[Show abstract][Hide abstract] ABSTRACT: Piroplasms are tick-transmitted, intracellular, hemoprotozoan parasites that cause anorexia, fever, anemia, and icterus. Theileriosis is caused by Theileria sergenti and causes major economic losses in grazing cattle in Japan and Korea. In May 2003, we examined the antigenic diversity of the major piroplasm surface protein (MPSP) gene in 35 healthy Jeju black cattle that were born and raised at the National Institute of Subtropical Agriculture. On microscopic examination of Giemsa-stained blood smears, 9 of 35 cattle had intra-erythrocytic piroplasms. Hematological data were within normal range for all 35 cattle. Amplification of DNA from all blood samples using universal MPSP gene primers showed mixed infections with C, I, and B type Theileria spp. Type C was identified in 20 of 35 blood samples, and type B was identified in 17 samples. Allelic variation was seen in type B.
Journal of Veterinary Science 07/2008; 9(2):155-60. · 0.93 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Hemodialysis (HD) has sufficient efficacy as a conventional diffusive treatment for removing small molecules, whereas hemofiltration (HF), which is a convective treatment, has an improved the clearance of intermediate-sized molecules. This paper reports a combined treatment (CT) which combines the diffusive and convective efficacies of HF several times weekly with HD weekly. CT modalities with various schedules and prescriptions are described mathematically using a variable-volume two-compartment kinetics model, and the kinetic parameters were obtained from previous clinical reports and a hemodialysis-related database. The blood concentration profiles of the three waste molecules for 52 weeks were calculated in order to compare the capability of removing small and intermediate-sized molecules to those of other renal treatments. The results by a computer simulation show that CT can reduce the frequency of sessions and the volume of replacement fluid compared with daily convective treatment, and achieve the adequate treatment efficiency with both small and intermediate-sized molecules for chronic renal failure patients.
Computer Methods and Programs in Biomedicine 04/2008; 89(3):275-81. · 1.56 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The study was carried out to investigate the genetic polymorphism of the serum proteins of horses in Cheju. They were assigned to three groups; 45 Cheju native horses(CNH), 60 Cheju racing horses(CRH) and 60 Thoroughbreds(TB). We analyzed the phenotypes and gene frequencies of serum proteins which were albumin (Alb), vitamin-D binding protein(GC), esterase (ES), A1B glycoprotein(A1B) and transferrin(TF) loci using horizontal polyacrylamide gel electrophoresis (HPAGE). All of the loci, except A1B in TB, showed polymorphisms and different allelic and phenotypic frequencies in all three groups. ESS and TFF1 were not observed in CNH. Allelic frequencies of AlbB, ESI, TFD and TFF1 were high in TB. All of the loci, except ES locus in CRH, appeared to be in a state of Hardy-Weinberg equilibrium from goodness-of-fit test in all three groups. Heterozygosity estimates at Alb, ES and TF loci were high, but GC and A1B loci were low in all three groups. Average heterozygosities in CNH, CRH and TB were 0.3535, 0.3555 and 0.2726, respectively. Results showed differences in the frequencies of alleles and phenotypes of several serum protein loci between CNH and CRH, suggested that CRH might be crossed with other breeds of horses in some degree.
Journal of Veterinary Science 01/2003; 3(4):255-63. · 0.93 Impact Factor