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Publications (2)3.65 Total impact

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    ABSTRACT: In this short review a number of recent X-ray diffraction results on the highly ordered striated muscles in insects and in bony fish have been briefly described. What is clear is that this technique applied to muscles which are amenable to rigorous analysis, taken together with related data from other sources (e.g. protein crystallography, biochemistry, mechanics, computer modelling) can provide not only the best descriptions yet available on the myosin head organisations on different myosin filaments in the relaxed state, but can also show the sequence of molecular events that occurs in the contractile cycle, and may also help to explain such phenomena as stretch-activation. X-ray diffraction is clearly an enormously powerful tool in studies of muscle. It has already provided a wealth of detail on muscle ultrastructure; it is providing ever more fascinating insights into molecular events in the 50-year old sliding filament mechanism, and there remains a great deal more potential that is as yet untapped.
    Advances in experimental medicine and biology 02/2005; 565:45-60; discussion 359-69. · 1.83 Impact Factor
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    ABSTRACT: The muscle crossbridge power stroke on actin appears to involve a change in angle between the actin-attached motor domain and the neck region of the myosin heads (the ‘tilting neck hypothesis’). However, this mechanism has not been proved beyond doubt and a reasonable question to ask is how actual proof might be achieved. This is essentially a structural question. The question can be put as ‘what are the molecular shapes that the myosin head adopts during the crossbridge cycle on actin?’. A further question that also needs answering is ‘how do the biochemical stages of the actin-myosin ATPase cycle map onto the structural changes that are seen?’. The purpose of the present paper is to address how the first question might be answered and to start to make suggestions about the second.
    Advances in experimental medicine and biology 02/2003; 538:251-66; discussion 266. · 1.83 Impact Factor