Marie-Elisabeth Sarciron

Claude Bernard University Lyon 1, Villeurbanne, Rhône-Alpes, France

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Publications (13)27.74 Total impact

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    ABSTRACT: Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium. In each country, five dogs were vaccinated with the associated EgA31 and EgTrp; three dogs received only the vector Salmonella; and six dogs were used as different controls. The vaccinated dogs received two oral doses of the vaccine 21 d apart, and were challenged 20 d later with 75,000 living protoscoleces. The controls were challenged under the same conditions. All dogs were sacrificed 26–29 d postchallenge, before the appearance of eggs, for safety reasons. We studied the histological responses to both the vaccine and control at the level of the duodenum, the natural localization of the cestode. Here we show a significant decrease of parasite burden in vaccinated dogs (70% to 80%) and a slower development rate in all remaining worms. The Salmonella vaccine EgA31-EgTrp demonstrated a high efficacy against E. granulosus promoting its potential role in reducing transmission to humans and animals. Copyright: ß 2008 Petavy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by INCO-MED (ICFP599A3PR01) 2000-2004. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist.
  • Samia Lahmar, Marie-Elisabeth Sarciron, Mondher Rouiss, Mohamed Mensi
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    ABSTRACT: Assessing the baseline prevalence and the re-infection rate of E. granulosus and other cestodes in dogs is important for a control program based on regular dog dosing treatment with praziquantel mainly to young rural dogs. Three hundred and seventy five rural and semi-stray dogs from Jendouba (207 dogs) and Béjà (168 dogs) Departments in Northern-West of Tunisia, were examined to evaluate both the baseline prevalence of intestinal helminths and re-infection rates with Echinococcus granulosus and other cestodes. Parasites were collected in faeces following arecoline hydrobromide purge at the first examination of the dogs and at 4 intervals of reexamination: 2, 4, 8 and 12 months. After each examination, dogs were treated with praziquantel. The global baseline prevalence with Intestinal helminths in dogs was about 35 %. E. granulosus and other helminths were found in 3.5% (E. granulosus); 9.55% (Taenia hydatigena); 6.36% (Taenia pisiformis); 4.77% (Taenia multiceps); 8.59% (Dipylidium caninum), 5.41% (Mesocestoïdes sp.) and Ankylostoma caninum (13.37%). Dogs were re-infected with E. granulosus, T. hydatigena, T. pisiformis and D. caninum, 2 months after the arecoline hydrobromide purge while T. multiceps and Mesocestoïdes sp. infection reappeared 4 months later. A control program against Cystic/Echinococcosis, based on a regular treatment of the dog population with praziquantel every 60 days seems necessary. However in practice a six-monthly treatment during ten years would must to decrease the infection pressure of E. granulosus and cease transmission for human.
    La Tunisie médicale 08/2008; 86(7):657-64.
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    ABSTRACT: The success of the chemotherapeutic treatment of hydatid disease is based upon the drug ability to operate on the germinal layer and on the protoscolices of the hydatid cyst interior at adequate concentrations for sufficient periods. The goal of this study was to evaluate the ability of the drug diffusion through the cyst membrane from sheep hydatid cysts and the increase of drug concentration in the cyst environment. In the first part of this work, the permeation behaviour through the hydatid cyst membrane was studied with five model molecules, having different molecular descriptors (logP, molecular weight, polar surface area ...) onto static Franz glass diffusion cells. A good correlation has been observed between the permeation coefficient and the partition coefficient, log P (r=0.951). In the second part, albendazole-loaded nanoparticles (about 300 nm) prepared by the emulsion solvent evaporation method have shown a sufficient entrapment efficiency (36.4 +/- 6.4%) to raise the apparent solubility of albendazole. The diffusion of drug from the nanoparticles across the hydatid cyst membrane was also improved compare to albendazole suspension. These results have shown the interest of the albendazole-loaded nanoparticles for the treatment of hydatid cysts in the future.
    International Journal of Pharmaceutics 05/2008; 353(1-2):223-32. · 3.99 Impact Factor
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    ABSTRACT: Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium.In each country, five dogs were vaccinated with the associated EgA31 and EgTrp; three dogs received only the vector Salmonella; and six dogs were used as different controls. The vaccinated dogs received two oral doses of the vaccine 21 d apart, and were challenged 20 d later with 75,000 living protoscoleces. The controls were challenged under the same conditions. All dogs were sacrificed 26-29 d postchallenge, before the appearance of eggs, for safety reasons.We studied the histological responses to both the vaccine and control at the level of the duodenum, the natural localization of the cestode. Here we show a significant decrease of parasite burden in vaccinated dogs (70% to 80%) and a slower development rate in all remaining worms. The Salmonella vaccine EgA31-EgTrp demonstrated a high efficacy against E. granulosus promoting its potential role in reducing transmission to humans and animals.
    PLoS Neglected Tropical Diseases 02/2008; 2(1):e125. · 4.57 Impact Factor
  • Arnaud Ghérardi, Marie-Elisabeth Sarciron
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    ABSTRACT: The need of intracellular parasites to retrieve nutrients and fulfill their energy requirements is achieved by manipulating the host's metabolism. With the spread of AIDS, research on purine metabolism has gained in importance with the aim to develop drugs against opportunistic infections. Many studies over the past ten years have yielded contradictory results, but this review tries to clarify these findings by exposing the latest data concerning purine transport and the specific activities of the major enzymes of the purine salvage pathway of Toxoplasma gondii, Plasmodium falciparum and Cryptosporidium parvum.
    Trends in Parasitology 09/2007; 23(8):384-9. · 5.51 Impact Factor
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    ABSTRACT: The main of this study was to show the rapidity of the protoscolicide action of a synthetic compound, dipeptide methyl ester when it is injected under echographic control into hydatid cyst of sheep. Fourthty sheep with hydatid cysts, repaired at echography and punctionable are treated by dipeptide methyl ester injection at the dose of 110 mM. In vitro tests have allowed to define the efficacy dose of dipeptide methyl ester which is 110 mM. At echography, after injection of the drug, from the first minutes, a detachment of the inner membrane, a diminution of the size of the treated cyst were observed. The cyst content is modified. The sheep autopsy was realized after 4.6.12 and 17 weeks after the injection and showed a size reduction, a treated cyst calcification. The dipeptide methyl ester injection into hydatid cyst induces rapidly a morphological alteration, they are calcified. The advantage of this compound is its very rapidity action, this could decrease dissemination risks of hydatid liquid in the organism during operation. Also, this drug permits to reduce the operation time.
    La Tunisie médicale 10/2006; 84(9):563-8.
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    ABSTRACT: 3-Arylamino-2-phenylsulfinylnaphthoquinones, 2,3-diarylthio-naphthoquinones and 2-phenylsulfinyl-3-arylthio-1,4-dihydronaphtalenes are synthesized and tested against five fungi. The activities of these products were better than amphotericine B against all the strains except for Candida albicans.
    European Journal of Medicinal Chemistry 07/2006; 41(6):773-8. · 3.50 Impact Factor
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    ABSTRACT: The in vitro cultivation of Cryptosporidium parvum in the non-adherent cell line THP-1 was evaluated for its capability as a useful additional model to investigate the effect of drugs on this parasite. The purine analog antiviral 2′,3′-dideoxyinosine (ddI) was evaluated and compared to the reference molecule paromomycin in sequential 24 hour experiments beginning at 24 and 72 hour post-infection. The ability of this technique to evaluate the various parasite stages showed that ddI displayed a dose-dependent efficacy especially on the trophozoite and sexual stages. Paromomycin displayed a lower efficacy than previously reported. Both drugs induced a decrease in the number of multiparasitized cells. These results indicate that the purine salvage pathway should be a key chemotherapeutic target against C. parvum.
    FEMS Microbiology Letters 01/2006; 176(1):235 - 240. · 2.05 Impact Factor
  • Arnaud Gherardi, Simone Peyrol, Marie-Elisabeth Sarciron
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    ABSTRACT: Purine nucleoside phosphorylase (PNP, EC 2.4.2.1) activity was revealed by enzyme histochemistry in Toxoplasma gondii ME49 strain isolated from murine cerebral cysts and from in vitro cultivation. The activity of the enzyme was revealed by an insoluble electron-opaque precipitate of lead phosphate at the site of the reaction. In bradyzoites and tachyzoites of T. gondii, the enzyme activity could be observed only in the cytoplasm. In bradyzoites, one or two foci of important PNP activity were detected near the nucleus. In tachyzoites, an important PNP activity underlined the plasma membrane. For both bradyzoites and tachyzoites, localization neither in the nucleus nor in cytoplasmic organelles could be detected.
    Medical Molecular Morphology 01/2006; 38(4):251-5. · 1.17 Impact Factor
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    ABSTRACT: In a previous work, we have showed in mice infected with an avirulent strain of Toxoplasma gondii and receiving a didanosine treatment, an important decrease of brain cysts. It is why, the purpose of this study was to investigate the effect of didanosine treatment on AIDS patients having developed Toxoplasma encephalitis. 60 patient reports were analyzed: 22 patients (group 1) did not received didanosine in their antiretroviral treatment and 38 (group 2) were treated with didanosine. The results showed that an antiretroviral therapy was prescribed for 93% of patients, 50% of them received only zidovudine and protease inhibitors were prescribed for 37%. The regimens given most frequently were those including zidovudine plus lamivudine or zidovudine plus indinavir. Among the group 1, 18% have had a relapse of Toxoplasma encephalitis. In the group 2, 37% of the patients suffered from one episode of TE while 16% have had two TE after the pause in their didanosine treatment, the maximum occurring between 4 and 24 months after the pause of didanosine. This study showed that didanosine seems to have an effect on cerebral cysts. Also, this work made a synthesis about the different treatment used in AIDS patients and the new molecules yet in development against T. gondii.
    Current HIV Research 11/2004; 2(4):301-7. · 2.03 Impact Factor
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    ABSTRACT: Quinonic derivatives were tested against a virulent RH strain of Toxoplasma gondii maintained in cell culture in THP-1, a human myelomonocytic cell line. The derivatives were tested at various doses (0.5-4 microg/ml) and compared with the reference molecules clindamycine, sulfadiazine, pyrimethamine and atovaquone. The percentage of parasite growth inhibition was observed after 72 h of incubation. The tested derivatives are bicyclic, tricyclic or tetracyclic quinones. Eight of these compounds exhibit over 70% inhibition of parasite growth; and two were nearly equipotent to pyrimethamine. These data indicate that the most active compounds against the RH strain of T. gondii are bis-heterocyclic quinones.
    Parasitology Research 12/2002; 88(11):969-71. · 2.85 Impact Factor
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    Philippe Lawton, Marie-Elisabeth Sarciron, Anne-Françoise Petavy
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    ABSTRACT: The alkaline phosphatases (EC 3.1.3.1) from Echinococcus granulosus and E. multilocularis (Cestoda) were compared to each other and to a liver-type enzyme. The purified proteins (210 and 220 kDa, respectively) had a tetrameric structure composed of 4, 56/53 kDa subunits. Enzymatic removal of their N-linked sugar moieties abolished the differences in their apparent molecular weight under reducing conditions. After phase separation in Triton X-114, the E. multilocularis enzyme was the most amphiphilic, and treatment with PI-PIC reduced the amount of the parasite alkaline phosphatases that were in a hydrophobic form by about 50%. Both parasite enzymes were highly resistant to heat denaturation and insensitive to the inhibitors l-phenylalanine and l-leucine. In addition, l-homoarginine, levamisole and ZnCl2 can be used to differentiate the parasite and mammalian liver-type enzymes from each other. The Echinococcus alkaline phosphatases have original biochemical properties when compared to the mammalian liver-type enzyme.
    Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology 11/1995; · 2.07 Impact Factor
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    ABSTRACT: The biological effects of Phe-Phe-OMe (1) were evaluated in gerbils against the causative agent of alveolar echinococcosis, Echinococcus multilocularis. Morphological damage to the parasite was examined by transmission electron microscopy. Administration of (1) led to a considerable alteration of the laminated layer. Although a histoenzymatic study showed a decrease in alkaline phosphatase activity in the parasite, a precise mode of action for (1) cannot be proposed.
    International Journal of Pharmaceutics. 01/1993; 100:271-277.

Publication Stats

56 Citations
27.74 Total Impact Points

Institutions

  • 1995–2008
    • Claude Bernard University Lyon 1
      • Laboratoire de chimie
      Villeurbanne, Rhône-Alpes, France