The Effects of Serotonin on the Differentiation of Neurons Producing Vasoactive Intestinal Polypeptide in the Suprachiasmatic Nucleus of the Rat

P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, 8 Baltiiskaya Street, 125315 Moscow, Russia.
Neuroscience and Behavioral Physiology 10/2003; 33(7):729-33. DOI: 10.1023/A:1024477227552
Source: PubMed


The morphogenetic influences of serotonin on the differentiation of neurons synthesizing vasoactive intestinal polypeptide (VIP) in the suprachiasmatic nucleus were studied in rats. This was addressed by comparative morphofunctional analysis of VIP neurons in adult rats whose brains developed prenatally in conditions of normal and deficient serotonin metabolism. Serotonin deficiency was created in fetuses by treatment of their mothers with p-chlorophenylalanine (PCPA). Pregnant females in controls were treated with 0.9% NaCl. VIP neurons in experimental and control animals were found to show no differences in VIP mRNA concentrations and, probably, in the level of VIP synthesis. However, inhibition of serotonin synthesis led to an increase in the number of VIP-immunoreactive neurons and an increase in the VIP concentration within these cells. This was not associated with any change in neuron size, which was an indicator of the absence of functional hypertrophy accompanying activation of specific synthesis. Comparison of the data obtained here showed that during prenatal ontogenesis, serotonin has an imprinting influence on the differentiation of VIP neurons and is probably involved in the formation of the mechanism of VIP secretion.

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    • "ained as collateral symptomatology to possible VN autoimmune dysfunction . Blockade of 5 - HT could elicit symptomatology consistent with a VN autoimmune disorders . This effect may be explained by the role of serotonin in controlling VIP release mechanisms . Altered VIP expression may occur prenatally through serotonin imprinting in ontogenesis ( Mirochnik et al . 2003 ) suggesting implications for monitoring the use of selective serotonin uptake inhibitor ( SSRI ) and tri - cyclic anti - depressants in pregnancy . However , decreased 5 - HT1A receptor numbers and affinity are noted in CFS particularly in the hippocampus ( Cleare et al . 2005 ) possibly indicating heterogenous modulating relationships"
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