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ABSTRACT: To investigate the effects of Liuweidihuang Decoction on the proliferation and melanin synthesis of cultured human melanocytes in vitro.
MTT assay was used to assess the proliferation of cultured human melanocytes after treatment with Liuweidihuang Decoction, and NaOH assay was employed to determine melanin synthesis.
Liuweidihuang Decoction at the concentration of 5%-15% did not affect the proliferation of the melanocytes (P>0.05), and the decoction at 20%-30% significantly inhibited the cell proliferation, especially at the optimal concentration of 30% (P<0.01). The decoction promoted melanin synthesis in the melanocytes at the concentration of 10%-15% with the optimal concentration of 15% (P<0.01) but failed to produce such an effect at 20%-30%.
Liuweidihuang Decoction exert a two-way regulation on the proliferation and melanin synthesis of cultured human melanocytes in vitro.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 04/2009; 29(4):701-3.
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ABSTRACT: To investigate the effects of Hominis placenta on melanocytes and tyrosinase activity in vitro.
MTT assay was used to assess the proliferation of melanocytes treated with Hominis placenta. Hunt method was employed to determine melanin synthesis and the oxidation rate of DL-dopa to evaluate the tyrosinase activity.
Hominis placenta promoted melanocyte proliferation (P<0.01) and significantly increased the cell number (P<0.01), melanin synthesis (P<0.01), with the optimal concentration of 0.1g/L (P<0.01), and also enhanced tyrosinase activity (P<0.01) at the optimal concentration of 1 g/L (P<0.01).
Hominis placenta promotes proliferation, melanin synthesis and tyrosinase activity of the melanocytes.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 10/2004; 24(9):1040-1, 1044.
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ABSTRACT: To find a relatively simple and safe method for quantitative assay of nuclear factor (NF)-kappa B activity.
Immunochemical staining of NF-kappa B/p65 subunit with its antibody was performed in human umbilical vein endothelial cells stimulated with advanced glycation end products-modified human serum albumin (AGE-HSA). The ratio of p65 subunit staining in the nuclei and cytoplasm was determined by means of imaging analysis, and the results were compared with those of electrophoretic mobility shift assay (EMSA).
NF-kappa B could be activated by AGE-HSA in a time- and dose-dependent manner, and the results obtained from immunochemical staining were consistent with those from EMSA.
p65 immunochemical staining and subsequent image analysis is feasible in the quantitative detection of NF-kappa B activation.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 06/2003; 23(5):498-500.
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ABSTRACT: To investigate the in vitro apoptosis-inducing effect of palmitic acid on pancreatic islet cells in primary culture, thereby to understand the role of palmitic acid in the pathogenesis of type 2 diabetes mellitus.
SD rat pancreatic islet cells were isolated and cultured in monolayer in vitro followed by incubation with stepwise diluted palmitic acid (0, 0.125, 0.25 mmol/L and 0.5 mmol/L respectively), and the insulin concentrations in the culture medium were determined by radio immunological methods. Morphological observation with a fluorescence microscope was conducted after double staining of the cells with PI/Hoechst 33342.
The glucose-stimulated insulin secretion was inhibited by palmitic acid at the concentration of 0.25 and 0.5 mmol/L, which also induced obvious cell apoptosis.
Palmitic acid is capable of inducing islet cell apoptosis in a dose-dependent manner.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 06/2003; 23(5):449-51.
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ABSTRACT: To evaluate the short-term effects of 3D conformal hypofractionated single high-dose radiotherapy for lung tumors.
Thirty-four patients with lung tumors that defied surgical removal were treated with 3D conformal hypofractionated single high-dose radiotherapy, and the short-term therapeutic effects and survival rates of the patients were assesses.
In 31 (91.2%) of the patients, the local tumor growth was successfully controlled, with the 1-, 2-, and 3- year survival rates of 88.2% (30/34), 35.3% (12/34), and 26.5% (9/34), respectively.
3D conformal hypofractionated single high-dose radiotherapy is effective and feasible approach for unresectable lung tumors judging from the short-term effects, but its long-term efficacy awaits further study.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 04/2003; 23(3):269-70.
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ABSTRACT: A genetic algorithm is proposed to detect target geometric figures in an given image. Float-point encoding was adopted to process the parameters of a geometric figures to be detected. On the basis of classical Hough transform, a fitness function was obtained for each individual task, and the individuals with the highest fitness function were identified and copied into the cohort of the next generation. For the rest of the individuals, operation with multi-point crossover or uniform mutation was performed to form new individuals in the next generation. When the termination conditions for this genetic algorithm were met, the best-fitted individual was decoded and output as the parameters of the detected geometric figures. This algorithm can eliminate noise interference with good convergence and accurate results, and may save time and storage space during relatively easily programmed computation in comparison with classical Hough transform.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 04/2003; 23(3):248-50.
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ABSTRACT: To investigate in vivo the enhancement by mifepristone (RU486) of the antitumor effect of cisplatin (DDP) on human ovarian carcinoma.
mouse models bearing xenografted cisplatin-resistant ovarian carcinoma were established, in which the changes in tumor volume and the morphology of the implanted tumors were compared before and after treatment with RU486 and DDP. The expression of glucosylceramide synthase (GcS) mRNA was detected by reverse transcriptase-PCR.
The morphology of the tumors in RU486 plus DDP group was significantly different as compared with that of the control group. In comparison with exclusive DDP treatment, combined treatment with RU486 and DDP resulted in significantly greater inhibition rates of the tumors (70.48% vs 21.55%, P < 0.01). GcS mRNA expression was lowered in both RU486 plus DDP and RU486 groups, especially in the former group, as compared with that in control group.
At a non-toxic dose, RU486 may enhance the sensitivity of tumor cells to cisplatin in vivo, possibly through the mechanism of inhibiting GcS expression at the mRNA level.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 03/2003; 23(3):242-4.
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ABSTRACT: To investigate the effects of Angelica sinensis (Oliv.) on melanocytes and tyrosinase activity.
MTT method and cleavage by NaOH were employed to measure the proliferation and melanin synthesis of melanocytes, respectively. Tyrasinase activity assessment was performed by measuring the rate of oxidation of DL-dopa.
Angelica sinensis (Oliv.) was found to promote melanocytic proliferation (P < 0.05), resulting in a statistically significant increase in the cell counts (P < 0.05). It also enhanced melanin synthesis (P < 0.05) and tryosinase activity (P < 0.05) of the melanocytes, and the most potent effects were achieved at the concentration of 1 g/L (P < 0.05).
Angelica sinensis (Oliv.) promotes melanocytic proliferation, melanin synthesis and tyrosinase activity, which may be the mechanism for validating its clinical use in the treatment of skin pigmentation.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 03/2003; 23(3):239-41.
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ABSTRACT: To study the effects of the decoction of Tribulus terrestris L on tyrosinase activity and melanogenesis.
MTT was employed to study the proliferation of human melanocytes cultured in vitro, and tyrosinase activity was estimated by measuring the rate of oxidation of DL-dopa, after the cells were treated with different concentrations of decoction of Tribulus terrestris L.
The treatment with the decoction increased the amount of melanin at higher concentrations but act to the reverse effect at lower concentrations, with the best concentrations for promoting and inhibiting the cell growth being 1.5 mg/ml (P<0.05) and 0.5 mg/ml (P<0.05) respectively. At high concentrations the decoction enhanced the tyrosinase activity that was inhibited at low concentrations. The best concentrations for enhancing and inhibiting tyrosinase activity were 100 mg/ml (P<0.05) and 10 mg/ml (P<0.05) respectively.
Tribulus terrestris L decoction exercises a two-way regulation on the activity of tyrosinase and the proliferation of melanocytes.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 11/2002; 22(11):1017-9.
