High rate of Simkania negevensis among Canadian inuit infants hospitalized with lower respiratory tract infections.

Pediatric Infectious Disease Unit, Soroka University Medical Center, Ben-Gurion University, Beer-Sheva, Israel.
Scandinavian Journal of Infectious Diseases (Impact Factor: 1.71). 02/2003; 35(8):506-8. DOI: 10.1080/00365540310014648
Source: PubMed

ABSTRACT To determine the prevalence of Simkania negevensis in causing pulmonary infections in children, nasopharyngeal washes were obtained from 22 infants hospitalized with acute bronchiolitis in the Baffin Island, Canada. 14 (63.6%) were positive for S. negevensis. Mixed infections with other respiratory viruses were common. All patients recovered without specific antibiotic treatment. Even though a high prevalence of S. negevensis was found, this organism may potentially well be an opportunistic agent rather than a true pathogen.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Community-acquired pneumonia (CAP) occurs more often in early childhood than at almost any other age. Many microorganisms are associated with pneumonia, but individual pathogens are difficult to identify, which poses problems in antibiotic management. This article reviews the common as well as new, emerging pathogens, as well as the guidelines for management of pediatric CAP. Current guidelines for pediatric CAP continue to recommend the use of high-dose amoxicillin for bacterial CAP and azithromycin for suspected atypical CAP (usually caused by Mycoplasma pneumoniae) in children.
    Pediatric Clinics of North America 04/2013; 60(2):437-453. · 1.78 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Deuterium trapping in graphite under high flux plasma exposure at high temperatures was studied. A target of MPG-8 fine grain graphite was exposed to plasma beam in LENTA device at zero potential. The average ion flux was of about 1021D/m2s with a maximum in the center. The temperature was 1473K in the center and 873K outside the beam spot. After irradiation, the target was sectioned along and across the surface and studied by SEM, NRA, and TDS. Deuterium profiles measured by NRA showed a peak close to the surface and long tails in the bulk. TDS of samples taken for the front and back sides of the target were very similar. It is suggested, that D retention at the plasma irradiation is mainly controlled by trapping from the gas phase, locally created in the graphite pores by plasma ions injection and subsequent flow of gas to the back side through internal porosity.
    Journal of Nuclear Materials 01/2011; 417(1):616-619. · 2.02 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Free-living amoebae are distributed worldwide and are frequently in contact with humans and animals. As cysts, they can survive in very harsh conditions and resist biocides and most disinfection procedures. Several microorganisms, called amoeba-resisting microorganisms (ARMs), have evolved to survive and multiply within these protozoa. Among them are many important pathogens, such as Legionella and Mycobacteria, and also several newly discovered Chlamydia-related bacteria, such as Parachlamydia acanthamoebae, Estrella lausannensis, Simkania negevensis or Waddlia chondrophila whose pathogenic role towards human or animal is strongly suspected. Amoebae represent an evolutionary crib for their resistant microorganisms since they can exchange genetic material with other ARMs and develop virulence traits that will be further used to infect other professional phagocytes. Moreover, amoebae constitute an ideal tool to isolate strict intracellular microorganisms from complex microbiota, since they will feed on other fast-growing bacteria, such as coliforms potentially present in the investigated samples. The paradigm that ARMs are likely resistant to macrophages, another phagocytic cell, and that they are likely virulent towards humans and animals is only partially true. Indeed, we provide examples of the Chlamydiales order that challenge this assumption and suggest that the ability to multiply in protozoa does not strictly correlate with pathogenicity and that we should rather use the ability to replicate in multiple and diverse eukaryotic cells as an indirect marker of virulence towards mammals. Thus, cell-culture-based microbial culturomics should be used in the future to try to discover new pathogenic bacterial species.
    Environmental Microbiology Reports 08/2014; 6(4):309-324. · 3.26 Impact Factor