Article

Immunohistochemistry and c-kit gene analysis in determining malignancy in gastrointestinal stromal tumors.

Department of Surgery, Showa University Fujigaoka Hospital, Yokohama, Japan.
Hepato-gastroenterology (impact factor: 0.66). 50(53):1431-5. pp.1431-5
Source: PubMed

ABSTRACT Usefulness of immunohistochemistry and c-kit proto-oncogene mutation was examined for determining malignancy in 24 cases of gastrointestinal stromal tumors.
Cases were histologically diagnosed and subjected to immunohistochemical staining and c-kit gene analysis. All parameters were compared to prognosis.
There were significant differences in tumor size, central necrosis, mitotic activity and histological diagnosis between recurrent and non-recurrent cases. Positivity to KIT staining was 100% in recurrent and 87.5% in non-recurrent cases. Positivity to Ki-67 and p53 staining were significantly higher in recurrent cases than in non-recurrent cases. Mutations in exon 11 of the c-kit gene were significantly more frequent in recurrent cases than in non-recurrent cases.
Histological diagnosis, tumor size, central necrosis, and mitotic activity were reconfirmed to be indicators for recurrence of gastrointestinal stromal tumors. Furthermore, it is suggested that positivity of Ki-67 and p53 immunostaining and c-kit gene mutation also need to be done for prediction of recurrence.

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Keywords

c-kit gene
 
c-kit gene analysis
 
c-kit gene mutation
 
c-kit proto-oncogene mutation
 
Cases
 
frequent
 
gastrointestinal stromal tumors
 
histological diagnosis
 
immunohistochemical staining
 
immunohistochemistry
 
indicators
 
KIT staining
 
mitotic activity
 
Mutations
 
non-recurrent cases
 
p53 immunostaining
 
p53 staining
 
positivity
 
recurrent cases
 
tumor size