Nezahat Akpolat

Dicle University, Amida, Diyarbakır, Turkey

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Publications (8)9.44 Total impact

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    ABSTRACT: The Ziehl-Neelsen (ZN) and fluorochrome (FC) staining procedures and the Bactec and Löwenstein-Jensen (L-J) culturing procedures were used to test 340 clinical specimens for tuberculosis, and the diagnostic value of the fluorochrome procedure was investigated. Positive cultures were obtained from 34 specimens (10%), of which 13 (3.8%) tested positive in ZN, and 18 (5.3%) in FC. Sensitivity of the ZN and FC staining results was found to be 38.2% and 52.9%, respectively. NAP (p-nitro-α-acetylamino-β-hidroxypropiophenone) identified 32 (94.1%) of the 34 strains as M. tuberculosis complex, and 2 (5.9%) as Mycobacteria other than tuberculosis (MOTT) bacilli. Twenty-one (61.7%) of the 34 culture-positive specimens grew only in Bactec 12 B medium, 2 (5.9%) grew only in L-J medium, and 11 (32.3%) grew in both Bactec and L-J media. The 32 M. tuberculosis complex strains' sensitivities to streptomycin (STR), isoniazid (INH), rifampin (RIF), and ethambutol were assessed with the Bactec system. Four (12.5%) of these strains were resistant to streptomycin, 9 (28.1%) to isoniazid, 7 (21.8%) to rifampin, and 6 (18.7%) to ethambutol. Total drug resistance was 43.7%. Six strains (18.7%) were resistant to 1 drug, 5 (15.6%) to 2 drugs, 2 (6.2%) to 3 drugs, and 1 (3.1%) to all 4 drugs, isoniazid plus rifampin resistance was seen in 18.7%.
    Biotechnology & Biotechnological Equipment 04/2014; 18(3):165-169. DOI:10.1080/13102818.2004.10817139 · 0.38 Impact Factor
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    ABSTRACT: IgG avidity tests, which came on the scene recently with regard to most efficient use of time and early onset to the therapy, are considered in this study, and compared with En-zyme immunoassay(EIA). Serums of a total of 879 women in the first trimester of their gestations who referred to Dicle University, Faculty Hospital, Department of Gynaeco-logy and Obstetrics in between the dates of 09 April 2002 – 09 May 2003 were evaluated with the classical method ELISA (Cobas Core II, Roche, USA), in terms of Rubella and HCMV IgG and IgM. Avidity tests were performed with (IgG avidity EIA. Well; Radim, Italy) commercial kits. 4 samples among the 8 that were positive for HCMV IgM and IgG were found to have low AIs (AI<35%), 2 samples to have intermediate AIs (AI 35-45%), and the other 2 to have high AIs (AI >45%). One of the 156 IgG positive serum samples had an intermediate AI (AI 35-45%), and 155 had high AIs. In the Rubella IgG avidity tests, 9 of the 13 IgM and IgG positive samples had low AIs (AI <50%), 1 had an interme-diate AI (AI 50-60%), and 3 had high AIs (AI >60%). Only one of the 151 IgG positive samples had a low AI, whereas the remaining 150 had high AIs. If only avidity test is taken into consideration, gray region or low avidity test results especially negative for IgM with ELISA and showing a clear chronical pattern will be falsely interpreted in ac-cord with an infection, if the results for these serums are new. IgG avidity test is useful in serums revealing suspected results in IgM ELISA test. Follow-up of patients with interme-diate and low AIs and repeating tests in certain intervals, and examination of the amniotic fluid by PCR will be appropriate. As only IgG avidity test taken into consideration will cause unnecessary abortus and anxiety, use of single confirmation tests in such cases is not right. IgG avidity tests should only be used as confirmation tests in pregnant women.
    Biotechnology & Biotechnological Equipment 04/2014; 19(3). DOI:10.1080/13102818.2005.10817242 · 0.38 Impact Factor
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    ABSTRACT: The objective of this study was to evaluate the correlation between serum hepatitis D -delta- virus (HDV) RNA detection and anti-HDV IgG and IgM antibodies, in the serodiagnosis of delta hepatitis. A total of 153 HBsAg positive sera were screened for the presence of anti-HBc IgM, anti-HDV IgG and anti-HDV IgM by commercial enzyme immunoassays and HDV-RNA by real time polymerase chain reaction (PCR). Of 153 sera, 86 (56.2%) were found positive for HDV antibodies. Although isolated anti-HDV IgG was present in 35 and isolated anti-HDV IgM was present in 11 patients, IgG and IgM were present concurrently in 40 additional patients. HDV-RNA was detected in 21.5% (33/153) of the patients. Four of the 33 HDV-RNA positive patients were positive only for anti-HDV IgG, 8 were positive only for anti-HDV IgM, and 19 were positive for both anti-HDV IgG and IgM antibodies. Twenty seven of 51 (53%) anti-HDV IgM positive patients were also found positive for HDV-RNA, while 27 of 33 (82%) HDV-RNA positive patients exhibited anti-HDV IgM positivity. Increased serum ALT levels were detected approximately in 85% (28/33) of viremic patients. As all of the HDV-RNA positive patients were found negative for anti-HBc IgM, superinfection with delta virus were considered. In conclusion, PCR is a sensitive and useful method for the detection of viremic patients as well as for the monitorization of antiviral therapy, anti-HDV IgM positivity together with increased ALT levels appear to be good markers for the prediction of hepatitis delta viremia, especially in the countries with limited economical sources as Turkey.
    Mikrobiyoloji bülteni 08/2005; 39(3):345-9. · 0.44 Impact Factor
  • Saudi medical journal 05/2005; 26(4):682-3. · 0.55 Impact Factor
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    ABSTRACT: In this study; we compared the direct microscopic method and EIA test in the investigation of the stools of patients with gastrointestinal symptoms who presented at clinics. A total of 188 stool specimens collected from clinics were investigated by direct microscopy using native-Lugol preparations. Giardia cysts and/or trophozoites were observed in 141 specimens. There were no Giardia cysts and/or trophozoites or any other intestinal parasites detected in the other 47 stool specimens. The RIDASCREENR EIA kit procedure was applied in all specimens. Out of 141 specimens positive with direct microscopy, 136 specimens were positive with the EIA test and 5 specimens, negative. Parasites were not found in 47 stool specimens with direct microscopy. Of these, 38 specimens were negative with the EIA test and 9 specimens, positive. When the patient and control groups were compared, a significant difference was observed between the two methods (p < 0.05). The sensitivity and specificity of the EIA method that was used to determine the antigenic properties of G. intestinalis in stools were 96.4% and 80.8%, respectively.
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 01/2005; 29(2):89-92.
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    ABSTRACT: The reported prevalence of hepatitis C virus (HCV) infection in patients with lichen planus (LP) shows variations from different regions. The aim of this study was to investigate the prevalence of HCV infection in patients with LP, in Diyarbakir region of Turkey. A total of 128 patients with LP and 128 healthy controls were detected for HCV infection, using a third generation enzyme-linked immunosorbent assay. Eight of 128 patients (6.25%) with LP were found to have anti-HCV antibodies, whereas only one patient (0.78%) in control group was found to have anti-HCV antibodies. A statistically significant difference was found between LP and control group (p < 0.001). In conclusion, the coexistence of HCV infection and lichen planus is more than coincidental, and it is appropriate to screen all patients with lichen planus for HCV infection.
    International Journal of Clinical Practice 12/2004; 58(12):1118-9. DOI:10.1111/j.1742-1241.2004.00214.x · 2.54 Impact Factor
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    ABSTRACT: The purpose of this study was to investigate the association of the varicella zoster virus (VZV) IgG, and IgM antibodies with Behçet's and other skin diseases (group 1: recurrent aphthos stomatitis, fungal infections, psoriasis; group 2: vitiligo, lichen planus). Twenty eight patients with Behçet's disease (BD), and 117 patients with dermatological disorders other than BD were evaluated for specific VZV IgG and IgM antibodies by using a third generation enzyme-linked immunosorbent assay (ELISA). The Mantel Heizshel chi2 method was used to adjust the confounding of age and sex of the patients. The serological positivity for VZV IgG and IgM antibodies in BD was not statistically different from other skin diseases. When we considered the age of the patients, chi2 = 2.64, CI (0.27-1.65), odds ratio (1, 1.25, 2.21) (p = 0.10) and when we considered the sex of the patients, chi2 = 0.31, CI (0.81-1.28), odds ratio (1, 1.45, 1.41), (p = 0.57).
    European Journal of Epidemiology 02/2003; 18(1):91-3. DOI:10.1023/A:1022537209948 · 5.15 Impact Factor
  • Selahattin ATMACA, Nezahat AKPOLAT, Kadri GÜL
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    ABSTRACT: Results: In respiratory samples, sensitivity of EZN was found as 83.33%, specificity as 95.04%, positive predictive value as 50%, and negative predictive value as 98.96%, whereas in nonrespiratory samples these values were 18.18%, 98.39%, 40%, and 95.37%, respectively. In respiratory samples, sensitivity of MTD was found as 83.33%, specificity as 94.05%, positive predictive value as 45.45%, and negative predictive value as 98.95%, whereas in nonrespiratory samples these values were 54.54%, 88.23%, 21.42%, and 97.05%, respectively. Conclusions: In view of the above, the pre-diagnostic EZN test and the MTD method based on nucleic acid amplification should be applied together with the BACTEC 460 system, which is considered as a gold standard, and the evaluation should be made accordingly. Furthermore, MTD should not be used as a screening test due to its high cost, and should rather be preferred in smear-positive samples.