Applied microbiology 10/1964; 12:412-7.
Source: PubMed

ABSTRACT Over 300 bacterial strains were isolated from seven samples of activated sludge by plating on sewage agar. Gram-negative bacteria of the genera Zoogloea and Comamonas predominated. Many isolates (51%) showed sudanophilic inclusions of poly-beta-hydroxybutyric acid, whereas 34% accumulated iodophilic material on media containing starch. A large number required either vitamins or amino acids, or both, for growth. None of the isolates tested for their ability to bring about changes in autoclaved sewage produced an effluent comparable in quality to the activated sludge control, although the Zoogloea did produce activated sludgelike flocs. A study of 150 bacterial strains isolated from raw sewage revealed that they differed from the sludge isolates in several respects. Coliforms, which constitute nearly a quarter of the sewage isolates, were rarely encountered in sludge.

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    ABSTRACT: Summary In this study the gas chromatographic method of Poly-β-hydroxy butyric acid determination of Braunegg et al. (1978), was optimised for activated sludge samples. The poly-β-hydroxy butyric acid was extracted and quantified gravimetrically to confirm the accuracy of the gas chromatographic method. The authenticity of the extracted material was confirmed by several methods. It was also confirmed that the mixed liquor of an activated sludge process did not interfere with the esterification. The sample size required was 25 ml of mix liquor, or 50 mg of freeze-dried sludge.
    European Journal of Applied Microbiology and Biotechnology 09/1981; 13(1):62-63.
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    ABSTRACT: Many studies have described alternatives to the BOD5 standard method, with substantial decreases in incubation time observed. However, most of these have not maintained the features that make the BOD5 assay so relevant - a high level of substrate bio-oxidation and use of wastewater treatment plant (WWTP) sludge as the biocatalyst. Two recently described ferricyanide-mediated (FM)-BOD assays, one for trade wastes and one for WWTP influents and treated effluents, satisfy these criteria and were investigated further here for their suitability for use with diverse biocatalysts. Both FM-BOD assays responded proportionately to increasing substrate concentration with sludges from 11 different WWTPs and temporally (months to years) using sludges from a single WWTP, confirming the broad applicability of both assays. Sludges from four WWTPs were selected as biocatalysts for each FM-BOD assay to compare FM-BOD equivalent values with BOD5 (three different sludge seeds) measurements for 12 real wastewater samples (six per assay). Strong and significant relationships were established for both FM-BOD assays. This study has demonstrated that sludge sourced from many WWTPs may be used as the biocatalyst in either FM-BOD assay, as it is in the BOD5 assay. The industry potential of these findings is substantial given the widespread use of the BOD5 assay, the dramatically decreased incubation period (3-6h) and the superior analytical range of both assays compared to the standard BOD5 assay.
    Talanta 07/2014; 125:293-300. · 3.50 Impact Factor
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    Journal of Environmental Engineering 09/2002; 128(9). · 1.22 Impact Factor


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