[Show abstract][Hide abstract] ABSTRACT: Amaranth, quínoa and chía are naturally gluten-free products that may be used in a celiac diet. An ELISA, using R-Biopharm RIDASCREEN gliadin, was used to determine a possible cross contamination with gliadins. Thirty-seven samples of foods with these ingredients were analyzed. Nine samples had levels higher than 20 mglKg, the maximum gluten level established by Codex Alimentarius: three of them were cereal bars with the inscription is in TACCî and/or ìwithout gluteni, two were cereal bars without inscriptions about gluten content, one was a mixture of ground seeds, others were pop amaranth and quínoa crops (sold at retail) and the last was an amaranth flour which was labeled ifor celiac patients. Twenty-eight remaining samples had gluten content below 20 mglKg. Foods elaborated with amaranth, quínoa and/or chía are suitable for celiac patients. However, the manufacturers must apply good manufacturing practices in all the different steps in gluten-free foodstuff production and celiac patients should not buy these products when they are sold at retail, because of possible cross contamination that can occur at the stores.
[Show abstract][Hide abstract] ABSTRACT: 19th International Congress of Nutrition: Abstracts: October 4-9, 2009, Bangkok, Thailand: Poster Presentations Part II: P98: Nutrition Monitoring & Evaluation III.
Annals of Nutrition and Metabolism 01/2009; 55(Supp 1):479-480. · 1.66 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of the present work was to study how a diet in which cereals were the only protein source would affect B and T lymphocytes and a cell population positive for thymus-expressed chemokine (TECK) in the intestinal villi of growing rats.
Wistar rats were fed a 6.5% precooked maize protein diet for 18-20 d (M group). An age-matched control group received stock diet (C group). Body weight (grams) was determined, ponderal growth rate (grams per day per 100 g) was calculated, and intestines were removed and processed by Saint-Marie's technique. Tissue sections were studied by indirect immunofluorescence. CD5(+) T cells and the T-cell subsets TCRalphabeta(+), TCRgammadelta(+), CD4(+), CD8alpha(+), and CD8beta(+) in the lamina propria (LP) and intraepithelium, in addition to immunoglobulin A-positive B cells in the LP were determined (n cells/30 fields were read). In addition, the presence of the TECK(+) cell population was qualitatively assessed.
The M versus C group showed statistically significant differences in body weight and ponderal growth rate. The number of immunoglobulin A-positive B cells in the LP and the CD5(+) T cells and CD4(+), CD8alpha(+), CD8beta(+), TCRalphabeta(+), and TCRgammadelta(+) T-cell subpopulations of the M group in the LP and intraepithelium of the gut villi were significantly decreased compared with the C group (P < 0.001). The M group also showed differences in the size and cellularity of the gut villi and in the distribution of TECK.
The results show that intake of a low concentration of a low-quality dietary protein as the only source of protein produces an important disorder in the mucosal immunity of experimental rats.
[Show abstract][Hide abstract] ABSTRACT: hypercholesterolemia, hypercortisolemia and low levels of essential fatty acids, oestrogens and antioxidant vitamins are more prevalent in patients with anorexia nervosa (AN) than in the general population.
This study aims (1) to compare cardiovascular risk parameters in-patients with AN and controls, and (2) to compare the parameters in-patients on admission and at four month follow up.
Blood samples and anthropometry were taken from patients with AN on admission (N=30) and matched controls (N=30). Twenty one patients were re-tested after four months of treatment.
Total cholesterol, LDL, Apo B and fibrinogen concentrations were elevated in patients on admission compared with controls, while retinol and tocopherol were decreased. Low levels of T3, T4 and estradiol were correlated with increased cholesterol values. After treatment there was a tendency for most of the abnormal markers to normalise. However, HDL levels decreased leaving patients with an undesirable lipid profile.
Cardiovascular disease is not commonly a problem in these patients, however, with age, and without treatment, the cardiovascular risk may increase.
The international journal of psychiatric nursing research 10/2007; 13(1):1531-45.
[Show abstract][Hide abstract] ABSTRACT: In the present paper we analyzed the effect caused by different recovery diets enriched with n-3 polyunsaturated fatty acids (PUFA n-3) on thymus and serum lipid pattern. Severe depleted weanling Wistar rats (D) were divided in three groups that received during 10 days a 20% casein diet supplemented with EPA+DHA (group Cas), a 20% protein milk diet prepared using a commercial reduced-fat product enriched with linolenic and linoleic acids (group L) and a 20% casein diet as control group C. Cas and L gave each other 24 mg/day of PUFA n-3 being the ratio n-6/n-3 8.1/1 and 7.6/1, respectively. Thymus was removed and weighted and cell number were determined; blood was recollected and Total cholesterol, triacylglycerol, HDL and LDL-cholesterol fractions and myristic, palmitic, stearic, oleic, linoleic, linolenic, araquidonic, EPA and DHA fatty acid concentrations were measured in serum. Statistical analysis was performed using Anova test. Cell number were higher (p<0.01) in Cas (44.48+/-8.20) and in L (56.45+/-14.72) when compared to group D (1.80+/-0.70) and group C (23.70+/-4.04). L presented lower values of cholesterol, HDL and LDL-cholesterol (p<0.01) and higher values of triacylglycerol (p<0.05) when compared to Cas, being EPA (p<0.05) and DHA (p<0.01) higher in Cas. Being PUFA n-3 contribution the same in Cas and L, both diets were able to reverse the thymic athropy presenting a different hipolipemic behavior due to the different sources of PUFA n-3 used in the diets.
Archivos latinoamericanos de nutrición 06/2007; 57(2):146-54. · 0.24 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Comparative study between two different sources of n-3 poliunsaturated fatty acids and it effect on thymus and lipid profile in rats. In the present paper we analyzed the effect caused by different recovery diets enriched with n-3 polyunsaturated fatty acids (PUFA n-3) on thymus and serum lipid pattern. Severe depleted weanling Wistar rats (D) were divided in three groups that received during 10 days a 20% casein diet supplemented with EPA+DHA (group Cas), a 20% protein milk diet prepared using a commercial reduced-fat product enriched with linolenic and linoleic acids (group L) and a 20% casein diet as control group C. Cas and L gave each other 24 mg/day of PUFA n-3 being the ratio n-6/n-3 8.1/ 1 and 7.6/1, respectively. Thymus was removed and weighted and cell number were determined; blood was recollected and Total cholesterol, triacylglycerol, HDL and LDL-cholesterol fractions and myristic, palmitic, stearic, oleic, linoleic, linolenic, araquidonic, EPA and DHA fatty acid concentrations were measured in serum. Statistical analysis was performed using Anova test. Cell number were higher (p
Archivos latinoamericanos de nutrición 06/2007; 57(2). · 0.24 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Studies of the immunologic function in adult obese humans and experimental models indicate that excess adiposity is associated with impairments in host defense mechanisms. The aim of this work was to analyze the secretory and humoral immune system in obese children (n = 105, 55 boys, 50 girls ), between 6 and 13 years of age. Samples of non-stimulated saliva and whole blood were collected from fasting patients. Total salivary IgA (IgAsal), serum C3 complement (C3c) and Immunoglobulin A (IgA) were determined by quantitative radial immunodifussion on agar gel layers ( Diffu-plate, Biocienti;fica SA ). Results, expressed as mg/dl, were compared to laboratory reference values from healthy children of either sex in the same range of age that belong to the same socioeconomic class (n = 60). Data (Mean +/- 1 SD) of the whole population were: IgAsal: 11.4 +/- 4.8 vs 14.8 +/- 6.9; C3c: 190.7 +/- 53.1 vs 126.3 +/- 45.5; IgA: 194.5 +/- 101.5 vs 157.2 +/- 19.9. Data distribution showed higher frecuencies near the zone of the highest reference values for serum C3c; when results of IgA and IgAsal were expressed as percentage of the mean reference value, 51% and 48.6% of the whole studied population presented data lower than 100% and 75% respectively. These results show a compromised secretory immune system without incidence of clinical symptoms and infections, whereas humoral immunity might not be profoundly affected.
The Journal of nutritional biochemistry 10/2002; 13(9):539. · 4.29 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We studied the effect of a low-quality dietary protein on cellular proliferation and maturation in the thymus of growing rats over time.
After weaning Wistar rats were fed a diet containing 6.5 g/100 g of corn flour for 6, 10, 18, and 45 d (M groups). For comparison, other rats were fed a diet containing 6.5 g/100 g of casein (Cas groups), and well-nourished age-matched control rats were fed a commercial laboratory diet (C groups). Food intake, body weight, thymus weight, total number of thymocytes, and the percentages of CD43(+) and Thy1(+) thymocyte phenotypic antigen determinants were measured.
M versus Cas and C groups showed significant differences (P < 0.01) in body and thymus weights after 6 d of feeding, and the total number of thymocytes and the percentages of CD43(+) and Thy1(+) were significantly lower after 10 d of feeding. The results indicated that consuming a cereal diet for short or long periods causes thymus atrophy in growing rats, with significant reductions in the total number of T-cells concomitant with increases in the number of immature thymocytes.
The data showed that, in addition to low-protein concentration, low-quality dietary protein is a limiting factor in certain steps of cellular intrathymic pathways, probably related to the requirement of specific amino acids for optimal immune response.
[Show abstract][Hide abstract] ABSTRACT: OBJECTIVES: Nutrition disorders caused by a 6.5% maize protein diet (M), unbalanced in its indispensable amino acid pattern, provokes an arrest on cellular proliferation and maturation in the thymus of growing rats. We investigated the effect of diet supplementation with different amounts of ω3 polyunsaturated fatty acid (M = 0 mg/d, M1 = 12 mg/d, M2 = 24 mg/d, respectively) on thymus, plasma lipid concentrations, and hepatic tissue.METHODS: A well-nourished age-matched control group received stock diet from weaning. The animals (8 to 10 per group) received the diets for 9 d. At the end of the experimental period, they were killed, thymuses were removed, and cell number, absolute number of T cells labeled by the monoclonal antibody W3/13, plasma lipid profile (total cholesterol, triacylglycerols, high- and low-density lipoprotein cholesterols), and oxidative stress in hepatic tissue were determined.RESULTS: Only M2 reached the values of the control group when cell number and absolute number of T cells were compared. No statistical differences were observed among the M, M1, M2, and control group when high- and low-density lipoprotein cholesterols and hepatic lipid peroxidation were considered.CONCLUSIONS: The supplementation of a 6.5% maize protein diet with 24 mg/d of ω3 polyunsaturated fatty acid can recover the proliferation and absolute number of T cells labeled with W3/13 without affecting lipid profile and hepatic lipid peroxidation.
[Show abstract][Hide abstract] ABSTRACT: Severe protein deprivation at weaning, may cause a stop in cellular proliferation and absolute number of T cells W3/13+ in the thymus of growing rats. The administration of a 20 g/100 casein recovery diet supplemented with 24 mg of n-3 PUFA during 9 days, counteracted this effect suggesting for these essential nutrients a dose-dependent response. No changes were observed in hemostastic factors such as protrombine time (QUICK) and partial active tromboplastine time (KPTT). Even though total plasma lipids showed no changes, the LDL-cholesterol fraction presented a low increment in agreement with international data. Daily administration of this supplemented diet did not show an increase in hepatic lipid peroxidation in the experimental group, suggesting that natural antioxidants and vitamin E provided by the diet might be playing a protective role.
Archivos latinoamericanos de nutrición 04/1999; 49(1):26-30. · 0.24 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Previous studies from our laboratory have shown that the intake of a low quality dietary protein during either a short or a long period, causes thymus atrophy of growing rats with a significant reduction in the number of cells and the mature T cell population. Moreover, thymus atrophy was associated with Zinc deficiency. The aim of this study was to determine if the intake of this diet induced a concomitant Zinc deficiency. Rats of the Wistar strain were fed from weaning and during 10 days a 6.5% maize protein diet (M10) or a 6.5% casein diet (C10). An age-matched control group was run simultaneously (C). The only dietary variable was the protein quality, being the Zinc content the same. Food intake (g/day) was similar: (M10: 6.5±1.4, C10: 6.8±1.1). Zn levels were determined by atomic absorption spectrophotometry in plasma (P), whole blood (WB), liver (L) and thymus (T) and expressed as μg/ml and μg/g wet tissue, respectively. No differences were found in WB (M10: 3.85±0.24, C10: 3.88±1.00, C: 3.67±0.34) nor L (M10: 42.50±11.30, C10: 36.30±6.70, C: 39.10±8.60). However, P levels decreased in M10 and C10 when compared to C (M10: 1.84±0.63, C10: 1.79±0.41, C: 2.43±0.37, P<0.04) while a significant increase in thymus Zn was only found in M10 compared to C10 and C (M10: 40.00±13.20, C10: 26.50±2.70, C: 27.10±3.60, p<0.01). These results show that the intake of a low quality dietary protein might alter the distribution of plasma Zinc with a concomitant uptake of Zinc by the thymus.
[Show abstract][Hide abstract] ABSTRACT: Severe protein deprivation at weaning provokes an arrest of cellular proliferation and maturation, and an increase of ADA and PNP activities in the thymus of growing rats. A 20% casein diet fed during 9 days was enough to reverse the effect on ADA and PNP. The supplementation with 24 mg/d of n-3 PUFA, was able to recover thymus' cellular proliferation and maturation.