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Journal of Hazardous Materials 05/2013; · 4.17 Impact Factor
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ABSTRACT: Trace element pollution of marine dredged sediments is an emerging problem all over the world. Comparing to other wastes, trace elements stabilization is more difficult both due to the wide range of contaminants present in the marine sediments and their inherent physicochemical properties. In this study, a pilot-scale experiment was performed to stabilize As, Cd, Cu, Mo, Ni, and Zn in a multi-contaminated sediment sample using hematite, zero-valent iron and zeolite. Results showed that iron-based amendments were able to reduce the leaching and the bioavailability of trace elements in the sediment sample, while zeolite was unsuitable. Chemical stabilization through iron-based amendments seems to be a promising approach as a low-cost alternative to traditional stabilization methods involving chemical reagents.
Journal of hazardous materials 03/2013; 252-253C:213-219. · 4.14 Impact Factor
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ABSTRACT: This work investigates arsenic mobility, bioavailability and toxicity in marine port sediments using chemical sequential extraction and laboratory toxicity tests. Sediment samples were collected from two different Mediterranean ports, one highly polluted with arsenic and other inorganic and organic pollutants (Estaque port (EST)), and the other one, less polluted, with a low arsenic content (Saint Mandrier port (SM)). Arsenic distribution in the solid phase was studied using a sequential extraction procedure specifically developed for appraising arsenic mobility in sediments. Toxicity assessment was performed on sediment elutriates, solid phases and aqueous arsenic species as single substance using the embryo-toxicity test on oyster larvae (Crassostrea gigas) and the Microtox test with Vibrio fischeri. Toxicity results showed that all sediment samples presented acute and sub-chronic toxic effects on oyster larvae and bacteria, respectively. The Microtox solid phase test allow to discriminate As-contaminated samples from the less contaminated ones, suggesting that toxicity of whole sediment samples is related to arsenic content. Toxicity of dissolved arsenic species as single substance showed that Vibrio fischeri and oyster larvae are most sensitive to As(V) than As(III). The distribution coefficient (Kd) of arsenic in sediment samples was estimated using results obtained in chemical sequential extractions. The Kd value is greater in SM (450Lkg(-1)) than in EST (55Lkg(-1)), indicating that arsenic availability is higher for the most toxic sediment sample (Estaque port). This study demonstrates that arsenic speciation play an important role on arsenic mobility and its bioavailability in marine port sediments.
Chemosphere 12/2012; · 3.21 Impact Factor
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Jin-Fen Pan,
Pierre-Emmanuel Buffet,
Laurence Poirier,
Claude Amiard-Triquet,
Douglas Gilliland,
Yolaine Joubert,
Paul Pilet,
Marielle Guibbolini,
Christine Risso de Faverney, Michèle Roméo,
Eugenia Valsami-Jones,
Catherine Mouneyrac
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ABSTRACT: Gold nanoparticles (AuNPs) have important technological applications resulting in an increased potential for release to the environment, and a greater possibility of toxicological effects. The marine bivalve Scrobicularia plana was exposed to AuNPs of size 5, 15 and 40 nm during a 16 d laboratory exposure at 100 μg Au L(-1). After exposure to AuNPs forming aggregates (>700 nm), the clams accumulated Au in their soft tissues. Biochemical (biomarkers) and behavioral (burrowing and feeding) responses were investigated. Au NPs were responsible of metallothionein induction (5, 40 nm), increased activities of catalase (15, 40 nm) and superoxide dismutase (40 nm) and of glutathione S-transferase by the three sizes of AuNPs indicating defense against oxidative stress. Exposure to AuNPs impaired burrowing behavior. However, it must be underlined that these effects were observed at a dose much higher than expected in the environment.
Environmental pollution (Barking, Essex: 1987) 05/2012; 168:37-43. · 3.43 Impact Factor
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ABSTRACT: The aim of this work is to assess the potential ecotoxicological effects of contaminated sediments treated with mineral additives. The Microtox solid phase test was used to evaluate the effect of mineral additives on the toxicity of sediment suspensions. Four Mediterranean port sediments were studied after dredging and bioremediation: Sample A from navy harbor, sample B from commercial port and samples C and D from pleasure ports. Sediment samples were stabilized with three mineral additives: hematite, zero-valent iron and zeolite. Results show that all studied mineral additives can act as stabilizer agent in highly contaminated sediments (A and C) by decreasing dissolved metal concentrations and sediment toxicity level. On the contrary, for the less contaminated samples (B and D) hematite and zeolite can provoke toxic effect towards Vibrio fischeri since additive particles can favor bacteria retention and decrease bioluminescence emission.
Chemosphere 12/2011; 86(11):1112-6. · 3.21 Impact Factor
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Desalination 10/2011; · 2.59 Impact Factor
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Pierre-Emmanuel Buffet,
Olivia Fossi Tankoua,
Jin-Fen Pan,
Deborah Berhanu,
Christine Herrenknecht,
Laurence Poirier,
Claude Amiard-Triquet,
Jean-Claude Amiard,
Jean-Baptiste Bérard,
Christine Risso,
Marielle Guibbolini, Michèle Roméo,
Paul Reip,
Eugenia Valsami-Jones,
Catherine Mouneyrac
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ABSTRACT: Engineered nano-sized Cu oxide particles are extensively used in diverse applications. Because aquatic environments are the ultimate "sink" for all contaminants, it is expected that nanoparticles (NP) will follow the same fate. In this study, two marine invertebrates Scrobicularia plana and Hediste diversicolor were chosen as ecotoxicological models. The aim was to evaluate behavioural (burrowing kinetics, feeding rate) and biochemical (biomarkers) responses of S. plana and H. diversicolor exposed in the laboratory to Cu (10 μg L(-1)) added in natural seawater either in the form of engineered nanoparticles (NPs) of CuO or as dissolved Cu in 2% HNO(3). Exposure was characterized by considering (i) the physico-chemical fate of NP (ii) the fraction of labile Cu in experimental media and (iii) Cu bioaccumulation. Results showed high aggregation of CuO NPs in seawater and no additional bioavailable Cu concentrations. Behavioural impairments were observed in S. plana exposed to CuO NPs or soluble Cu whereas in H. diversicolor, only the exposure to soluble Cu led to a burrowing decrease. No obvious neurotoxicity effects were revealed since in both species, no changes in cholinesterasic activity occurred in response to both forms of Cu exposure. Biomarkers of oxidative-stress catalase and glutathione-S-transferase were enhanced in both species whereas superoxide dismutase was increased only in S. plana exposed to CuO NPs. Metallothionein-like protein was increased in bivalves exposed to both forms of Cu. Since, no detectable release of soluble Cu from CuO NPs occurred during the time of experiment, ecotoxicity effects seem to be related to CuO NPs themselves.
Chemosphere 02/2011; 84(1):166-74. · 3.21 Impact Factor
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ABSTRACT: This work investigates the ecotoxicological evaluation of contaminated dredged sediments from French Mediterranean navy harbour (A), commercial port (B) and two composite specimens (C) and (D) coming from the mixture of A and B with other port sediments. The toxicity of elutriates from these sediments is estimated using embryo-toxicity test, Microtox® solid phase test, LuminoTox, phytotoxicity tests and genotoxicity test. Bioassay responses are not clearly correlated with chemical contamination in the whole sediment and vary as a function of tested organisms. The highest contaminated samples (A and C) are almost always more toxic than the less contaminated samples (B and D). Among composite sediments, the mixture effect with other sediments is not efficient to decrease toxicity in sample C, suggesting that other parameters influence toxicity level such as particle size or organic matter content. These parameters should be taken into consideration in order to improve the efficiency of the mixture process and produce composite sediments with low toxicity.
Chemosphere 10/2010; 82(3):362-9. · 3.21 Impact Factor
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ABSTRACT: Ubiquitination and carbonylation of proteins were investigated in the gill and digestive gland of Ruditapes decussatus exposed to NP (nonylphenol) (100 μgL(-1)) using redox proteomics. After 21 d of exposure, clams were dissected and cytosolic proteins of both tissues separated by 2DE SDS-PAGE. Protein expression profiles were tissue-dependent and differently affected by NP exposure. Ubiquitination and carbonylation were also tissue-specific. NP exposure induced significantly more ubiquitinated proteins in gills than in digestive glands, compared to controls. Digestive gland showed a significant higher number of carbonylated proteins than gills after NP exposure. Protein ubiquitination and carbonylation are therefore independent processes. Results showed that NP exposure generated ROS in gill and digestive gland of R. decussatus that significantly altered the proteome. Results also highlighted the advantage of using redox proteomics in the assessment of protein ubiquitination and carbonylation, which may be markers of oxidative stress in R. decussatus.
Chemosphere 10/2010; 81(10):1212-7. · 3.21 Impact Factor
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ABSTRACT: The ecotoxicological effect of dredged sediments was estimated by embryo-larval toxicity of the oyster Crassosstrea gigas in sediment elutriates (filtered and unfiltered). The study covers the main ports from the French Mediterranean coast. Composted sediments from a navy harbour (A), a commercial port (B) and two composite specimens (C and D) obtained after mixing various sediments were taken into consideration. Effective concentrations affecting 50% of larvae (EC50) were obtained from different elutriate concentrations (from 0 to 100%). Toxicity results obtained from filtered elutriates decreased according to the following gradient: sample A (5.68%), B (20.50%), C (37.60%) and D (47.17%). Chemical concentrations in whole sediments were in agreement with those in elutriates. Among the measured contaminants in elutriates, Cu and Zn resulted as the main contributors to toxicity. Dissolved organic carbon played an important role by exerting a protective effect against the toxicity of dissolved Cu. Toxicity results were interpreted on the basis of toxicity scores to give indication about sediment quality which provided more severe judgement than risk score based on chemical concentrations in sediments.
Water Research 03/2010; 44(6):1986-94. · 4.86 Impact Factor
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European Journal of Environmental and Civil Engineering 01/2010; 14(2):233-251. · 0.32 Impact Factor
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ABSTRACT: The effect of copper chloride was studied on the hepatic microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity of the fish Dicentrarchus labrax intraperitoneally injected with benzo[a]pyrene (BaP). In vitro experiments showed that copper significantly decreased EROD activity, and IC50 was estimated at 50 μg Cu/L. The apparent Michaelis constant (Km) of cytochrome P4501A was constant, whereas maximum velocity (Vmax) decreased as a function of copper added to the incubation medium. (Km) of nicotinamide adenine dinucleotide phosphate [NADPH]-cytochrome P450 reductase increased as a function of copper concentration, whereas Vmax remained constant. Absorption spectra showed that the amount of cytochrome P420s increased as a function of copper concentrations added to the medium at the expense of cytochrome P450s. The injection of copper and BaP (in vivo experiments) to fish decreased EROD activity compared with the injection of BaP alone. An increase of immunoquantified CYP1A content measured by Western blotting (intense band of 55–60 kDa molecular weight) was noted in microsomes of fish injected with BaP compared with controls. In the case of fish treated with copper and BaP, the band was less intense and accompanied by another band of lower molecular weight. The destruction of the native P450s spectrophotometrically measured in the presence of copper implied that the catalytic activity would be diminished. This was confirmed by decreased EROD activity after either in vitro additions or in vivo treatment with copper. Moreover, immunodetection experiments suggested that the decrease of the catalytic activity resulted more from cytochrome P450s loss than from direct inhibition of EROD activity by copper.
Environmental Toxicology and Chemistry 10/2009; 16(2):214 - 219. · 2.81 Impact Factor
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ABSTRACT: Cadmium, an environmental stressor due to its toxicity, persistence and accumulation in biota, is widespread in the aquatic environment. Cadmium accumulation kinetics have revealed that Ruditapes decussatus has a high affinity to this metal. Proteomics is an effective tool to evaluate the toxic effects of contaminants. The aim of this study was to investigate the Cd effects in the gill and digestive gland of the sentinel species R. decussatus. Protein expression profiles (PEPs) in the clam tissues exposed to Cd (40 microg l(-1), 21 days) were compared to unexposed ones. Cd induces major changes in tissue-specific protein expression profiles in gill and digestive gland. This tissue dependent response results mainly from differences in Cd accumulation, protein inhibition and/or autophagy. An overall decrease of protein spots was detected in both treated tissues, being higher in gill. Some of the spots more drastically altered after pollutants exposure were excised and nine were identified by micro liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins identified by homology search in databases included: three proteins (8-fold) up-regulated, one down-regulated, four suppressed and one induced. Cd induces major changes in proteins involved in cytoskeletal structure maintenance (muscle-type actin, adductor muscle actin and beta-tubulin), cell maintenance (Rab GDP) and metabolism (ALDH and MCAD, both identified by de novo sequencing) suggesting potential energetic change. They provide a valuable knowledge of Cd effects at biochemical and molecular levels in the gill and digestive gland of R. decussatus.
Aquatic toxicology (Amsterdam, Netherlands) 08/2009; 94(4):300-8. · 3.12 Impact Factor
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Comptes Rendus Chimie 08/2009; · 1.80 Impact Factor
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ABSTRACT: Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can be applied to these organisms, especially for Ruditapes decussatus and Bathymodiolus azoricus.
Methods in molecular biology (Clifton, N.J.) 02/2009; 519:197-204.
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Human and Ecological Risk Assessment 01/2009; 15:11-26. · 1.54 Impact Factor
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ABSTRACT: Environmental pollutants, such as metals, are widespread in aquatic environments and can lead to the formation of reactive oxygen species (ROS). ROS are highly toxic in marine species since they can cause serious reversible and irreversible changes in proteins including ubiquitination and modifications such as carbonylation. This study aimed to confirm the potential of ubiquitination and carbonylation as markers of oxidative stress in the clam Ruditapes decussatus (Veneroida, Veneridae) exposed to cadmium (40 microg/L). After 21 days of exposure clams were dissected into gills and digestive gland. Cytosolic proteins of both tissues were separated by two-dimensional electrophoresis (2-D SDS-PAGE) and analysed by immunobloting. Higher ubiquitination and carbonylation levels were in digestive gland of contaminated organisms. These results confirm the potential of ubiquitination and carbonylation as a sensitive and specific marker of oxidative stress in marine bivalves. In this approach, changes in protein structure provide options for affinity selection of sub-proteomes for 2D SDS-PAGE, simplifying the detection of protein biomarkers using proteomic approach.
Marine Environmental Research 08/2008; 66(1):95-7. · 2.28 Impact Factor
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ABSTRACT: Relationships between biochemical and physiological biomarkers (acetylcholinesterase [AChE], catalase, and glutathione S-transferase [GST] activities, thiobarbituric acid reactive substances, glycogen, lipids and proteins) and accumulated concentrations of contaminants (polychlorinated biphenyls [PCBs], polycyclic aromatic hydrocarbons and metals) were examined in the keystone species Nereis diversicolor. The chemical analyses of worms and sediments allowed the designation of the Seine estuary and the Authie estuary as a polluted and relatively clean site respectively. Worms from the Seine estuary exhibited higher GST and lower AChE activities. Generally, larger worms had higher concentrations of energy reserves. Principal component analyses clearly highlighted intersite differences: in the first plan, GST activities and chemical concentrations were inversely related to concentrations of energy reserves; in the second one, PCB concentrations and AChE activity were inversely related. Depleted levels of energy reserves could be a consequence of combating toxicants and might predict effects at higher levels of biological organization. The use of GST and AChE activities and energy reserve concentrations as biomarkers is validated in the field in this keystone species.
Environmental Pollution 08/2007; 148(2):445-58. · 3.75 Impact Factor
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ABSTRACT: Suborganismal responses to toxicants can be sensitive tools to assess marine pollution, but their ecological significance is a matter of debate. Among these biomarkers, those linked to reproduction are most probably related to populational effects. To test this hypothesis, Nereis diversicolor were collected in the multipolluted Seine estuary and the comparatively clean Authie estuary (France). Energy reserves were higher in Authie worms, suggesting a better physiological status. The number of oocytes per female was higher for the polychaetes from the Authie, but it was related to the size of animals, which was higher at this site. Densities of worms were depleted in the Seine compared to those in Authie. Demographic structure of the Seine population was also altered. The concomitant changes in energy reserves, egg production, and population structure and density suggest that the effects on biomarkers and at the population level are related.
Ecotoxicology and Environmental Safety 04/2007; 66(3):402-11. · 2.29 Impact Factor
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ABSTRACT: Glutathione S-transferase is involved in the detoxication of many chemical compounds. Northern blot analysis of mRNA GST gene from the mussel Mytilus galloprovincialis shows the presence of a transcript of 850 bp (GenBank accession no. Gi:22094808 and AF527010). The cDNA cloned sequence is constituted by an ORF of 621 bp encoding for a protein of 23,700 Da present in the gills and digestive gland of M galloprovincialis. The sequence, called Mg (M. galloprovincialis) GST-p, is clearly related to the pi class GST. M. galloprovincialis treated with Cd (200 microg/L) and BaP (100 microg/L) or co-treated with Cd and BaP take up cadmium in the gills (16.2 +/- 4.2 and 12.6 +/- 1.2 microg Cd/g dry wt. after exposure to Cd and Cd + BaP, respectively) and in the digestive gland. The transcription of GST-pi gene, by semi-quantitative RT-PCR, shows the lowest value in the digestive glands of mussels exposed to BaP, whereas the treatment with cadmium and the co-treatment with cadmium and BaP evoke GST-pi gene expression higher than controls. Mussels collected from six sites along the south coast of Portugal show different GST-pi transcription levels, some of which are related to their pollutant content.
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology 07/2006; 143(2):196-203. · 2.62 Impact Factor
