%, 1%, 47% and 9% of provisional tolerable daily intake, respectively. The study concludes that the transportation and marketing systems of vegetables play a significant role in elevating the contaminant levels of heavy metals which may pose a threat to the quality of the vegetables with consequences for the health of the consumers of locally produced foodstuffs.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 01/2009; 47(3):583-91. · 2.99 Impact Factor
  • Article: Atmospheric deposition of heavy metals (Cu, Zn, Cd and Pb) in Varanasi City, India.
    Rajesh Kumar Sharma, Madhoolika Agrawal, Fiona M Marshall
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    ABSTRACT: Rapid growth in urbanization and industrialization in developing countries may significantly contribute in heavy metal contamination of vegetables through atmospheric depositions. In the present study, an assessment was made to investigate the spatial and seasonal variations in deposition rates of heavy metals and its contribution to contamination of palak (Beta vulgaris). Samples of bulk atmospheric deposits and Beta vulgaris for analysis of Cu, Zn, Cd and Pb were collected from different sampling locations differing in traffic density and land use patterns. The results showed that the sampling locations situated in industrial or commercial areas with heavy traffic load showed significantly elevated levels of Cu, Zn and Cd deposition rate as compared to those situated in residential areas with low traffic load. The deposition rates of Cu, Zn and Cd were significantly higher in summer and winter as compared to rainy season, however, Pb deposition rate was significantly higher in rainy and summer seasons as compared to winter season. Atmospheric depositions have significantly elevated the levels of heavy metals in B. vulgaris collected during evening as compared to those collected in morning hours. The study further showed that local population has maximum exposure to Cd contamination through consumption of B. vulgaris. The present study clearly points out the urban and industrial activities of a city have potential to elevate the levels of heavy metals in the atmospheric deposits, which may consequently contaminate the food chain and thus posing health risk to the local population.
    Environmental Monitoring and Assessment 08/2008; 142(1-3):269-78. · 1.40 Impact Factor
  • Article: Heavy metal (Cu, Zn, Cd and Pb) contamination of vegetables in urban India: a case study in Varanasi.
    Rajesh Kumar Sharma, Madhoolika Agrawal, Fiona M Marshall
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    ABSTRACT: The contributions of heavy metals in selected vegetables through atmospheric deposition were quantified in an urban area of India. Deposition rate of Zn was recorded maximum followed by Cu, Cd and Pb. The concentrations of Zn and Cu were highest in Brassica oleracea, Cd in Abelmoschus esculentus and B. oleracea, while Pb was highest in Beta vulgaris. Heavy metal pollution index showed that B. oleracea was maximally contaminated with heavy metals followed by A. esculentus and then B. vulgaris. The results of washing showed that atmospheric deposition has contributed to the increased levels of heavy metals in vegetables. Both Cu and Cd posed health risk to local population via test vegetables consumption, whereas Pb posed the same only through B. oleracea. The study concludes that atmospheric depositions can elevate the levels of heavy metals in vegetables during marketing having potential health hazards to consumers.
    Environmental Pollution 08/2008; 154(2):254-63. · 3.75 Impact Factor
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    Article: Acid rain and its ecological consequences.
    Anita Singh, Madhoolika Agrawal
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    ABSTRACT: Acidification of rain-water is identified as one of the most serious environmental problems of transboundary nature. Acid rain is mainly a mixture of sulphuric and nitric acids depending upon the relative quantities of oxides of sulphur and nitrogen emissions. Due to the interaction of these acids with other constituents of the atmosphere, protons are released causing increase in the soil acidity Lowering of soil pH mobilizes and leaches away nutrient cations and increases availability of toxic heavy metals. Such changes in the soil chemical characteristics reduce the soil fertility which ultimately causes the negative impact on growth and productivity of forest trees and crop plants. Acidification of water bodies causes large scale negative impact on aquatic organisms including fishes. Acidification has some indirect effects on human health also. Acid rain affects each and every components of ecosystem. Acid rain also damages man-made materials and structures. By reducing the emission of the precursors of acid rain and to some extent by liming, the problem of acidification of terrestrial and aquatic ecosystem has been reduced during last two decades.
    Journal of Environmental Biology 02/2008; 29(1):15-24. · 0.64 Impact Factor