Andrew Bottrill

Publications (2)13.22 Total impact

• Source

  Available from: pnas.org

  Article: The twin-arginine translocation pathway is a major route of protein export in Streptomyces coelicolor.

  David A Widdick, Kieran Dilks, Govind Chandra, Andrew Bottrill, Mike Naldrett, Mechthild Pohlschröder, Tracy Palmer
  [show abstract] [hide abstract]
  ABSTRACT: The twin-arginine translocation (Tat) pathway is a protein transport system for the export of folded proteins. Substrate proteins are targeted to the Tat translocase by N-terminal signal peptides harboring a distinctive R-R-x-Phi-Phi "twin-arginine" amino acid motif. Using a combination of proteomic techniques, the protein contents from the cell wall of the model Gram-positive bacterium Streptomyces coelicolor were identified and compared with that of mutant strains defective in Tat transport. The proteomic experiments pointed to 43 potentially Tat-dependent extracellular proteins. Of these, 25 were verified as bearing bona fide Tat-targeting signal peptides after independent screening with a facile, rapid, and sensitive reporter assay. The identified Tat substrates, among others, include polymer-degrading enzymes, phosphatases, and binding proteins as well as enzymes involved in secondary metabolism. Moreover, in addition to predicted extracellular substrates, putative lipoproteins were shown to be Tat-dependent. This work provides strong experimental evidence that the Tat system is used as a major general export pathway in Streptomyces.
  Proceedings of the National Academy of Sciences 12/2006; 103(47):17927-32. · 9.68 Impact Factor
• Article: Identification of a MAP65 isoform involved in directional expansion of plant cells.

  Jordi Chan, Guojie Mao, Andrei Smertenko, Patrick J Hussey, Mike Naldrett, Andrew Bottrill, Clive W Lloyd
  [show abstract] [hide abstract]
  ABSTRACT: MAP65 comprises a multigene family specific to plants. To see which isoform is utilised for the unique mechanism of cell expansion, uncomplicated by division structures, carrot cells were deprived of auxin whereupon they stopped dividing and elongated instead. During elongation, a MAP65 protein triplet reduced to a single band. Mass spectrometric analysis demonstrated that this corresponded to a single carrot cDNA; it also corresponded to the major protein previously shown to form filamentous cross-bridges between microtubules in vitro. This MAP65 isoform is concluded to have a major role in establishing the parallel microtubule arrays characteristic of cells undergoing directional expansion.
  FEBS Letters 02/2003; 534(1-3):161-3. · 3.54 Impact Factor