Bronisława Skrzep-Poloczek

Publications of Bronisława Skrzep-Poloczek

• The assessment of lipid peroxidation products and the activity of superoxide dismutase in patients with heart failure.

  Authors: Ewa Romuk, Wojciech Jacheć, Bronisława Skrzep-Poloczek, Celina Wojciechowska, Kamil Kurza, Agnieszka Wróblewska, Marta Cwieczek, Ewa Birkner

  Wiadomości lekarskie (Warsaw, Poland : 1960). 01/2011; 64(2):75-8.

  The study concerns determination of disturbances of the oxidative-antioxidative balance in patients with heart failure. The study group consisted of 37 patients, having the average age of 42.3 +/-The study concerns determination of disturbances of the oxidative-antioxidative balance in patients with heart failure. The study group consisted of 37 patients, having the average age of 42.3 +/- 12.3 years, suffering from idiopathic or inflammatory heart failure. The patients have been qualified as degree II/III in accordance with NYHA scale. A statistically significant increase of anty-oxLDL level (254.28 +/- 80.79 vs 196.64 +/- 83.11 mU/ml; p<0.01) and malondialdehyde level (4.86 +/- 1.74 vs 3.71 +/- 1.19 micromol/l; p<0.05) has been demonstrated after 6-month observation. No statistically significant differences have been found for the activity of total superoxide dismutase (SOD) and its isoenzymes--(SOD-Mn) and (SOD-ZnCu). The results of the study indicate that no changes in SOD activity and increase in lipid peroxidation products level may lead to generation of oxidative stress may play an important role in the pathogenesis of heart failure.

• Lipid peroxidation and vitamin E in human coronary atherosclerotic lesions.

  Authors: Wojciech Jacheć, Andrzej Tomasik, Wojciech Ceglarek, Stanisław Woś, Jan Wodniecki, Celina Wojciechowska, Bronisława Skrzep-Poloczek, Piotr Walichiewicz, Kazimierz Widenka

  Clinica chimica acta; international journal of clinical chemistry. 05/2003; 330(1-2):121-9.

  BACKGROUND: We aimed to assess the oxidant/antioxidant status within the ex vivo human coronary endarterectomy samples. METHODS: To achieve this, we measured products of lipid oxidationBACKGROUND: We aimed to assess the oxidant/antioxidant status within the ex vivo human coronary endarterectomy samples. METHODS: To achieve this, we measured products of lipid oxidation (malondialdehyde, 7-ketocholesterol), lipids (cholesterol, cholesteryl esters) and vitamin E in endarterectomy samples. RESULTS: Content of malondialdehyde in the plaque ranged from 0.23 to 37.36 microg/g. Unesterified cholesterol content ranged from 0.30 to 1.94 mg/g. It was 9.04+/-4.32% of total cholesterol. Total cholesterol content ranged from 1.73 to 23.69 mg/g. Cholesteryl palmitate content ranged from 0.57 to 19.10 mg/g, which is 11.43-60.86% of the total esters (mean+/-SD 40.27+/-18.42%). Cholesteryl oleate content ranged from 0.24 to 5.76 mg/g, being 9.97-21.81% of total esters (mean+/-SD 14.35+/-4.51%). Cholesteryl linoleate content ranged from 1.05 to 8.21 mg/g, being 17.84-45.15% of total esters (mean+/-SD 30.78+/-11.69%). Cholesteryl arachidonate content ranged from 0.51 to 4.20 mg/g, which is 7.56-22.87% of total esters (mean+/-SD 14.60+/-5.60%). The cholesteryl linoleate/cholesteryl oleate ratio (CL/CO) ranged from 1.01 to 4.33. Content of 7-ketocholesterol in the plaque ranged from 0.0 to 577.5 ng/g of wet weight. The 7-ketocholesterol/total cholesterol ratio was 0.003+/-0.003% (range from 0.0% to 0.008%). The 7-ketocholesterol/unesterified cholesterol ratio was 0.024+/-0.023% (range from 0.0% to 0.066%). The plaque content of vitamin E ranged from 0.0 to 40.9 microg/g of wet weight. CONCLUSION: The present study, comprising measurements of lipids, products of lipid peroxidation and vitamin E in 12 human coronary endarterectomy samples, lends the evidence for ongoing lipid peroxidation within an atherosclerotic lesion.

• [The application of cryogenic temperatures in medicine]

  Authors: Ewa Birkner, Aleksander Sieroń, Brygida Beck, Leszek Jagodziński, Ewa Romuk, Bronisława Skrzep-Poloczek, Agata Stanek

  Wiadomości lekarskie (Warsaw, Poland : 1960). 02/2003; 56(1-2):53-6.

  An increased interest in effects of low temperatures on human organisms has been observed during last years. Low temperatures have been applied in cryotherapy and cryosurgery. In our article we showAn increased interest in effects of low temperatures on human organisms has been observed during last years. Low temperatures have been applied in cryotherapy and cryosurgery. In our article we show the influence of low temperatures on different physiological mechanisms and the possibility of their application in the therapy.

• Lipid peroxidation and vitamin E in human coronary atherosclerotic lesions

  Authors: Wojciech Jacheć, Andrzej Tomasik, Wojciech Ceglarek, Stanisław Woś, Jan Wodniecki, Celina Wojciechowska, Bronisława Skrzep-Poloczek, Piotr Walichiewicz, Kazimierz Widenka

  Clinica Chimica Acta.

  Background: We aimed to assess the oxidant/antioxidant status within the ex vivo human coronary endarterectomy samples. Methods: To achieve this, we measured products of lipid oxidationBackground: We aimed to assess the oxidant/antioxidant status within the ex vivo human coronary endarterectomy samples. Methods: To achieve this, we measured products of lipid oxidation (malondialdehyde, 7-ketocholesterol), lipids (cholesterol, cholesteryl esters) and vitamin E in endarterectomy samples. Results: Content of malondialdehyde in the plaque ranged from 0.23 to 37.36 μg/g. Unesterified cholesterol content ranged from 0.30 to 1.94 mg/g. It was 9.04±4.32% of total cholesterol. Total cholesterol content ranged from 1.73 to 23.69 mg/g. Cholesteryl palmitate content ranged from 0.57 to 19.10 mg/g, which is 11.43–60.86% of the total esters (mean±SD 40.27±18.42%). Cholesteryl oleate content ranged from 0.24 to 5.76 mg/g, being 9.97–21.81% of total esters (mean±SD 14.35±4.51%). Cholesteryl linoleate content ranged from 1.05 to 8.21 mg/g, being 17.84–45.15% of total esters (mean±SD 30.78±11.69%). Cholesteryl arachidonate content ranged from 0.51 to 4.20 mg/g, which is 7.56–22.87% of total esters (mean±SD 14.60±5.60%). The cholesteryl linoleate/cholesteryl oleate ratio (CL/CO) ranged from 1.01 to 4.33. Content of 7-ketocholesterol in the plaque ranged from 0.0 to 577.5 ng/g of wet weight. The 7-ketocholesterol/total cholesterol ratio was 0.003±0.003% (range from 0.0% to 0.008%). The 7-ketocholesterol/unesterified cholesterol ratio was 0.024±0.023% (range from 0.0% to 0.066%). The plaque content of vitamin E ranged from 0.0 to 40.9 μg/g of wet weight. Conclusion: The present study, comprising measurements of lipids, products of lipid peroxidation and vitamin E in 12 human coronary endarterectomy samples, lends the evidence for ongoing lipid peroxidation within an atherosclerotic lesion.