[show abstract][hide abstract] ABSTRACT: Combining the technique of multiplex-PCR and the fluorescent semi-automated detection, a large-scale genome scanning was performed for 440 chickens, which was derived from China Agricultural University chicken resource families, within three generations. Fifty-five microsatellite markers were analyzed for this study. Those 55 microsatellite loci accorded with the characters of Mendelian co-inheritance. The heterozygosities ranged from zero to 0.89, with 72% of loci having a heterozygosity of more than 0.60. The polymorphism information content (PIC) ranged from 0 to 0.85, in which 70% of those loci had a PIC of more than 0.50 but their distribution varied in line A and line C. The allele frequency was significantly different between line A and line C at most loci (P < 0.01). At the same time, gene accordance inclination was found in line C. The Nei population resemble coefficient and standard genetic distance were 0.1002 and 0.8928.
[show abstract][hide abstract] ABSTRACT: Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation, especially applied in the genetic trait modification of transgenic animal. Our paper reviewed the history of transgenic animal, somatic cloning and gene targeting, which indicates the influence towards the current status and prospect of the transgenic animal production.
[show abstract][hide abstract] ABSTRACT: In the research,the outputs of different cycle parameters, PCR buffer and reaction volumes are compared. The results indicated that the annealing temperature, annealing time, elongated time and ingredient of PCR buffer affected mutiplex PCR, but the reaction volume and cycle number had few effect on it.