Jianming Huang

Fudan University, Shanghai, Shanghai Shi, China

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Publications (6)7.86 Total impact

  • Jianming Huang, Ni Li, Yunqiu Yu, Weiyu Weng, Xubing Huang
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    ABSTRACT: A specific, precise and accurate high-performance liquid chromatography (HPLC) method for the determination of aglycone conjugated metabolites of scutellarin in plasma after enzymolysis to scutellarein (the aglycone of scutellarin) was developed and validated. The chromatographic separation was performed on a Lunar C18(2) reversed-phase column at a column temperature of 40 degrees C. The mobile phase, delivered at 1.0 ml/min, consisted of acetonitrile-KH2PO4 buffer (40 mM, pH 2.5) (33:67, v/v). The detection wavelength was set at 335 nm. Scutellarein and I.S. (quercetin) were isolated by a liquid-liquid extraction after incubating the plasma samples with beta-glucuronidase/sulfatase. The method was validated using scutellarin spiked plasma as standards. Linearity was confirmed in the concentration range of 0.2165-4.329 nmol/ml, R.S.D.s were within 8.32%, and the recoveries of scutellarein ranged from 101.2 to 108.6%. The method is applicable to the pharmacokinetic study of aglycone conjugated metabolites of scutellarin in rats after oral administration of scutellarin.
    Journal of Pharmaceutical and Biomedical Analysis 03/2006; 40(2):465-71. · 2.95 Impact Factor
  • Ni Li, Jianming Huang, Weiyu Weng, Shengmin Su, Yunqiu Yu
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    ABSTRACT: An HPLC method was established for preparation of scutellarein from scutellarin using an enzymolysis reaction. The aglycone was identified as scutellarein with its chromatogram, UV, LC‐MS, and H‐NMR spectra. Acid hydrolysis was tried in preliminary experiments, but enzymolysis was adopted after optimization of reaction conditions. High purity scutellarein (content 98.04%∼98.36%) was prepared in this way for the first time, which could be used as a working reference substance.
    Journal of Liquid Chromatography & Related Technologies - J LIQ CHROMATOGR RELAT TECHNO. 01/2006; 29(9):1297-1305.
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    ABSTRACT: A rapid and specific reversed-phase high performance liquid chromatography (RP-HPLC) method for the determination of palmatine in rabbit plasma has been developed and validated. The chromatographic separation was performed on a C18 column at 40 °C. The mobile phase, delivered at 1.0 mL min−1, consisted of acetonitrile/phosphate buffer (pH 3.0) 40:60 (v/v). The detection wavelength was set at 345 nm. Palmatine and internal standard (IS) berberine were extracted from plasma by solid-phase extraction using C18 cartridges. Linearity was confirmed in the concentration range of 0.01 to 5 μg mL−1, the inter-day and intra-day RSDs were within 10.0, the recoveries of palmatine ranged from 93.1 to 110.3, and the limit of detection (LOD, S/N > 3) was 0.002 μg mL−1. The method is applicable to the determination of palmatine in rabbit plasma after intravenous administration of palmatine.
    Chromatographia 10/2005; 62(9):471-474. · 1.44 Impact Factor
  • Annali di Chimica 07/2005; 95(6):473-7. · 0.99 Impact Factor
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    ABSTRACT: A high performance liquid chromatographic method was developed for the determination of ligustrazine in human plasma. The chromatographic separation was performed on a Luna C18 column (150 mm x 4.6 mm i.d., 5 microm) at column temperature of 40 degrees C. The mobile phase, a mixture of methanol-acetonitrile-acetate buffer of pH 5.0 (50:8:42, v/v), was delivered at a flow rate of 1.0 mL/min. The detection wavelength was 280 nm. Plasma samples were prepared with a C8 solid-phase extraction column. Linearity was confirmed in the mass concentration range of 25-5000 microg/L with the correlation coefficient of 0.9999. The extraction recovery of ligustrazine ranged from 96.72% to 100.90%. The relative standard deviations (RSDs) of intra- and inter-day assay at the mass concentrations of 50, 500 and 3000 microg/L were less than 8.64% and the accuracies were between 99.59%-103.26%. The limit of detection (LOD) was 10 microg/L. The results of this method validation satisfactorily meet the acceptance criteria of bioanalysis and the method is applicable to the pharmacokinetic studies of ligustrazine in human beings.
    Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 06/2005; 23(3):276-8.
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    ABSTRACT: A sensitive and specific high-performance liquid chromatography (HPLC)-electrospray ionization tandem mass spectrometry (MS-MS) was developed for the determination of bulleyaconitine A (BLA) in human plasma. BLA and internal standard (I.S.) ketoconazole were extracted from the plasma by a liquid-liquid extraction. The supernatant was evaporated to complete dryness and reconstituted with acetonitrile containing 0.1% acetic acid before injecting into an ODS MS column. The gradient mobile phase was composed of a mixture of acetonitrile (containing 0.1% acetic acid, v/v) and 0.1% acetic acid aqueous solution eluted at 0.3 ml/min. BLA and I.S. were determined by multiple reaction monitoring using precursor-->product ion combinations at m/z 644.6-->584.3 and 531.2-->81.6, respectively. Linearity was established for the concentration range of 0.12-6 ng/ml. The recoveries of BLA ranged from 96.93 to 113.9% and the R.S.D. was within 20%. The method is rapid and applicable to the pharmacokinetic studies of BLA in human.
    Journal of Chromatography B 03/2005; 816(1-2):315-20. · 2.49 Impact Factor