[Show abstract][Hide abstract] ABSTRACT: Pertaining to reported high prevalence of brucellosis in Pakistan, district Layyah on
account of having thick sheep population was selected for this study. A total of 384
sheep blood samples were collected randomly from different selected private herds
in the district, and tested through Modified Rose Bengal (mRB) test and Indirect
Enzyme Linked Immunosorbent Assay (iELISA) for the serological analysis against
the Brucella antibodies. Positive samples from these two tests were further
subjected to Competitive Enzyme Linked Immunosorbent Assay (cELISA). The
individual based seroprevalence of brucellosis in sheep was found to be 7.0% by
mRB. Herd based prevalence was 42.5%. The highest (P<0.05) seroprevalence
(16.8%) was observed in Tehsil Layyah as compared to Tehsils Karor (2.3%) and
Choubara (4.5%). Non-significant differences were recorded between breeds, age and
sex groups and also for the animals with or without history of abortion. In case of
indirect and competitive ELISA, no sheep serum sample was found to be positive.
[Show abstract][Hide abstract] ABSTRACT: The current study was conducted to determine the level of some major biochemical constituents in seminal plasma of Lohi rams, and its correlation with semen physical characteristic. For this purpose six mature healthy rams were selected from the sheep herd maintained at Livestock Production and Research Institute, Bahadurnagar, Okara. All the experimental rams were kept under similar feeding and management conditions. Total 48 semen samples were collected during the breeding season (mid-autumn). Immediately after the collection samples were subjected to quantitative and qualitative sperm parameters. Seminal plasma was separated by centrifugation at 3000 × g for 20 min and samples were stored at −20 °C till the analysis. The mean concentration of Na, K, Mg, Cu, P, Zn, and total protein (TP) in seminal plasma was 222.90 mg/dl, 48.21 ppm, 10.77 mg/dl, 2.22 mg/dl, 10.26 mg/dl, 0.35 ppm and 1.87 mg/dl respectively. Statistical analysis of the data showed the significant negative correlation between biochemical constituent (Na, Mg, Cu) and sperm characteristics (ejaculated volume, mass activity, motility percentage, sperm concentration and spermatozoa abnormalities). There was significantly positive correlation between biochemical constituent (Zn, TP, P) and sperm characteristics (ejaculated volume, mass activity, motility percentage and sperm concentration). However they were negatively correlated with sperm abnormalities.
Small Ruminant Research 06/2013; 113(1):175–178. · 1.10 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The present study was conducted during 2005 and 2006 on 200 Nili-Ravi buffaloes kept in two agroecological zones (irrigated [zone 1] and rain-fed [zone-2]) of Punjab, Pakistan, with the objective to determine the level of trace minerals (Cu, Fe, Zn, Se) in serum of the buffaloes suffering from vaginal prolapse and to compare them with their healthy counterparts. In each zone 50 buffaloes suffering from prepartum vaginal prolapse during their seventh month of gestation were identified through survey. Vaginal prolapse-affected buffaloes belonging to zone 1 were identified as group VPB1 (N = 50), whereas buffaloes belonging to zone 2 were recognized as VPB2 (N = 50). The buffaloes of control group in zone 1 and zone 2 were identified as NCB1 and NCB2, respectively. The blood samples in all four groups of buffaloes were collected three times, i.e., first when these animals were in the eighth month of gestation, second during the eighth to ninth month of gestation, and finally when these animals were in the ninth or later month of gestation. The mean serum copper concentrations in buffaloes of group VPB1 were significantly lower (P < 0.05) in comparison with NCB1 and NCB2, whereas there were nonsignificant differences (P > 0.05) in copper concentrations between VPB1 and VPB2. There was a significant difference (P < 0.05) of iron concentration in VPB1 compared with NCB1 and NCB2. Similarly, VPB2 also had significantly lower (P < 0.05) iron concentrations compared with NCB1 and NCB2. Serum zinc concentrations were significantly lower (P < 0.05) in animals of the VPB1 group when compared with NCB1 and NCB2. Similarly, lower zinc concentrations were observed in VPB2 in comparison with NCB1 and NCB2. There was significantly lower (P < 0.05) zinc concentration in affected buffaloes (VPB1 and VPB2) from the ninth month of gestation to term when compared with those in the eighth to ninth mo of gestation, and with those not yet in the eighth month of gestation. Serum selenium concentration were significantly higher (P < 0.05) in control group buffaloes (NCB1 and NCB2) in comparison with vaginal prolapse-affected buffaloes (VPB1 and VPB2). During different stages of gestation, mean serum selenium concentrations varied nonsignificantly (P > 0.05) within each group of buffalo. Based on information obtained from this study, it was concluded that the low serum concentration of copper and selenium are linked to increased incidence of vaginal prolapse in buffaloes during the last trimester of gestation.
[Show abstract][Hide abstract] ABSTRACT: The present study was planned to evaluate the efficacy of vitamin E-selenium administration during last month of gestation in improving the productive and reproductive performance of dairy buffaloes and on growth performance of their calves. For this purpose, 40 Nili-Ravi buffaloes in their last month of pregnancy were randomly divided into two groups. The buffaloes in group 1 were treated with 10 ml of vitamin E-Se (Farvet Holland), containing 50 mg of vitamin E and 0.5 mg of sodium selenite/ml) intramuscularly on day 30 and 15 prior to expected date of parturition, while buffaloes in group II served as control. All the experimental buffaloes were kept under similar management and feeding conditions. The data on productive performance showed that the colostrum production was 22% higher (P<0.05) in treated buffaloes (14.15 3.29L) as compared to their counterparts (11.51 3.39L). The average milk production of first 90 days of lactation was 7% higher (P>0.05) in Vit E-Se treated buffaloes (706.90 314.29L) as compared to control group (663.85 225.46L). Data on reproductive performance showed that vitamin E-Se administration significantly (P<0.01) improved the conception rate (66 vs 45%) and estrus rate (90 vs 55%). The mean calf body weight at birth and at 4 weeks of age was higher by 3 and 12%, respectively, in calves born to vitamin E-Se treated buffaloes, the difference was non significant (P>0.05). It was inferred that the administration of vitamin E-Se during late gestation in buffaloes have beneficial effect on colostrum production, post partum estrus rates and conception rates.
[Show abstract][Hide abstract] ABSTRACT: The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates) from each bull were collected. Each semen sample was divided into two parts. One part was used for the evaluation of semen by conventional method, while the other part was subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a significant (P<0.05) positive correlation between progressive motility, morphologically normal spermatozoa, sperm viability and percentage of HOS test positive spermatozoa for both species. It was inferred that HOS test could be a valuable method for routine evaluation of semen for artificial insemination.
[Show abstract][Hide abstract] ABSTRACT: Jamil H., H.A. Samad, N.U. Rehman, Z.I. Qureshi, L.A. Lodhi: In vitro Maturation and Fertilization of Riverine Buffalo Follicular Oocytes in Media Supplemented with Oestrus Buffalo Serum and Hormones. Acta Vet. Brno 2007, 76: 399-404. Effects of two maturation media (TCM-199 and Ham's F-12) with and without the addition of oestrus buffalo serum (OBS) and hormones (FSH, LH, E2) on the maturation rate of buffalo follicular oocytes were evaluated. The results revealed a signifi cant (P < 0.05) increase in the maturation rate when the OBS and hormones were added to TCM-199 than in Ham's F-12 medium. The percentage of maturation rates in TCM-199 + hormones + OBS, TCM-199 + hormones, TCM-199 + OBS and TCM-199 were 77.44, 55.17, 62.28 and 26.62 percent, respectively. While in Ham's F-12 + OBS + hormone, Ham'F-12 + hormone, Ham's F-12 + OBS and Ham's F- 12 were 32.85, 27.52, 31.38 and 13.46 percent, respectively. A signifi cantly higher (P < 0.05) fertilization rate was recorded for modifi ed Ca 2+ free Tyrode's medium (63.72%) than in TALP (10.9%) and IVF-TL (32.18%). Thus, TCM-199 containing hormones and OBS appeared better for in vitro maturation, whereas modifi ed Ca 2+ free tyrode's medium was found to be more suitable for in vitro fertilization of buffalo follicular oocytes. Reproduction, Bubalus bubalis, TCM-199, Ham's F-12, FSH, LH, E2 Riverine buffaloes (Bubalus bubalis) are hardy dairy animals, resistant to climate, stress and diseases. However, problems like delayed onset of reproductive maturity, seasonality of breeding, long calving interval, latent oestrus, low number of primordial follicles and
Acta Veterinaria Brno 01/2007; 76(3):399-404. · 0.45 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The present study was designed to compare fertilization rates following oocyte exposure to spermatozoa from different buffalo bulls, using three sperm preparation methods i.e. percoll density gradient, swim-up (modified Ca2 free Tyrode’s medium and TALP medium) and sodium citrate washing prior to co-incubation with oocytes. Buffalo oocytes were aspirated from ovarian follicles within 1.5 to 2 hours after slaughter. They were matured in TCM-199 supplemented with 20% oestrus buffalo serum drops under mineral oil in CO2 incubator at 39C for 24 hours. Matured oocytes were transferred to the fertilization droplets and inseminated with 1x106 capacitated sperms prepared by different experimental methods. Data collected on recovered sperm motility immediately after treatment and 24 hours after incubation (at 37C) and cleavage rate of co-incubated oocytes were subjected to statistical analysis. The percentage of motile spermatozoa was significantly higher (P<0.05) in semen samples prepared by swim-up method in Ca2 free Tyrode’s medium compared to other experimental techniques. Bull wise comparison showed significantly higher (P<0.05) motility in bull B1 (50.50 ± 5.92%), followed by bull B2 (46.46 ± 5.99%) and B3 (45.96 ± 5.79%). Fertilization rate was also significantly (P<0.05) higher for spermatozoa prepared by Swim-up method in Ca2 free Tyrode’s medium (63.75 ± 2.81%), followed by sodium citrate (26.70 ± 5.08%), swim-up TALP (29.14 ± 3.74%) and Percoll gradient density (23.89 ± 3.88%). Fertilization rate was significantly higher (P<0.05) in oocytes inseminated with semen from bull B1 (43.43 ± 8.59%), followed by B2 (33.38 ± 9.95%) and B3 (30.80 ± 9.56%). The results of present study indicate that bulls and sperm preparation methods differ in their contribution to in vitro fertilization rate. Further studies are suggested to ascertain the factors responsible for such specific effects.
[Show abstract][Hide abstract] ABSTRACT: Serum glucose, total proteins, calcium and inorganic phosphorus were determined in estrual, pregnant, infertile and regular breeder mares, each group comprising of 10 animals. The mares were grouped on the basis of rectal examination, history and records. There was a significant (P<0.05) difference in the serum glucose levels between the pregnant and estrual mares, pregnant and regular breeder mares, infertile and regular breeder mares and between infertile and estrual mares. The highest values of serum glucose were recorded in regular breeder mares, whereas the lowest values were recorded in infertile mares. The highest values for total proteins were recorded in regular breeder mares and the lowest in pregnant mares, difference being significant (P<0.05) between the pregnant and estrual and pregnant and regular breeder mares. The highest values of serum calcium were recorded in estrual mares and lowest for regular breeder mares. There was a significant (P<0.05) difference between the estrual and pregnant mares, estrual and infertile mares and between estrual and regular breeder mares. The highest values of serum inorganic phosphorus were recorded for regular breeder mares whereas the lowest values were recorded in pregnant mares. The analysis of data indicated non significant (P>0.05) difference in the serum phosphorus levels among all the groups of mares.
[Show abstract][Hide abstract] ABSTRACT: A total of 32 Sahiwal cows in the last trimester of pregnancy were used to determine the effect of different immunopotentiators on their postpartum reproductive performance. The cows were divided into four groups. Group-1 served as untreated control, while groups 2, 3 and 4 were treated with levamisole hydrochloride (0.5 mg/Kg body weight orally), vitamin E-selenium (10 ml intramuscularly per cow) and BCG vaccine (0.5 ml subcutaneously per cow), respectively. These immunopotentiators were given twice with one week interval at about 60 days prior to expected date of calving. Various reproductive parameters during subsequent postpartum period, i.e., placental expulsion period (hours), uterine involution period (days), postpartum estrus interval (days), number of services per conception and service period (days) were studied. It was noted that duration of all these parameters (mean ± SE) were longest in cows of untreated control group (10.56 ± 0.57, 52.38 ± 1.38, 125.38 ± 25.79, 2.63 ± 0.50 and 286.88 ± 39.04, respectively) and shortest in the cows of vitamin-E selenium treated group (8.88 ± 0.30, 31.13 ± 0.52, 70.00 ± 15.92, 1.50 ± 0.19 and 115.00 ± 19.75, respectively). Two reproductive parameters, i.e., postpartum estrus interval and number of services per conception were non-significantly lesser in the cows of treated groups as compared to those of untreated control. Three reproductive parameters, i.e., placental expulsion period, uterine involution period and service period were reduced significantly (p<0,05) in vitamin-E selenium and BCG treated groups as compared to control. The cows of levamisole hydrochloride treated group showed significant (p<0.05) reduction only in uterine involution period as compared to untreated control. All the treated groups differed non-significantly amongst each other for all reproductive parameters except uterine involution period.
[Show abstract][Hide abstract] ABSTRACT: In the present study, the effects of gossypol on semen characteristics in Teddy male goats were studied. Nine Teddy male goats were randomly divided into three equal groups named A, B and C. Animals in all groups were fed concentrated ration without cottonseed cakes (CSC) at the rate of 3% of their liveweight for a period of 30 days and it was named as pre-treatment period. Just after the completion of this period, animals in group A were fed control ration (without gossypol), those in group B were fed ration which contained unboiled CSC as a source of free and bound gossypol, while animals in group C were given ration containing CSC boiled at 100C for 1 hour as a source of bound gossypol These experimental rations were fed to animals of respective groups at the rate of 3% of their liveweight for a period of 90 days and it was named as treatment period. Feeding of ration containing gossypol to Teddy male goats did not affect the colour, volume, mass activity, sperm concentration, percentage of dead spermatozoa, liveability and absolute index of liveability of spermatozoa at 37°C. However, it affected significantly (P<0.05) the pH, per cent motility of spermatozoa and percentage of morphologically abnormal spermatozoa. The Teddy male goats fed rations containing a combination of free and bound gossypol showed a significant (P<0.05) increase in the pH and a decrease in motility of spermatozoa which was statistically lower than those fed control diet or diet containing bound gossypol. It was concluded that rations containing a combination of free and bound gossypol (unboiled CSC) or bound gossypol only (boiled CSC) adversely affected the semen quality of Teddy male goats in terms of sperm motility and morphologically abnormal spermatozoa in ejaculates.
[Show abstract][Hide abstract] ABSTRACT: A case of acute puerperal metritis in a dromedary camel developing in the wake of dystokia corrected under unhygienic condition is described. The condition was associated with fever, foul-smelling purulent uterine discharge and pasty faeces. Pretreatment hematological examination indicated leukocytosis (22.05 x 103/mm3), owing to monocytosis (24 %). Microbiological examination of uterine discharge revealed a mixed bacterial infection with E. coli. Bacillus and streptococci which were all sensitive to nortloxacin, gentamicin, and amoxycillin, Faecal examination indicated a mixed infection with nematodes, Intrauterine administration of oxytetracycline with parenteral administration of amoxycillin, dipyrone and oral administration of oxfendazole successfully treated the case. The Principles of treatment of acute puerperal metritis have been discussed.
[Show abstract][Hide abstract] ABSTRACT: The colostral and seral antibody titre was significantly higher in leavamisole treated group of buffaloes. Other immunopotentiator group differed non significantly.Levamisole hydrochloride can be used effectively along with vaccine in pregnant buffaloes to produce colostral specific antibodies.